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Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells

Research on statins highlights their potent cytotoxicity against cancer cells and their potential for cancer prevention. The aim of the current study was to examine whether loading lovastatin (LVS) in zein (ZN) nanoparticles (NPs) would potentiate the anti-proliferative effects of LVS and enhance it...

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Autores principales: Alhakamy, Nabil A., Ahmed, Osama A.A., Aldawsari, Hibah M., Alfaifi, Mohammad Y., Eid, Basma G., Abdel-Naim, Ashraf B., Fahmy, Usama A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888474/
https://www.ncbi.nlm.nih.gov/pubmed/31752085
http://dx.doi.org/10.3390/ijms20225788
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author Alhakamy, Nabil A.
Ahmed, Osama A.A.
Aldawsari, Hibah M.
Alfaifi, Mohammad Y.
Eid, Basma G.
Abdel-Naim, Ashraf B.
Fahmy, Usama A.
author_facet Alhakamy, Nabil A.
Ahmed, Osama A.A.
Aldawsari, Hibah M.
Alfaifi, Mohammad Y.
Eid, Basma G.
Abdel-Naim, Ashraf B.
Fahmy, Usama A.
author_sort Alhakamy, Nabil A.
collection PubMed
description Research on statins highlights their potent cytotoxicity against cancer cells and their potential for cancer prevention. The aim of the current study was to examine whether loading lovastatin (LVS) in zein (ZN) nanoparticles (NPs) would potentiate the anti-proliferative effects of LVS and enhance its proliferation-inhibiting activity in HepG2 cells. LVS-ZN NPs were prepared and showed excellent characteristics, with respect to their particle size, zeta potential, diffusion, and entrapment efficiency. In addition, they showed the most potent anti-proliferative activity against HepG2 cells. ZN alone showed an observable anti-proliferative that was significantly higher than that of raw LVS. Furthermore, LVS uptake by HepG2 cells was greatly enhanced by the formulation in ZN. A cell cycle analysis indicated that LVS induced a significant cell accumulation in the G2/M and pre-G phases. In this regard, the LVS–ZN NPs exhibited the highest potency. The accumulation in the pre-G phase indicated an enhanced pro-apoptotic activity of the prepared formula. The cells incubated with the LVS-ZN NPs showed the highest percentage of cells with annexin-V positive staining. In addition, the same incubations showed the highest content of caspase-3 enzyme in comparison to raw LVS or ZN. Thus, the loading of LVS in ZN nanoparticles enhances its anti-proliferative activity against HepG2 cells, which is attributed, at least partly, to the enhanced cellular uptake and the induction of apoptosis.
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spelling pubmed-68884742019-12-09 Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells Alhakamy, Nabil A. Ahmed, Osama A.A. Aldawsari, Hibah M. Alfaifi, Mohammad Y. Eid, Basma G. Abdel-Naim, Ashraf B. Fahmy, Usama A. Int J Mol Sci Article Research on statins highlights their potent cytotoxicity against cancer cells and their potential for cancer prevention. The aim of the current study was to examine whether loading lovastatin (LVS) in zein (ZN) nanoparticles (NPs) would potentiate the anti-proliferative effects of LVS and enhance its proliferation-inhibiting activity in HepG2 cells. LVS-ZN NPs were prepared and showed excellent characteristics, with respect to their particle size, zeta potential, diffusion, and entrapment efficiency. In addition, they showed the most potent anti-proliferative activity against HepG2 cells. ZN alone showed an observable anti-proliferative that was significantly higher than that of raw LVS. Furthermore, LVS uptake by HepG2 cells was greatly enhanced by the formulation in ZN. A cell cycle analysis indicated that LVS induced a significant cell accumulation in the G2/M and pre-G phases. In this regard, the LVS–ZN NPs exhibited the highest potency. The accumulation in the pre-G phase indicated an enhanced pro-apoptotic activity of the prepared formula. The cells incubated with the LVS-ZN NPs showed the highest percentage of cells with annexin-V positive staining. In addition, the same incubations showed the highest content of caspase-3 enzyme in comparison to raw LVS or ZN. Thus, the loading of LVS in ZN nanoparticles enhances its anti-proliferative activity against HepG2 cells, which is attributed, at least partly, to the enhanced cellular uptake and the induction of apoptosis. MDPI 2019-11-18 /pmc/articles/PMC6888474/ /pubmed/31752085 http://dx.doi.org/10.3390/ijms20225788 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alhakamy, Nabil A.
Ahmed, Osama A.A.
Aldawsari, Hibah M.
Alfaifi, Mohammad Y.
Eid, Basma G.
Abdel-Naim, Ashraf B.
Fahmy, Usama A.
Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells
title Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells
title_full Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells
title_fullStr Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells
title_full_unstemmed Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells
title_short Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells
title_sort encapsulation of lovastatin in zein nanoparticles exhibits enhanced apoptotic activity in hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888474/
https://www.ncbi.nlm.nih.gov/pubmed/31752085
http://dx.doi.org/10.3390/ijms20225788
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