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Force-Dependent Binding Constants

[Image: see text] Life is an emergent property of transient interactions between biomolecules and other organic and inorganic molecules that somehow leads to harmony and order. Measurement and quantitation of these biological interactions are of value to scientists and are major goals of biochemistr...

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Detalles Bibliográficos
Autores principales: Wang, Yinan, Yan, Jie, Goult, Benjamin T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007008/
https://www.ncbi.nlm.nih.gov/pubmed/31315399
http://dx.doi.org/10.1021/acs.biochem.9b00453
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author Wang, Yinan
Yan, Jie
Goult, Benjamin T.
author_facet Wang, Yinan
Yan, Jie
Goult, Benjamin T.
author_sort Wang, Yinan
collection PubMed
description [Image: see text] Life is an emergent property of transient interactions between biomolecules and other organic and inorganic molecules that somehow leads to harmony and order. Measurement and quantitation of these biological interactions are of value to scientists and are major goals of biochemistry, as affinities provide insight into biological processes. In an organism, these interactions occur in the context of forces and the need for a consideration of binding affinities in the context of a changing mechanical landscape necessitates a new way to consider the biochemistry of protein–protein interactions. In the past few decades, the field of mechanobiology has exploded, as both the appreciation of, and the technical advances required to facilitate the study of, how forces impact biological processes have become evident. The aim of this review is to introduce the concept of force dependence of biomolecular interactions and the requirement to be able to measure force-dependent binding constants. The focus of this discussion will be on the mechanotransduction that occurs at the integrin-mediated adhesions with the extracellular matrix and the major mechanosensors talin and vinculin. However, the approaches that the cell uses to sense and respond to forces can be applied to other systems, and this therefore provides a general discussion of the force dependence of biomolecule interactions.
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spelling pubmed-70070082020-02-10 Force-Dependent Binding Constants Wang, Yinan Yan, Jie Goult, Benjamin T. Biochemistry [Image: see text] Life is an emergent property of transient interactions between biomolecules and other organic and inorganic molecules that somehow leads to harmony and order. Measurement and quantitation of these biological interactions are of value to scientists and are major goals of biochemistry, as affinities provide insight into biological processes. In an organism, these interactions occur in the context of forces and the need for a consideration of binding affinities in the context of a changing mechanical landscape necessitates a new way to consider the biochemistry of protein–protein interactions. In the past few decades, the field of mechanobiology has exploded, as both the appreciation of, and the technical advances required to facilitate the study of, how forces impact biological processes have become evident. The aim of this review is to introduce the concept of force dependence of biomolecular interactions and the requirement to be able to measure force-dependent binding constants. The focus of this discussion will be on the mechanotransduction that occurs at the integrin-mediated adhesions with the extracellular matrix and the major mechanosensors talin and vinculin. However, the approaches that the cell uses to sense and respond to forces can be applied to other systems, and this therefore provides a general discussion of the force dependence of biomolecule interactions. American Chemical Society 2019-07-18 2019-11-26 /pmc/articles/PMC7007008/ /pubmed/31315399 http://dx.doi.org/10.1021/acs.biochem.9b00453 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Wang, Yinan
Yan, Jie
Goult, Benjamin T.
Force-Dependent Binding Constants
title Force-Dependent Binding Constants
title_full Force-Dependent Binding Constants
title_fullStr Force-Dependent Binding Constants
title_full_unstemmed Force-Dependent Binding Constants
title_short Force-Dependent Binding Constants
title_sort force-dependent binding constants
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007008/
https://www.ncbi.nlm.nih.gov/pubmed/31315399
http://dx.doi.org/10.1021/acs.biochem.9b00453
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