Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175715/ https://www.ncbi.nlm.nih.gov/pubmed/29752010 http://dx.doi.org/10.1016/j.bjm.2018.02.011 |
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author | Khan, Anwar Sheed Ali, Sajid Khan, Muhammad Tahir Ahmed, Sajjad Khattak, Yasir Abduljabbar Irfan, Muhammad Sajjad, Wasim |
author_facet | Khan, Anwar Sheed Ali, Sajid Khan, Muhammad Tahir Ahmed, Sajjad Khattak, Yasir Abduljabbar Irfan, Muhammad Sajjad, Wasim |
author_sort | Khan, Anwar Sheed |
collection | PubMed |
description | GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy. |
format | Online Article Text |
id | pubmed-6175715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61757152018-10-09 Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan Khan, Anwar Sheed Ali, Sajid Khan, Muhammad Tahir Ahmed, Sajjad Khattak, Yasir Abduljabbar Irfan, Muhammad Sajjad, Wasim Braz J Microbiol Research Paper GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy. Elsevier 2018-04-27 /pmc/articles/PMC6175715/ /pubmed/29752010 http://dx.doi.org/10.1016/j.bjm.2018.02.011 Text en © 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper Khan, Anwar Sheed Ali, Sajid Khan, Muhammad Tahir Ahmed, Sajjad Khattak, Yasir Abduljabbar Irfan, Muhammad Sajjad, Wasim Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan |
title | Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan |
title_full | Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan |
title_fullStr | Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan |
title_full_unstemmed | Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan |
title_short | Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan |
title_sort | comparison of genexpert mtb/rif assay and led-fm microscopy for the diagnosis of extra pulmonary tuberculosis in khyber pakhtunkhwa, pakistan |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175715/ https://www.ncbi.nlm.nih.gov/pubmed/29752010 http://dx.doi.org/10.1016/j.bjm.2018.02.011 |
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