Nanoparticles Enhanced Self-Driven Microfludic Biosensor

C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plas...

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Autores principales: Liu, Chunxiu, Xue, Ning, Cai, Haoyuan, Sun, Jianhai, Qi, Zhimei, Zhao, Peiyue, Xiong, Fei, Geng, Zhaoxin, Jiang, Liying, Li, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231021/
https://www.ncbi.nlm.nih.gov/pubmed/32230908
http://dx.doi.org/10.3390/mi11040350
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author Liu, Chunxiu
Xue, Ning
Cai, Haoyuan
Sun, Jianhai
Qi, Zhimei
Zhao, Peiyue
Xiong, Fei
Geng, Zhaoxin
Jiang, Liying
Li, Li
author_facet Liu, Chunxiu
Xue, Ning
Cai, Haoyuan
Sun, Jianhai
Qi, Zhimei
Zhao, Peiyue
Xiong, Fei
Geng, Zhaoxin
Jiang, Liying
Li, Li
author_sort Liu, Chunxiu
collection PubMed
description C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods.
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spelling pubmed-72310212020-05-22 Nanoparticles Enhanced Self-Driven Microfludic Biosensor Liu, Chunxiu Xue, Ning Cai, Haoyuan Sun, Jianhai Qi, Zhimei Zhao, Peiyue Xiong, Fei Geng, Zhaoxin Jiang, Liying Li, Li Micromachines (Basel) Article C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods. MDPI 2020-03-27 /pmc/articles/PMC7231021/ /pubmed/32230908 http://dx.doi.org/10.3390/mi11040350 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Chunxiu
Xue, Ning
Cai, Haoyuan
Sun, Jianhai
Qi, Zhimei
Zhao, Peiyue
Xiong, Fei
Geng, Zhaoxin
Jiang, Liying
Li, Li
Nanoparticles Enhanced Self-Driven Microfludic Biosensor
title Nanoparticles Enhanced Self-Driven Microfludic Biosensor
title_full Nanoparticles Enhanced Self-Driven Microfludic Biosensor
title_fullStr Nanoparticles Enhanced Self-Driven Microfludic Biosensor
title_full_unstemmed Nanoparticles Enhanced Self-Driven Microfludic Biosensor
title_short Nanoparticles Enhanced Self-Driven Microfludic Biosensor
title_sort nanoparticles enhanced self-driven microfludic biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231021/
https://www.ncbi.nlm.nih.gov/pubmed/32230908
http://dx.doi.org/10.3390/mi11040350
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