In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster

The deleterious alteration of protein structure and function due to the oxidation of methionine residues has been studied extensively in age-associated neurodegenerative disorders such as Alzheimer’s and Parkinson’s Disease. Methionine sulfoxide reductases (MSR) have three well-characterized biologi...

Descripción completa

Detalles Bibliográficos
Autores principales: Bruce, Lindsay, Singkornrat, Diana, Wilson, Kelsey, Hausman, William, Robbins, Kelli, Huang, Lingxi, Foss, Katie, Binninger, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262642/
https://www.ncbi.nlm.nih.gov/pubmed/30388828
http://dx.doi.org/10.3390/antiox7110155
_version_ 1783375151353561088
author Bruce, Lindsay
Singkornrat, Diana
Wilson, Kelsey
Hausman, William
Robbins, Kelli
Huang, Lingxi
Foss, Katie
Binninger, David
author_facet Bruce, Lindsay
Singkornrat, Diana
Wilson, Kelsey
Hausman, William
Robbins, Kelli
Huang, Lingxi
Foss, Katie
Binninger, David
author_sort Bruce, Lindsay
collection PubMed
description The deleterious alteration of protein structure and function due to the oxidation of methionine residues has been studied extensively in age-associated neurodegenerative disorders such as Alzheimer’s and Parkinson’s Disease. Methionine sulfoxide reductases (MSR) have three well-characterized biological functions. The most commonly studied function is the reduction of oxidized methionine residues back into functional methionine thus, often restoring biological function to proteins. Previous studies have successfully overexpressed and silenced MSR activity in numerous model organisms correlating its activity to longevity and oxidative stress. In the present study, we have characterized in vivo effects of MSR deficiency in Drosophila. Interestingly, we found no significant phenotype in animals lacking either methionine sulfoxide reductase A (MSRA) or methionine sulfoxide reductase B (MSRB). However, Drosophila lacking any known MSR activity exhibited a prolonged larval third instar development and a shortened lifespan. These data suggest an essential role of MSR in key biological processes.
format Online
Article
Text
id pubmed-6262642
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62626422018-11-29 In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster Bruce, Lindsay Singkornrat, Diana Wilson, Kelsey Hausman, William Robbins, Kelli Huang, Lingxi Foss, Katie Binninger, David Antioxidants (Basel) Article The deleterious alteration of protein structure and function due to the oxidation of methionine residues has been studied extensively in age-associated neurodegenerative disorders such as Alzheimer’s and Parkinson’s Disease. Methionine sulfoxide reductases (MSR) have three well-characterized biological functions. The most commonly studied function is the reduction of oxidized methionine residues back into functional methionine thus, often restoring biological function to proteins. Previous studies have successfully overexpressed and silenced MSR activity in numerous model organisms correlating its activity to longevity and oxidative stress. In the present study, we have characterized in vivo effects of MSR deficiency in Drosophila. Interestingly, we found no significant phenotype in animals lacking either methionine sulfoxide reductase A (MSRA) or methionine sulfoxide reductase B (MSRB). However, Drosophila lacking any known MSR activity exhibited a prolonged larval third instar development and a shortened lifespan. These data suggest an essential role of MSR in key biological processes. MDPI 2018-11-01 /pmc/articles/PMC6262642/ /pubmed/30388828 http://dx.doi.org/10.3390/antiox7110155 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bruce, Lindsay
Singkornrat, Diana
Wilson, Kelsey
Hausman, William
Robbins, Kelli
Huang, Lingxi
Foss, Katie
Binninger, David
In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster
title In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster
title_full In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster
title_fullStr In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster
title_full_unstemmed In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster
title_short In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster
title_sort in vivo effects of methionine sulfoxide reductase deficiency in drosophila melanogaster
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262642/
https://www.ncbi.nlm.nih.gov/pubmed/30388828
http://dx.doi.org/10.3390/antiox7110155
work_keys_str_mv AT brucelindsay invivoeffectsofmethioninesulfoxidereductasedeficiencyindrosophilamelanogaster
AT singkornratdiana invivoeffectsofmethioninesulfoxidereductasedeficiencyindrosophilamelanogaster
AT wilsonkelsey invivoeffectsofmethioninesulfoxidereductasedeficiencyindrosophilamelanogaster
AT hausmanwilliam invivoeffectsofmethioninesulfoxidereductasedeficiencyindrosophilamelanogaster
AT robbinskelli invivoeffectsofmethioninesulfoxidereductasedeficiencyindrosophilamelanogaster
AT huanglingxi invivoeffectsofmethioninesulfoxidereductasedeficiencyindrosophilamelanogaster
AT fosskatie invivoeffectsofmethioninesulfoxidereductasedeficiencyindrosophilamelanogaster
AT binningerdavid invivoeffectsofmethioninesulfoxidereductasedeficiencyindrosophilamelanogaster