In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster
The deleterious alteration of protein structure and function due to the oxidation of methionine residues has been studied extensively in age-associated neurodegenerative disorders such as Alzheimer’s and Parkinson’s Disease. Methionine sulfoxide reductases (MSR) have three well-characterized biologi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262642/ https://www.ncbi.nlm.nih.gov/pubmed/30388828 http://dx.doi.org/10.3390/antiox7110155 |
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author | Bruce, Lindsay Singkornrat, Diana Wilson, Kelsey Hausman, William Robbins, Kelli Huang, Lingxi Foss, Katie Binninger, David |
author_facet | Bruce, Lindsay Singkornrat, Diana Wilson, Kelsey Hausman, William Robbins, Kelli Huang, Lingxi Foss, Katie Binninger, David |
author_sort | Bruce, Lindsay |
collection | PubMed |
description | The deleterious alteration of protein structure and function due to the oxidation of methionine residues has been studied extensively in age-associated neurodegenerative disorders such as Alzheimer’s and Parkinson’s Disease. Methionine sulfoxide reductases (MSR) have three well-characterized biological functions. The most commonly studied function is the reduction of oxidized methionine residues back into functional methionine thus, often restoring biological function to proteins. Previous studies have successfully overexpressed and silenced MSR activity in numerous model organisms correlating its activity to longevity and oxidative stress. In the present study, we have characterized in vivo effects of MSR deficiency in Drosophila. Interestingly, we found no significant phenotype in animals lacking either methionine sulfoxide reductase A (MSRA) or methionine sulfoxide reductase B (MSRB). However, Drosophila lacking any known MSR activity exhibited a prolonged larval third instar development and a shortened lifespan. These data suggest an essential role of MSR in key biological processes. |
format | Online Article Text |
id | pubmed-6262642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62626422018-11-29 In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster Bruce, Lindsay Singkornrat, Diana Wilson, Kelsey Hausman, William Robbins, Kelli Huang, Lingxi Foss, Katie Binninger, David Antioxidants (Basel) Article The deleterious alteration of protein structure and function due to the oxidation of methionine residues has been studied extensively in age-associated neurodegenerative disorders such as Alzheimer’s and Parkinson’s Disease. Methionine sulfoxide reductases (MSR) have three well-characterized biological functions. The most commonly studied function is the reduction of oxidized methionine residues back into functional methionine thus, often restoring biological function to proteins. Previous studies have successfully overexpressed and silenced MSR activity in numerous model organisms correlating its activity to longevity and oxidative stress. In the present study, we have characterized in vivo effects of MSR deficiency in Drosophila. Interestingly, we found no significant phenotype in animals lacking either methionine sulfoxide reductase A (MSRA) or methionine sulfoxide reductase B (MSRB). However, Drosophila lacking any known MSR activity exhibited a prolonged larval third instar development and a shortened lifespan. These data suggest an essential role of MSR in key biological processes. MDPI 2018-11-01 /pmc/articles/PMC6262642/ /pubmed/30388828 http://dx.doi.org/10.3390/antiox7110155 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bruce, Lindsay Singkornrat, Diana Wilson, Kelsey Hausman, William Robbins, Kelli Huang, Lingxi Foss, Katie Binninger, David In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster |
title | In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster |
title_full | In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster |
title_fullStr | In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster |
title_full_unstemmed | In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster |
title_short | In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster |
title_sort | in vivo effects of methionine sulfoxide reductase deficiency in drosophila melanogaster |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262642/ https://www.ncbi.nlm.nih.gov/pubmed/30388828 http://dx.doi.org/10.3390/antiox7110155 |
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