An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles

In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino...

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Detalles Bibliográficos
Autores principales: Ashtari, Parviz, He, Xiaoxiao, Wang, Kemin, Gong, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111873/
https://www.ncbi.nlm.nih.gov/pubmed/18970204
http://dx.doi.org/10.1016/j.talanta.2005.06.043
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author Ashtari, Parviz
He, Xiaoxiao
Wang, Kemin
Gong, Ping
author_facet Ashtari, Parviz
He, Xiaoxiao
Wang, Kemin
Gong, Ping
author_sort Ashtari, Parviz
collection PubMed
description In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5′-TMR-CGCATAGGGCCTCGTGATAC-3′) has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity.
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spelling pubmed-71118732020-04-02 An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles Ashtari, Parviz He, Xiaoxiao Wang, Kemin Gong, Ping Talanta Article In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5′-TMR-CGCATAGGGCCTCGTGATAC-3′) has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity. Elsevier B.V. 2005-09-15 2005-08-01 /pmc/articles/PMC7111873/ /pubmed/18970204 http://dx.doi.org/10.1016/j.talanta.2005.06.043 Text en Copyright © 2005 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ashtari, Parviz
He, Xiaoxiao
Wang, Kemin
Gong, Ping
An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles
title An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles
title_full An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles
title_fullStr An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles
title_full_unstemmed An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles
title_short An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles
title_sort efficient method for recovery of target ssdna based on amino-modified silica-coated magnetic nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111873/
https://www.ncbi.nlm.nih.gov/pubmed/18970204
http://dx.doi.org/10.1016/j.talanta.2005.06.043
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