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Global mistranslation increases cell survival under stress in Escherichia coli
Mistranslation is typically deleterious for cells, although specific mistranslated proteins can confer a short-term benefit in a particular environment. However, given its large overall cost, the prevalence of high global mistranslation rates remains puzzling. Altering basal mistranslation levels of...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082066/ https://www.ncbi.nlm.nih.gov/pubmed/32150542 http://dx.doi.org/10.1371/journal.pgen.1008654 |
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author | Samhita, Laasya Raval, Parth K. Agashe, Deepa |
author_facet | Samhita, Laasya Raval, Parth K. Agashe, Deepa |
author_sort | Samhita, Laasya |
collection | PubMed |
description | Mistranslation is typically deleterious for cells, although specific mistranslated proteins can confer a short-term benefit in a particular environment. However, given its large overall cost, the prevalence of high global mistranslation rates remains puzzling. Altering basal mistranslation levels of Escherichia coli in several ways, we show that generalized mistranslation enhances early survival under DNA damage, by rapidly activating the SOS response. Mistranslating cells maintain larger populations after exposure to DNA damage, and thus have a higher probability of sampling critical beneficial mutations. Both basal and artificially increased mistranslation increase the number of cells that are phenotypically tolerant and genetically resistant under DNA damage; they also enhance survival at high temperature. In contrast, decreasing the normal basal mistranslation rate reduces cell survival. This wide-ranging stress resistance relies on Lon protease, which is revealed as a key effector that induces the SOS response in addition to alleviating proteotoxic stress. The new links between error-prone protein synthesis, DNA damage, and generalised stress resistance indicate surprising coordination between intracellular stress responses and suggest a novel hypothesis to explain high global mistranslation rates. |
format | Online Article Text |
id | pubmed-7082066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-70820662020-03-24 Global mistranslation increases cell survival under stress in Escherichia coli Samhita, Laasya Raval, Parth K. Agashe, Deepa PLoS Genet Research Article Mistranslation is typically deleterious for cells, although specific mistranslated proteins can confer a short-term benefit in a particular environment. However, given its large overall cost, the prevalence of high global mistranslation rates remains puzzling. Altering basal mistranslation levels of Escherichia coli in several ways, we show that generalized mistranslation enhances early survival under DNA damage, by rapidly activating the SOS response. Mistranslating cells maintain larger populations after exposure to DNA damage, and thus have a higher probability of sampling critical beneficial mutations. Both basal and artificially increased mistranslation increase the number of cells that are phenotypically tolerant and genetically resistant under DNA damage; they also enhance survival at high temperature. In contrast, decreasing the normal basal mistranslation rate reduces cell survival. This wide-ranging stress resistance relies on Lon protease, which is revealed as a key effector that induces the SOS response in addition to alleviating proteotoxic stress. The new links between error-prone protein synthesis, DNA damage, and generalised stress resistance indicate surprising coordination between intracellular stress responses and suggest a novel hypothesis to explain high global mistranslation rates. Public Library of Science 2020-03-09 /pmc/articles/PMC7082066/ /pubmed/32150542 http://dx.doi.org/10.1371/journal.pgen.1008654 Text en © 2020 Samhita et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Samhita, Laasya Raval, Parth K. Agashe, Deepa Global mistranslation increases cell survival under stress in Escherichia coli |
title | Global mistranslation increases cell survival under stress in Escherichia coli |
title_full | Global mistranslation increases cell survival under stress in Escherichia coli |
title_fullStr | Global mistranslation increases cell survival under stress in Escherichia coli |
title_full_unstemmed | Global mistranslation increases cell survival under stress in Escherichia coli |
title_short | Global mistranslation increases cell survival under stress in Escherichia coli |
title_sort | global mistranslation increases cell survival under stress in escherichia coli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082066/ https://www.ncbi.nlm.nih.gov/pubmed/32150542 http://dx.doi.org/10.1371/journal.pgen.1008654 |
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