Cargando…
An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles
In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2005
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111873/ https://www.ncbi.nlm.nih.gov/pubmed/18970204 http://dx.doi.org/10.1016/j.talanta.2005.06.043 |
_version_ | 1783513375867666432 |
---|---|
author | Ashtari, Parviz He, Xiaoxiao Wang, Kemin Gong, Ping |
author_facet | Ashtari, Parviz He, Xiaoxiao Wang, Kemin Gong, Ping |
author_sort | Ashtari, Parviz |
collection | PubMed |
description | In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5′-TMR-CGCATAGGGCCTCGTGATAC-3′) has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity. |
format | Online Article Text |
id | pubmed-7111873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71118732020-04-02 An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles Ashtari, Parviz He, Xiaoxiao Wang, Kemin Gong, Ping Talanta Article In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5′-TMR-CGCATAGGGCCTCGTGATAC-3′) has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity. Elsevier B.V. 2005-09-15 2005-08-01 /pmc/articles/PMC7111873/ /pubmed/18970204 http://dx.doi.org/10.1016/j.talanta.2005.06.043 Text en Copyright © 2005 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Ashtari, Parviz He, Xiaoxiao Wang, Kemin Gong, Ping An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles |
title | An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles |
title_full | An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles |
title_fullStr | An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles |
title_full_unstemmed | An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles |
title_short | An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles |
title_sort | efficient method for recovery of target ssdna based on amino-modified silica-coated magnetic nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111873/ https://www.ncbi.nlm.nih.gov/pubmed/18970204 http://dx.doi.org/10.1016/j.talanta.2005.06.043 |
work_keys_str_mv | AT ashtariparviz anefficientmethodforrecoveryoftargetssdnabasedonaminomodifiedsilicacoatedmagneticnanoparticles AT hexiaoxiao anefficientmethodforrecoveryoftargetssdnabasedonaminomodifiedsilicacoatedmagneticnanoparticles AT wangkemin anefficientmethodforrecoveryoftargetssdnabasedonaminomodifiedsilicacoatedmagneticnanoparticles AT gongping anefficientmethodforrecoveryoftargetssdnabasedonaminomodifiedsilicacoatedmagneticnanoparticles AT ashtariparviz efficientmethodforrecoveryoftargetssdnabasedonaminomodifiedsilicacoatedmagneticnanoparticles AT hexiaoxiao efficientmethodforrecoveryoftargetssdnabasedonaminomodifiedsilicacoatedmagneticnanoparticles AT wangkemin efficientmethodforrecoveryoftargetssdnabasedonaminomodifiedsilicacoatedmagneticnanoparticles AT gongping efficientmethodforrecoveryoftargetssdnabasedonaminomodifiedsilicacoatedmagneticnanoparticles |