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Global mistranslation increases cell survival under stress in Escherichia coli

Mistranslation is typically deleterious for cells, although specific mistranslated proteins can confer a short-term benefit in a particular environment. However, given its large overall cost, the prevalence of high global mistranslation rates remains puzzling. Altering basal mistranslation levels of...

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Detalles Bibliográficos
Autores principales: Samhita, Laasya, Raval, Parth K., Agashe, Deepa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082066/
https://www.ncbi.nlm.nih.gov/pubmed/32150542
http://dx.doi.org/10.1371/journal.pgen.1008654
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author Samhita, Laasya
Raval, Parth K.
Agashe, Deepa
author_facet Samhita, Laasya
Raval, Parth K.
Agashe, Deepa
author_sort Samhita, Laasya
collection PubMed
description Mistranslation is typically deleterious for cells, although specific mistranslated proteins can confer a short-term benefit in a particular environment. However, given its large overall cost, the prevalence of high global mistranslation rates remains puzzling. Altering basal mistranslation levels of Escherichia coli in several ways, we show that generalized mistranslation enhances early survival under DNA damage, by rapidly activating the SOS response. Mistranslating cells maintain larger populations after exposure to DNA damage, and thus have a higher probability of sampling critical beneficial mutations. Both basal and artificially increased mistranslation increase the number of cells that are phenotypically tolerant and genetically resistant under DNA damage; they also enhance survival at high temperature. In contrast, decreasing the normal basal mistranslation rate reduces cell survival. This wide-ranging stress resistance relies on Lon protease, which is revealed as a key effector that induces the SOS response in addition to alleviating proteotoxic stress. The new links between error-prone protein synthesis, DNA damage, and generalised stress resistance indicate surprising coordination between intracellular stress responses and suggest a novel hypothesis to explain high global mistranslation rates.
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spelling pubmed-70820662020-03-24 Global mistranslation increases cell survival under stress in Escherichia coli Samhita, Laasya Raval, Parth K. Agashe, Deepa PLoS Genet Research Article Mistranslation is typically deleterious for cells, although specific mistranslated proteins can confer a short-term benefit in a particular environment. However, given its large overall cost, the prevalence of high global mistranslation rates remains puzzling. Altering basal mistranslation levels of Escherichia coli in several ways, we show that generalized mistranslation enhances early survival under DNA damage, by rapidly activating the SOS response. Mistranslating cells maintain larger populations after exposure to DNA damage, and thus have a higher probability of sampling critical beneficial mutations. Both basal and artificially increased mistranslation increase the number of cells that are phenotypically tolerant and genetically resistant under DNA damage; they also enhance survival at high temperature. In contrast, decreasing the normal basal mistranslation rate reduces cell survival. This wide-ranging stress resistance relies on Lon protease, which is revealed as a key effector that induces the SOS response in addition to alleviating proteotoxic stress. The new links between error-prone protein synthesis, DNA damage, and generalised stress resistance indicate surprising coordination between intracellular stress responses and suggest a novel hypothesis to explain high global mistranslation rates. Public Library of Science 2020-03-09 /pmc/articles/PMC7082066/ /pubmed/32150542 http://dx.doi.org/10.1371/journal.pgen.1008654 Text en © 2020 Samhita et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Samhita, Laasya
Raval, Parth K.
Agashe, Deepa
Global mistranslation increases cell survival under stress in Escherichia coli
title Global mistranslation increases cell survival under stress in Escherichia coli
title_full Global mistranslation increases cell survival under stress in Escherichia coli
title_fullStr Global mistranslation increases cell survival under stress in Escherichia coli
title_full_unstemmed Global mistranslation increases cell survival under stress in Escherichia coli
title_short Global mistranslation increases cell survival under stress in Escherichia coli
title_sort global mistranslation increases cell survival under stress in escherichia coli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082066/
https://www.ncbi.nlm.nih.gov/pubmed/32150542
http://dx.doi.org/10.1371/journal.pgen.1008654
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