Nanoparticles Enhanced Self-Driven Microfludic Biosensor
C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plas...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231021/ https://www.ncbi.nlm.nih.gov/pubmed/32230908 http://dx.doi.org/10.3390/mi11040350 |
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author | Liu, Chunxiu Xue, Ning Cai, Haoyuan Sun, Jianhai Qi, Zhimei Zhao, Peiyue Xiong, Fei Geng, Zhaoxin Jiang, Liying Li, Li |
author_facet | Liu, Chunxiu Xue, Ning Cai, Haoyuan Sun, Jianhai Qi, Zhimei Zhao, Peiyue Xiong, Fei Geng, Zhaoxin Jiang, Liying Li, Li |
author_sort | Liu, Chunxiu |
collection | PubMed |
description | C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods. |
format | Online Article Text |
id | pubmed-7231021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72310212020-05-22 Nanoparticles Enhanced Self-Driven Microfludic Biosensor Liu, Chunxiu Xue, Ning Cai, Haoyuan Sun, Jianhai Qi, Zhimei Zhao, Peiyue Xiong, Fei Geng, Zhaoxin Jiang, Liying Li, Li Micromachines (Basel) Article C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods. MDPI 2020-03-27 /pmc/articles/PMC7231021/ /pubmed/32230908 http://dx.doi.org/10.3390/mi11040350 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Chunxiu Xue, Ning Cai, Haoyuan Sun, Jianhai Qi, Zhimei Zhao, Peiyue Xiong, Fei Geng, Zhaoxin Jiang, Liying Li, Li Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
title | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
title_full | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
title_fullStr | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
title_full_unstemmed | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
title_short | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
title_sort | nanoparticles enhanced self-driven microfludic biosensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231021/ https://www.ncbi.nlm.nih.gov/pubmed/32230908 http://dx.doi.org/10.3390/mi11040350 |
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