Graphene-Induced Osteogenic Differentiation Is Mediated by the Integrin/FAK Axis
Graphene is capable of promoting osteogenesis without chemical induction. Nevertheless, the underlying mechanism(s) remain largely unknown. The objectives here were: (i) to assess whether graphene scaffolds are capable of supporting osteogenesis in vivo and; (ii) to ascertain the participation of th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387117/ https://www.ncbi.nlm.nih.gov/pubmed/30699966 http://dx.doi.org/10.3390/ijms20030574 |
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author | Xie, Han Cao, Tong Franco-Obregón, Alfredo Rosa, Vinicius |
author_facet | Xie, Han Cao, Tong Franco-Obregón, Alfredo Rosa, Vinicius |
author_sort | Xie, Han |
collection | PubMed |
description | Graphene is capable of promoting osteogenesis without chemical induction. Nevertheless, the underlying mechanism(s) remain largely unknown. The objectives here were: (i) to assess whether graphene scaffolds are capable of supporting osteogenesis in vivo and; (ii) to ascertain the participation of the integrin/FAK mechanotransduction axis during the osteogenic differentiation induced by graphene. MSC-impregnated graphene scaffolds (n = 6) were implanted into immunocompromised mice (28 days). Alternatively, MSCs were seeded onto PDMS substrates (modulus of elasticity = 130, 830 and 1300 kPa) coated with a single monomolecular layer of graphene and cultured in basal medium (10 days). The ensuing expressions of FAK-p397, integrin, ROCK1, F-actin, Smad p1/5, RUNX2, OCN and OPN were evaluated by Western blot (n = 3). As controls, MSCs were plated onto uncoated PDMS in the presence of mechanotransduction inhibitors (echistatin, Y27632 and DMH1). MSC-impregnated graphene scaffolds exhibited positive immunoexpression of bone-related markers (RUNX2 and OPN) without the assistance of osteogenic inducers. In vitro, regardless of the stiffness of the underlying PDMS substrate, MSCs seeded onto graphene-coated PDMS substrates demonstrated higher expressions of all tested osteogenic and integrin/FAK proteins tested compared to MSCs seeded onto PDMS alone. Hence, graphene promotes osteogenesis via the activation of the mechanosensitive integrin/FAK axis. |
format | Online Article Text |
id | pubmed-6387117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63871172019-02-27 Graphene-Induced Osteogenic Differentiation Is Mediated by the Integrin/FAK Axis Xie, Han Cao, Tong Franco-Obregón, Alfredo Rosa, Vinicius Int J Mol Sci Article Graphene is capable of promoting osteogenesis without chemical induction. Nevertheless, the underlying mechanism(s) remain largely unknown. The objectives here were: (i) to assess whether graphene scaffolds are capable of supporting osteogenesis in vivo and; (ii) to ascertain the participation of the integrin/FAK mechanotransduction axis during the osteogenic differentiation induced by graphene. MSC-impregnated graphene scaffolds (n = 6) were implanted into immunocompromised mice (28 days). Alternatively, MSCs were seeded onto PDMS substrates (modulus of elasticity = 130, 830 and 1300 kPa) coated with a single monomolecular layer of graphene and cultured in basal medium (10 days). The ensuing expressions of FAK-p397, integrin, ROCK1, F-actin, Smad p1/5, RUNX2, OCN and OPN were evaluated by Western blot (n = 3). As controls, MSCs were plated onto uncoated PDMS in the presence of mechanotransduction inhibitors (echistatin, Y27632 and DMH1). MSC-impregnated graphene scaffolds exhibited positive immunoexpression of bone-related markers (RUNX2 and OPN) without the assistance of osteogenic inducers. In vitro, regardless of the stiffness of the underlying PDMS substrate, MSCs seeded onto graphene-coated PDMS substrates demonstrated higher expressions of all tested osteogenic and integrin/FAK proteins tested compared to MSCs seeded onto PDMS alone. Hence, graphene promotes osteogenesis via the activation of the mechanosensitive integrin/FAK axis. MDPI 2019-01-29 /pmc/articles/PMC6387117/ /pubmed/30699966 http://dx.doi.org/10.3390/ijms20030574 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xie, Han Cao, Tong Franco-Obregón, Alfredo Rosa, Vinicius Graphene-Induced Osteogenic Differentiation Is Mediated by the Integrin/FAK Axis |
title | Graphene-Induced Osteogenic Differentiation Is Mediated by the Integrin/FAK Axis |
title_full | Graphene-Induced Osteogenic Differentiation Is Mediated by the Integrin/FAK Axis |
title_fullStr | Graphene-Induced Osteogenic Differentiation Is Mediated by the Integrin/FAK Axis |
title_full_unstemmed | Graphene-Induced Osteogenic Differentiation Is Mediated by the Integrin/FAK Axis |
title_short | Graphene-Induced Osteogenic Differentiation Is Mediated by the Integrin/FAK Axis |
title_sort | graphene-induced osteogenic differentiation is mediated by the integrin/fak axis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387117/ https://www.ncbi.nlm.nih.gov/pubmed/30699966 http://dx.doi.org/10.3390/ijms20030574 |
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