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Low Turnover of Soil Bacterial rRNA at Low Temperatures

Ribosomal RNA (rRNA) is used widely to investigate potentially active microorganisms in environmental samples, including soil microorganisms and other microbial communities that are subjected to pronounced seasonal variation in temperature. This raises a question about the turnover of intracellular...

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Autores principales: Schostag, Morten Dencker, Albers, Christian Nyrop, Jacobsen, Carsten Suhr, Priemé, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261852/
https://www.ncbi.nlm.nih.gov/pubmed/32523564
http://dx.doi.org/10.3389/fmicb.2020.00962
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author Schostag, Morten Dencker
Albers, Christian Nyrop
Jacobsen, Carsten Suhr
Priemé, Anders
author_facet Schostag, Morten Dencker
Albers, Christian Nyrop
Jacobsen, Carsten Suhr
Priemé, Anders
author_sort Schostag, Morten Dencker
collection PubMed
description Ribosomal RNA (rRNA) is used widely to investigate potentially active microorganisms in environmental samples, including soil microorganisms and other microbial communities that are subjected to pronounced seasonal variation in temperature. This raises a question about the turnover of intracellular microbial rRNA at environmentally relevant temperatures. We analyzed the turnover at four temperatures of RNA isolated from soil bacteria amended with (14)C-labeled uridine. We found that the half-life of recently produced RNA increased from 4.0 days at 20°C to 15.8 days at 4°C, and 215 days at −4°C, while no degradation was detected at −18°C during a 1-year period. We discuss the implications of the strong temperature dependency of rRNA turnover for interpretation of microbiome data based on rRNA isolated from environmental samples.
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spelling pubmed-72618522020-06-09 Low Turnover of Soil Bacterial rRNA at Low Temperatures Schostag, Morten Dencker Albers, Christian Nyrop Jacobsen, Carsten Suhr Priemé, Anders Front Microbiol Microbiology Ribosomal RNA (rRNA) is used widely to investigate potentially active microorganisms in environmental samples, including soil microorganisms and other microbial communities that are subjected to pronounced seasonal variation in temperature. This raises a question about the turnover of intracellular microbial rRNA at environmentally relevant temperatures. We analyzed the turnover at four temperatures of RNA isolated from soil bacteria amended with (14)C-labeled uridine. We found that the half-life of recently produced RNA increased from 4.0 days at 20°C to 15.8 days at 4°C, and 215 days at −4°C, while no degradation was detected at −18°C during a 1-year period. We discuss the implications of the strong temperature dependency of rRNA turnover for interpretation of microbiome data based on rRNA isolated from environmental samples. Frontiers Media S.A. 2020-05-25 /pmc/articles/PMC7261852/ /pubmed/32523564 http://dx.doi.org/10.3389/fmicb.2020.00962 Text en Copyright © 2020 Schostag, Albers, Jacobsen and Priemé. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Schostag, Morten Dencker
Albers, Christian Nyrop
Jacobsen, Carsten Suhr
Priemé, Anders
Low Turnover of Soil Bacterial rRNA at Low Temperatures
title Low Turnover of Soil Bacterial rRNA at Low Temperatures
title_full Low Turnover of Soil Bacterial rRNA at Low Temperatures
title_fullStr Low Turnover of Soil Bacterial rRNA at Low Temperatures
title_full_unstemmed Low Turnover of Soil Bacterial rRNA at Low Temperatures
title_short Low Turnover of Soil Bacterial rRNA at Low Temperatures
title_sort low turnover of soil bacterial rrna at low temperatures
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261852/
https://www.ncbi.nlm.nih.gov/pubmed/32523564
http://dx.doi.org/10.3389/fmicb.2020.00962
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