In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles

Olive leaf extract is characterized by a high content of phenols and flavonoids (oleuropein, luteolin, and their derivatives). These compounds are defined as secondary metabolites and exert such as anti-inflammatory, antioxidant, and antimicrobial activities. We investigated the in vitro antifungal...

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Autores principales: Muzzalupo, Innocenzo, Badolati, Giuliana, Chiappetta, Adriana, Picci, Nevio, Muzzalupo, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062645/
https://www.ncbi.nlm.nih.gov/pubmed/32195234
http://dx.doi.org/10.3389/fbioe.2020.00151
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author Muzzalupo, Innocenzo
Badolati, Giuliana
Chiappetta, Adriana
Picci, Nevio
Muzzalupo, Rita
author_facet Muzzalupo, Innocenzo
Badolati, Giuliana
Chiappetta, Adriana
Picci, Nevio
Muzzalupo, Rita
author_sort Muzzalupo, Innocenzo
collection PubMed
description Olive leaf extract is characterized by a high content of phenols and flavonoids (oleuropein, luteolin, and their derivatives). These compounds are defined as secondary metabolites and exert such as anti-inflammatory, antioxidant, and antimicrobial activities. We investigated the in vitro antifungal activity of two olive leaf extracts (named EF1 and EF2) against a Fusarium proliferatum (AACC0215) strain that causes diseases to many economically important plants and synthesizing diverse mycotoxins. In this work, we aimed to identify the most appropriate concentration between the tested two olive leaf extracts to develop a safe, stable and efficient drug delivery system. Qualitative and quantitative analyses of the two olive leaf extracts by (HPLC) were performed. Furthermore, we also evaluated the antifungal effects of the two leaf extracts when encapsulated in chitosan-tripolyphosphate nanoparticles. The major compound in both EF1 and EF2 was oleuropein, with 336 and 603 mg/g, respectively, however, high concentrations of flavonoid were also present. EF1 and EF2 showed a concentration depended effect on F. proliferatum (AACC0215) viability. Our results showed a great efficacy of EF1/nanoparticles at the higher concentration tested (12X) against the target species. In this case, we observed an inhibition rate to both germination and growth of 87.96 and 58.13%, respectively. We suggest that EF1 olive leaf extracts, as free or encapsulated in chitosan-tripolyphosphate nanoparticles, could be used as fungicides to control plant diseases. Finally, future application of these findings may allow to reduce the dosage of fungicides potentially harmful to human health.
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spelling pubmed-70626452020-03-19 In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles Muzzalupo, Innocenzo Badolati, Giuliana Chiappetta, Adriana Picci, Nevio Muzzalupo, Rita Front Bioeng Biotechnol Bioengineering and Biotechnology Olive leaf extract is characterized by a high content of phenols and flavonoids (oleuropein, luteolin, and their derivatives). These compounds are defined as secondary metabolites and exert such as anti-inflammatory, antioxidant, and antimicrobial activities. We investigated the in vitro antifungal activity of two olive leaf extracts (named EF1 and EF2) against a Fusarium proliferatum (AACC0215) strain that causes diseases to many economically important plants and synthesizing diverse mycotoxins. In this work, we aimed to identify the most appropriate concentration between the tested two olive leaf extracts to develop a safe, stable and efficient drug delivery system. Qualitative and quantitative analyses of the two olive leaf extracts by (HPLC) were performed. Furthermore, we also evaluated the antifungal effects of the two leaf extracts when encapsulated in chitosan-tripolyphosphate nanoparticles. The major compound in both EF1 and EF2 was oleuropein, with 336 and 603 mg/g, respectively, however, high concentrations of flavonoid were also present. EF1 and EF2 showed a concentration depended effect on F. proliferatum (AACC0215) viability. Our results showed a great efficacy of EF1/nanoparticles at the higher concentration tested (12X) against the target species. In this case, we observed an inhibition rate to both germination and growth of 87.96 and 58.13%, respectively. We suggest that EF1 olive leaf extracts, as free or encapsulated in chitosan-tripolyphosphate nanoparticles, could be used as fungicides to control plant diseases. Finally, future application of these findings may allow to reduce the dosage of fungicides potentially harmful to human health. Frontiers Media S.A. 2020-03-03 /pmc/articles/PMC7062645/ /pubmed/32195234 http://dx.doi.org/10.3389/fbioe.2020.00151 Text en Copyright © 2020 Muzzalupo, Chiappetta, Badolati, Picci and Muzzalupo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Muzzalupo, Innocenzo
Badolati, Giuliana
Chiappetta, Adriana
Picci, Nevio
Muzzalupo, Rita
In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles
title In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles
title_full In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles
title_fullStr In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles
title_full_unstemmed In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles
title_short In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles
title_sort in vitro antifungal activity of olive (olea europaea) leaf extracts loaded in chitosan nanoparticles
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062645/
https://www.ncbi.nlm.nih.gov/pubmed/32195234
http://dx.doi.org/10.3389/fbioe.2020.00151
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