Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan

GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in...

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Autores principales: Khan, Anwar Sheed, Ali, Sajid, Khan, Muhammad Tahir, Ahmed, Sajjad, Khattak, Yasir, Abduljabbar, Irfan, Muhammad, Sajjad, Wasim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175715/
https://www.ncbi.nlm.nih.gov/pubmed/29752010
http://dx.doi.org/10.1016/j.bjm.2018.02.011
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author Khan, Anwar Sheed
Ali, Sajid
Khan, Muhammad Tahir
Ahmed, Sajjad
Khattak, Yasir
Abduljabbar
Irfan, Muhammad
Sajjad, Wasim
author_facet Khan, Anwar Sheed
Ali, Sajid
Khan, Muhammad Tahir
Ahmed, Sajjad
Khattak, Yasir
Abduljabbar
Irfan, Muhammad
Sajjad, Wasim
author_sort Khan, Anwar Sheed
collection PubMed
description GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.
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spelling pubmed-61757152018-10-09 Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan Khan, Anwar Sheed Ali, Sajid Khan, Muhammad Tahir Ahmed, Sajjad Khattak, Yasir Abduljabbar Irfan, Muhammad Sajjad, Wasim Braz J Microbiol Research Paper GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy. Elsevier 2018-04-27 /pmc/articles/PMC6175715/ /pubmed/29752010 http://dx.doi.org/10.1016/j.bjm.2018.02.011 Text en © 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Khan, Anwar Sheed
Ali, Sajid
Khan, Muhammad Tahir
Ahmed, Sajjad
Khattak, Yasir
Abduljabbar
Irfan, Muhammad
Sajjad, Wasim
Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
title Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
title_full Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
title_fullStr Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
title_full_unstemmed Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
title_short Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
title_sort comparison of genexpert mtb/rif assay and led-fm microscopy for the diagnosis of extra pulmonary tuberculosis in khyber pakhtunkhwa, pakistan
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175715/
https://www.ncbi.nlm.nih.gov/pubmed/29752010
http://dx.doi.org/10.1016/j.bjm.2018.02.011
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