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Metabolic changes during respiratory syncytial virus infection of epithelial cells

Viral infections induce substantial metabolic changes in infected cells to optimize viral production while cells develop countermeasures to restrict that infection. Human respiratory syncytial virus (HRSV) is an infectious pathogen that causes severe lower respiratory tract infections (LRTI) in infa...

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Autores principales: Martín-Vicente, María, González-Riaño, Carolina, Barbas, Coral, Jiménez-Sousa, María Ángeles, Brochado-Kith, Oscar, Resino, Salvador, Martínez, Isidoro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098640/
https://www.ncbi.nlm.nih.gov/pubmed/32214395
http://dx.doi.org/10.1371/journal.pone.0230844
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author Martín-Vicente, María
González-Riaño, Carolina
Barbas, Coral
Jiménez-Sousa, María Ángeles
Brochado-Kith, Oscar
Resino, Salvador
Martínez, Isidoro
author_facet Martín-Vicente, María
González-Riaño, Carolina
Barbas, Coral
Jiménez-Sousa, María Ángeles
Brochado-Kith, Oscar
Resino, Salvador
Martínez, Isidoro
author_sort Martín-Vicente, María
collection PubMed
description Viral infections induce substantial metabolic changes in infected cells to optimize viral production while cells develop countermeasures to restrict that infection. Human respiratory syncytial virus (HRSV) is an infectious pathogen that causes severe lower respiratory tract infections (LRTI) in infants, the elderly, and immunocompromised adults for which no effective treatment or vaccine is currently available. In this study, variations in metabolite levels at different time points post-HRSV infection of epithelial cells were studied by untargeted metabolomics using liquid chromatography/mass spectrometry analysis of methanol cell extracts. Numerous metabolites were significantly upregulated after 18 hours post-infection, including nucleotides, amino acids, amino and nucleotide sugars, and metabolites of the central carbon pathway. In contrast, most lipid classes were downregulated. Additionally, increased levels of oxidized glutathione and polyamines were associated with oxidative stress in infected cells. These results show how HRSV infection influences cell metabolism to produce the energy and building blocks necessary for virus reproduction, suggesting potential therapeutic interventions against this virus.
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spelling pubmed-70986402020-04-03 Metabolic changes during respiratory syncytial virus infection of epithelial cells Martín-Vicente, María González-Riaño, Carolina Barbas, Coral Jiménez-Sousa, María Ángeles Brochado-Kith, Oscar Resino, Salvador Martínez, Isidoro PLoS One Research Article Viral infections induce substantial metabolic changes in infected cells to optimize viral production while cells develop countermeasures to restrict that infection. Human respiratory syncytial virus (HRSV) is an infectious pathogen that causes severe lower respiratory tract infections (LRTI) in infants, the elderly, and immunocompromised adults for which no effective treatment or vaccine is currently available. In this study, variations in metabolite levels at different time points post-HRSV infection of epithelial cells were studied by untargeted metabolomics using liquid chromatography/mass spectrometry analysis of methanol cell extracts. Numerous metabolites were significantly upregulated after 18 hours post-infection, including nucleotides, amino acids, amino and nucleotide sugars, and metabolites of the central carbon pathway. In contrast, most lipid classes were downregulated. Additionally, increased levels of oxidized glutathione and polyamines were associated with oxidative stress in infected cells. These results show how HRSV infection influences cell metabolism to produce the energy and building blocks necessary for virus reproduction, suggesting potential therapeutic interventions against this virus. Public Library of Science 2020-03-26 /pmc/articles/PMC7098640/ /pubmed/32214395 http://dx.doi.org/10.1371/journal.pone.0230844 Text en © 2020 Martín-Vicente et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Martín-Vicente, María
González-Riaño, Carolina
Barbas, Coral
Jiménez-Sousa, María Ángeles
Brochado-Kith, Oscar
Resino, Salvador
Martínez, Isidoro
Metabolic changes during respiratory syncytial virus infection of epithelial cells
title Metabolic changes during respiratory syncytial virus infection of epithelial cells
title_full Metabolic changes during respiratory syncytial virus infection of epithelial cells
title_fullStr Metabolic changes during respiratory syncytial virus infection of epithelial cells
title_full_unstemmed Metabolic changes during respiratory syncytial virus infection of epithelial cells
title_short Metabolic changes during respiratory syncytial virus infection of epithelial cells
title_sort metabolic changes during respiratory syncytial virus infection of epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098640/
https://www.ncbi.nlm.nih.gov/pubmed/32214395
http://dx.doi.org/10.1371/journal.pone.0230844
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