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The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer

Preclinical trials of medullary thyroid cancer (MTC) therapeutics require both in vitro and in vivo analyses. Human tumour xenografted rodent models, which are considered the ‘gold standard’ to study and validate the efficacy and toxicity of lead compounds before translation to clinical trials, are...

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Autores principales: Ghaffari-Tabrizi-Wizsy, Nassim, Passegger, Christina Angelika, Nebel, Laura, Krismer, Fabian, Herzer-Schneidhofer, Gudrun, Schwach, Gert, Pfragner, Roswitha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479196/
https://www.ncbi.nlm.nih.gov/pubmed/30913539
http://dx.doi.org/10.1530/EC-18-0431
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author Ghaffari-Tabrizi-Wizsy, Nassim
Passegger, Christina Angelika
Nebel, Laura
Krismer, Fabian
Herzer-Schneidhofer, Gudrun
Schwach, Gert
Pfragner, Roswitha
author_facet Ghaffari-Tabrizi-Wizsy, Nassim
Passegger, Christina Angelika
Nebel, Laura
Krismer, Fabian
Herzer-Schneidhofer, Gudrun
Schwach, Gert
Pfragner, Roswitha
author_sort Ghaffari-Tabrizi-Wizsy, Nassim
collection PubMed
description Preclinical trials of medullary thyroid cancer (MTC) therapeutics require both in vitro and in vivo analyses. Human tumour xenografted rodent models, which are considered the ‘gold standard’ to study and validate the efficacy and toxicity of lead compounds before translation to clinical trials, are very expensive, subject to organismal variability and ethical controversies. The avian chorioallantoic membrane (CAM) assay provides an alternative versatile, cost-effective and ethically less objectionable short-term, in vivo model for reliable screening of drugs. In this work, we grafted two MTC cell lines and patient-derived MTC tumour samples onto the avian CAM and characterised the resulted tumours histologically and immunohistochemically. Our findings provide the evidence that the CAM assay is a suitable model for studying the pathophysiology of MTC and can even be used as in vivo system for drug testing.
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spelling pubmed-64791962019-04-26 The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer Ghaffari-Tabrizi-Wizsy, Nassim Passegger, Christina Angelika Nebel, Laura Krismer, Fabian Herzer-Schneidhofer, Gudrun Schwach, Gert Pfragner, Roswitha Endocr Connect Research Preclinical trials of medullary thyroid cancer (MTC) therapeutics require both in vitro and in vivo analyses. Human tumour xenografted rodent models, which are considered the ‘gold standard’ to study and validate the efficacy and toxicity of lead compounds before translation to clinical trials, are very expensive, subject to organismal variability and ethical controversies. The avian chorioallantoic membrane (CAM) assay provides an alternative versatile, cost-effective and ethically less objectionable short-term, in vivo model for reliable screening of drugs. In this work, we grafted two MTC cell lines and patient-derived MTC tumour samples onto the avian CAM and characterised the resulted tumours histologically and immunohistochemically. Our findings provide the evidence that the CAM assay is a suitable model for studying the pathophysiology of MTC and can even be used as in vivo system for drug testing. Bioscientifica Ltd 2019-03-26 /pmc/articles/PMC6479196/ /pubmed/30913539 http://dx.doi.org/10.1530/EC-18-0431 Text en © 2019 The authors http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. (http://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Research
Ghaffari-Tabrizi-Wizsy, Nassim
Passegger, Christina Angelika
Nebel, Laura
Krismer, Fabian
Herzer-Schneidhofer, Gudrun
Schwach, Gert
Pfragner, Roswitha
The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer
title The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer
title_full The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer
title_fullStr The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer
title_full_unstemmed The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer
title_short The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer
title_sort avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479196/
https://www.ncbi.nlm.nih.gov/pubmed/30913539
http://dx.doi.org/10.1530/EC-18-0431
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