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The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer
Preclinical trials of medullary thyroid cancer (MTC) therapeutics require both in vitro and in vivo analyses. Human tumour xenografted rodent models, which are considered the ‘gold standard’ to study and validate the efficacy and toxicity of lead compounds before translation to clinical trials, are...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bioscientifica Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479196/ https://www.ncbi.nlm.nih.gov/pubmed/30913539 http://dx.doi.org/10.1530/EC-18-0431 |
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author | Ghaffari-Tabrizi-Wizsy, Nassim Passegger, Christina Angelika Nebel, Laura Krismer, Fabian Herzer-Schneidhofer, Gudrun Schwach, Gert Pfragner, Roswitha |
author_facet | Ghaffari-Tabrizi-Wizsy, Nassim Passegger, Christina Angelika Nebel, Laura Krismer, Fabian Herzer-Schneidhofer, Gudrun Schwach, Gert Pfragner, Roswitha |
author_sort | Ghaffari-Tabrizi-Wizsy, Nassim |
collection | PubMed |
description | Preclinical trials of medullary thyroid cancer (MTC) therapeutics require both in vitro and in vivo analyses. Human tumour xenografted rodent models, which are considered the ‘gold standard’ to study and validate the efficacy and toxicity of lead compounds before translation to clinical trials, are very expensive, subject to organismal variability and ethical controversies. The avian chorioallantoic membrane (CAM) assay provides an alternative versatile, cost-effective and ethically less objectionable short-term, in vivo model for reliable screening of drugs. In this work, we grafted two MTC cell lines and patient-derived MTC tumour samples onto the avian CAM and characterised the resulted tumours histologically and immunohistochemically. Our findings provide the evidence that the CAM assay is a suitable model for studying the pathophysiology of MTC and can even be used as in vivo system for drug testing. |
format | Online Article Text |
id | pubmed-6479196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Bioscientifica Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-64791962019-04-26 The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer Ghaffari-Tabrizi-Wizsy, Nassim Passegger, Christina Angelika Nebel, Laura Krismer, Fabian Herzer-Schneidhofer, Gudrun Schwach, Gert Pfragner, Roswitha Endocr Connect Research Preclinical trials of medullary thyroid cancer (MTC) therapeutics require both in vitro and in vivo analyses. Human tumour xenografted rodent models, which are considered the ‘gold standard’ to study and validate the efficacy and toxicity of lead compounds before translation to clinical trials, are very expensive, subject to organismal variability and ethical controversies. The avian chorioallantoic membrane (CAM) assay provides an alternative versatile, cost-effective and ethically less objectionable short-term, in vivo model for reliable screening of drugs. In this work, we grafted two MTC cell lines and patient-derived MTC tumour samples onto the avian CAM and characterised the resulted tumours histologically and immunohistochemically. Our findings provide the evidence that the CAM assay is a suitable model for studying the pathophysiology of MTC and can even be used as in vivo system for drug testing. Bioscientifica Ltd 2019-03-26 /pmc/articles/PMC6479196/ /pubmed/30913539 http://dx.doi.org/10.1530/EC-18-0431 Text en © 2019 The authors http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. (http://creativecommons.org/licenses/by-nc/4.0/) |
spellingShingle | Research Ghaffari-Tabrizi-Wizsy, Nassim Passegger, Christina Angelika Nebel, Laura Krismer, Fabian Herzer-Schneidhofer, Gudrun Schwach, Gert Pfragner, Roswitha The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer |
title | The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer |
title_full | The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer |
title_fullStr | The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer |
title_full_unstemmed | The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer |
title_short | The avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer |
title_sort | avian chorioallantoic membrane as an alternative tool to study medullary thyroid cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479196/ https://www.ncbi.nlm.nih.gov/pubmed/30913539 http://dx.doi.org/10.1530/EC-18-0431 |
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