Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability

The Nitrogen Use Efficiency (NUE) of grain cereals depends on nitrate (NO(3)(−)) uptake from the soil, translocation to the aerial parts, nitrogen (N) assimilation and remobilization to the grains. Brachypodium distachyon has been proposed as a model species to identify the molecular players and mec...

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Autores principales: David, L. C., Girin, T., Fleurisson, E., Phommabouth, E., Mahfoudhi, A., Citerne, S., Berquin, P., Daniel-Vedele, F., Krapp, A., Ferrario-Méry, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405861/
https://www.ncbi.nlm.nih.gov/pubmed/30846873
http://dx.doi.org/10.1038/s41598-019-40569-8
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author David, L. C.
Girin, T.
Fleurisson, E.
Phommabouth, E.
Mahfoudhi, A.
Citerne, S.
Berquin, P.
Daniel-Vedele, F.
Krapp, A.
Ferrario-Méry, S.
author_facet David, L. C.
Girin, T.
Fleurisson, E.
Phommabouth, E.
Mahfoudhi, A.
Citerne, S.
Berquin, P.
Daniel-Vedele, F.
Krapp, A.
Ferrario-Méry, S.
author_sort David, L. C.
collection PubMed
description The Nitrogen Use Efficiency (NUE) of grain cereals depends on nitrate (NO(3)(−)) uptake from the soil, translocation to the aerial parts, nitrogen (N) assimilation and remobilization to the grains. Brachypodium distachyon has been proposed as a model species to identify the molecular players and mechanisms that affects these processes, for the improvement of temperate C3 cereals. We report on the developmental, physiological and grain-characteristic responses of the Bd21-3 accession of Brachypodium to variations in NO(3)(−) availability. As previously described in wheat and barley, we show that vegetative growth, shoot/root ratio, tiller formation, spike development, tissue NO(3)(−) and N contents, grain number per plant, grain yield and grain N content are sensitive to pre- and/or post-anthesis NO(3)(−) supply. We subsequently described constitutive and NO(3)(−)-inducible components of both High and Low Affinity Transport Systems (HATS and LATS) for root NO(3)(−) uptake, and BdNRT2/3 candidate genes potentially involved in the HATS. Taken together, our data validate Brachypodium Bd21-3 as a model to decipher cereal N nutrition. Apparent specificities such as high grain N content, strong post-anthesis NO(3)(−) uptake and efficient constitutive HATS, further identify Brachypodium as a direct source of knowledge for crop improvement.
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spelling pubmed-64058612019-03-11 Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability David, L. C. Girin, T. Fleurisson, E. Phommabouth, E. Mahfoudhi, A. Citerne, S. Berquin, P. Daniel-Vedele, F. Krapp, A. Ferrario-Méry, S. Sci Rep Article The Nitrogen Use Efficiency (NUE) of grain cereals depends on nitrate (NO(3)(−)) uptake from the soil, translocation to the aerial parts, nitrogen (N) assimilation and remobilization to the grains. Brachypodium distachyon has been proposed as a model species to identify the molecular players and mechanisms that affects these processes, for the improvement of temperate C3 cereals. We report on the developmental, physiological and grain-characteristic responses of the Bd21-3 accession of Brachypodium to variations in NO(3)(−) availability. As previously described in wheat and barley, we show that vegetative growth, shoot/root ratio, tiller formation, spike development, tissue NO(3)(−) and N contents, grain number per plant, grain yield and grain N content are sensitive to pre- and/or post-anthesis NO(3)(−) supply. We subsequently described constitutive and NO(3)(−)-inducible components of both High and Low Affinity Transport Systems (HATS and LATS) for root NO(3)(−) uptake, and BdNRT2/3 candidate genes potentially involved in the HATS. Taken together, our data validate Brachypodium Bd21-3 as a model to decipher cereal N nutrition. Apparent specificities such as high grain N content, strong post-anthesis NO(3)(−) uptake and efficient constitutive HATS, further identify Brachypodium as a direct source of knowledge for crop improvement. Nature Publishing Group UK 2019-03-07 /pmc/articles/PMC6405861/ /pubmed/30846873 http://dx.doi.org/10.1038/s41598-019-40569-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
David, L. C.
Girin, T.
Fleurisson, E.
Phommabouth, E.
Mahfoudhi, A.
Citerne, S.
Berquin, P.
Daniel-Vedele, F.
Krapp, A.
Ferrario-Méry, S.
Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability
title Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability
title_full Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability
title_fullStr Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability
title_full_unstemmed Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability
title_short Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability
title_sort developmental and physiological responses of brachypodium distachyon to fluctuating nitrogen availability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405861/
https://www.ncbi.nlm.nih.gov/pubmed/30846873
http://dx.doi.org/10.1038/s41598-019-40569-8
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