Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice

When developing inhaled medicines for respiratory diseases, such as chronic obstructive pulmonary disease, drugs need to be administered by pulmonary delivery to animals in non-clinical tests. Common methods require application of pressure during administration, and it may cause lung injury, so we f...

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Autores principales: Oiso, Yuki, Akita, Tomomi, Kato, Daiki, Yamashita, Chikamasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150831/
https://www.ncbi.nlm.nih.gov/pubmed/32106551
http://dx.doi.org/10.3390/pharmaceutics12030200
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author Oiso, Yuki
Akita, Tomomi
Kato, Daiki
Yamashita, Chikamasa
author_facet Oiso, Yuki
Akita, Tomomi
Kato, Daiki
Yamashita, Chikamasa
author_sort Oiso, Yuki
collection PubMed
description When developing inhaled medicines for respiratory diseases, such as chronic obstructive pulmonary disease, drugs need to be administered by pulmonary delivery to animals in non-clinical tests. Common methods require application of pressure during administration, and it may cause lung injury, so we focused on the inhalation of liquid medicines by mice themselves. This study aimed to evaluate a negative pressure method of pulmonary administration in mice by self-inhalation. First, to confirm the accuracy of delivery of liquid medicines into lungs and the potential for lung injury, Institute of Cancer Research (ICR) mice received methylene blue tetrahydrate or saline by the negative pressure method. We assessed drug distribution and usefulness of this method by administering porcine pancreatic elastase and all-trans-retinoic acid (ATRA) to mice. Consequently, we confirmed good distribution of the dye and no injury such as disruption of blood flow or destruction of alveoli in lungs of mice. Following production of the murine emphysema model, the mean linear intercept (Lm) was calculated as 78 ± 4 μm. Moreover, a significant therapeutic effect of administration of the ATRA was confirmed. These results suggest that this negative pressure method of administration may be useful for pulmonary administration in non-clinical tests.
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spelling pubmed-71508312020-04-20 Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice Oiso, Yuki Akita, Tomomi Kato, Daiki Yamashita, Chikamasa Pharmaceutics Article When developing inhaled medicines for respiratory diseases, such as chronic obstructive pulmonary disease, drugs need to be administered by pulmonary delivery to animals in non-clinical tests. Common methods require application of pressure during administration, and it may cause lung injury, so we focused on the inhalation of liquid medicines by mice themselves. This study aimed to evaluate a negative pressure method of pulmonary administration in mice by self-inhalation. First, to confirm the accuracy of delivery of liquid medicines into lungs and the potential for lung injury, Institute of Cancer Research (ICR) mice received methylene blue tetrahydrate or saline by the negative pressure method. We assessed drug distribution and usefulness of this method by administering porcine pancreatic elastase and all-trans-retinoic acid (ATRA) to mice. Consequently, we confirmed good distribution of the dye and no injury such as disruption of blood flow or destruction of alveoli in lungs of mice. Following production of the murine emphysema model, the mean linear intercept (Lm) was calculated as 78 ± 4 μm. Moreover, a significant therapeutic effect of administration of the ATRA was confirmed. These results suggest that this negative pressure method of administration may be useful for pulmonary administration in non-clinical tests. MDPI 2020-02-25 /pmc/articles/PMC7150831/ /pubmed/32106551 http://dx.doi.org/10.3390/pharmaceutics12030200 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oiso, Yuki
Akita, Tomomi
Kato, Daiki
Yamashita, Chikamasa
Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice
title Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice
title_full Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice
title_fullStr Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice
title_full_unstemmed Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice
title_short Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice
title_sort method for pulmonary administration using negative pressure generated by inspiration in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150831/
https://www.ncbi.nlm.nih.gov/pubmed/32106551
http://dx.doi.org/10.3390/pharmaceutics12030200
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