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Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses

Many viruses infect and replicate in their host via the intestinal tract, e.g. many picornaviruses, several coronaviruses and avian influenza viruses of waterfowl. To analyze infection of enterocytes is a challenging task as culture systems for differentiated intestinal epithelial cells are not read...

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Autores principales: Punyadarsaniya, Darsaniya, Winter, Christine, Mork, Ann-Kathrin, Amiri, Mahdi, Naim, Hassan Y., Rautenschlein, Silke, Herrler, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172049/
https://www.ncbi.nlm.nih.gov/pubmed/25445801
http://dx.doi.org/10.1016/j.jviromet.2014.10.015
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author Punyadarsaniya, Darsaniya
Winter, Christine
Mork, Ann-Kathrin
Amiri, Mahdi
Naim, Hassan Y.
Rautenschlein, Silke
Herrler, Georg
author_facet Punyadarsaniya, Darsaniya
Winter, Christine
Mork, Ann-Kathrin
Amiri, Mahdi
Naim, Hassan Y.
Rautenschlein, Silke
Herrler, Georg
author_sort Punyadarsaniya, Darsaniya
collection PubMed
description Many viruses infect and replicate in their host via the intestinal tract, e.g. many picornaviruses, several coronaviruses and avian influenza viruses of waterfowl. To analyze infection of enterocytes is a challenging task as culture systems for differentiated intestinal epithelial cells are not readily available and often have a life span that is too short for infection studies. Precision-cut intestinal slices (PCIS) from chicken embryos were prepared and shown that the epithelial cells lining the lumen of the intestine are viable for up to 4 days. Using lectin staining, it was demonstrated that α2,3-linked sialic acids, the preferred receptor determinants of avian influenza viruses, are present on the apical side of the epithelial cells. Furthermore, the epithelial cells (at the tips) of the villi were shown to be susceptible to infection by an avian influenza virus of the H9N2 subtype. This culture system will be useful to analyze virus infection of intestinal epithelial cells and it should be applicable also to the intestine of other species.
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spelling pubmed-71720492020-04-22 Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses Punyadarsaniya, Darsaniya Winter, Christine Mork, Ann-Kathrin Amiri, Mahdi Naim, Hassan Y. Rautenschlein, Silke Herrler, Georg J Virol Methods Article Many viruses infect and replicate in their host via the intestinal tract, e.g. many picornaviruses, several coronaviruses and avian influenza viruses of waterfowl. To analyze infection of enterocytes is a challenging task as culture systems for differentiated intestinal epithelial cells are not readily available and often have a life span that is too short for infection studies. Precision-cut intestinal slices (PCIS) from chicken embryos were prepared and shown that the epithelial cells lining the lumen of the intestine are viable for up to 4 days. Using lectin staining, it was demonstrated that α2,3-linked sialic acids, the preferred receptor determinants of avian influenza viruses, are present on the apical side of the epithelial cells. Furthermore, the epithelial cells (at the tips) of the villi were shown to be susceptible to infection by an avian influenza virus of the H9N2 subtype. This culture system will be useful to analyze virus infection of intestinal epithelial cells and it should be applicable also to the intestine of other species. Elsevier B.V. 2015-02 2014-11-12 /pmc/articles/PMC7172049/ /pubmed/25445801 http://dx.doi.org/10.1016/j.jviromet.2014.10.015 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Punyadarsaniya, Darsaniya
Winter, Christine
Mork, Ann-Kathrin
Amiri, Mahdi
Naim, Hassan Y.
Rautenschlein, Silke
Herrler, Georg
Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses
title Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses
title_full Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses
title_fullStr Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses
title_full_unstemmed Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses
title_short Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses
title_sort precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172049/
https://www.ncbi.nlm.nih.gov/pubmed/25445801
http://dx.doi.org/10.1016/j.jviromet.2014.10.015
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