Cargando…
Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits
Wnt signaling plays an important role in the growth and development of hair follicles (HFs). Among the signaling molecules, Wnt10b was shown to promote the differentiation of primary skin epithelial cells toward the hair shaft and inner root sheath of the HF cells in mice in vitro. Whisker HFs were...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000364/ https://www.ncbi.nlm.nih.gov/pubmed/31961392 http://dx.doi.org/10.1042/BSR20191248 |
_version_ | 1783494026183311360 |
---|---|
author | Wu, Zhenyu Zhu, Yanli Liu, Hongli Liu, Gongyan Li, Fuchang |
author_facet | Wu, Zhenyu Zhu, Yanli Liu, Hongli Liu, Gongyan Li, Fuchang |
author_sort | Wu, Zhenyu |
collection | PubMed |
description | Wnt signaling plays an important role in the growth and development of hair follicles (HFs). Among the signaling molecules, Wnt10b was shown to promote the differentiation of primary skin epithelial cells toward the hair shaft and inner root sheath of the HF cells in mice in vitro. Whisker HFs were isolated from Rex rabbits and cultured in vitro to measure hair shaft growth. Meanwhile, dermal papilla cells (DPCs) were isolated and cultured in vitro. Treatment with AdWnt10b or the Wnt/β-Catenin Pathway inhibitor, XAV939, assessed the DPCs proliferation by CCK-8 assay. And the cell cycle was also analyzed by flow cytometry. We found that Wnt10b could promote elongation of the hair shaft, whereas XAV-939 treatment could eliminated this phenomenon. AdWnt10b treatment promoted the proliferation and induced G1/S transition of DPCs. AdWnt10b stimulation up-regulated β-Catenin protein in DPCs. Inhibition of Wnt/β-Catenin signaling by XAV-939 could decreased the basal and Wnt10b-enhanced proliferation of DPCs. And could also suppress the cell cycle progression in DPCs. In summary, our study demonstrates that Wnt10b could promote HFs growth and proliferation of DPCs via the Wnt/β-Catenin signaling pathway in Rex rabbits. |
format | Online Article Text |
id | pubmed-7000364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70003642020-02-12 Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits Wu, Zhenyu Zhu, Yanli Liu, Hongli Liu, Gongyan Li, Fuchang Biosci Rep Cell Cycle, Growth & Proliferation Wnt signaling plays an important role in the growth and development of hair follicles (HFs). Among the signaling molecules, Wnt10b was shown to promote the differentiation of primary skin epithelial cells toward the hair shaft and inner root sheath of the HF cells in mice in vitro. Whisker HFs were isolated from Rex rabbits and cultured in vitro to measure hair shaft growth. Meanwhile, dermal papilla cells (DPCs) were isolated and cultured in vitro. Treatment with AdWnt10b or the Wnt/β-Catenin Pathway inhibitor, XAV939, assessed the DPCs proliferation by CCK-8 assay. And the cell cycle was also analyzed by flow cytometry. We found that Wnt10b could promote elongation of the hair shaft, whereas XAV-939 treatment could eliminated this phenomenon. AdWnt10b treatment promoted the proliferation and induced G1/S transition of DPCs. AdWnt10b stimulation up-regulated β-Catenin protein in DPCs. Inhibition of Wnt/β-Catenin signaling by XAV-939 could decreased the basal and Wnt10b-enhanced proliferation of DPCs. And could also suppress the cell cycle progression in DPCs. In summary, our study demonstrates that Wnt10b could promote HFs growth and proliferation of DPCs via the Wnt/β-Catenin signaling pathway in Rex rabbits. Portland Press Ltd. 2020-02-04 /pmc/articles/PMC7000364/ /pubmed/31961392 http://dx.doi.org/10.1042/BSR20191248 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Cell Cycle, Growth & Proliferation Wu, Zhenyu Zhu, Yanli Liu, Hongli Liu, Gongyan Li, Fuchang Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits |
title | Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits |
title_full | Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits |
title_fullStr | Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits |
title_full_unstemmed | Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits |
title_short | Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits |
title_sort | wnt10b promotes hair follicles growth and dermal papilla cells proliferation via wnt/β-catenin signaling pathway in rex rabbits |
topic | Cell Cycle, Growth & Proliferation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000364/ https://www.ncbi.nlm.nih.gov/pubmed/31961392 http://dx.doi.org/10.1042/BSR20191248 |
work_keys_str_mv | AT wuzhenyu wnt10bpromoteshairfolliclesgrowthanddermalpapillacellsproliferationviawntbcateninsignalingpathwayinrexrabbits AT zhuyanli wnt10bpromoteshairfolliclesgrowthanddermalpapillacellsproliferationviawntbcateninsignalingpathwayinrexrabbits AT liuhongli wnt10bpromoteshairfolliclesgrowthanddermalpapillacellsproliferationviawntbcateninsignalingpathwayinrexrabbits AT liugongyan wnt10bpromoteshairfolliclesgrowthanddermalpapillacellsproliferationviawntbcateninsignalingpathwayinrexrabbits AT lifuchang wnt10bpromoteshairfolliclesgrowthanddermalpapillacellsproliferationviawntbcateninsignalingpathwayinrexrabbits |