Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels

Mechanotransduction channels have been proposed as force sensors in various physiological processes, such as hearing and touch. In particular, TMC1 has been shown to constitute the pore of hair cell mechanotransduction channels, but little is known about how force is sensed by TMC channels. Here, we...

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Autores principales: Tang, Yi-Quan, Lee, Sol Ah, Rahman, Mizanur, Vanapalli, Siva A., Lu, Hang, Schafer, William R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343241/
https://www.ncbi.nlm.nih.gov/pubmed/32325031
http://dx.doi.org/10.1016/j.neuron.2020.03.026
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author Tang, Yi-Quan
Lee, Sol Ah
Rahman, Mizanur
Vanapalli, Siva A.
Lu, Hang
Schafer, William R.
author_facet Tang, Yi-Quan
Lee, Sol Ah
Rahman, Mizanur
Vanapalli, Siva A.
Lu, Hang
Schafer, William R.
author_sort Tang, Yi-Quan
collection PubMed
description Mechanotransduction channels have been proposed as force sensors in various physiological processes, such as hearing and touch. In particular, TMC1 has been shown to constitute the pore of hair cell mechanotransduction channels, but little is known about how force is sensed by TMC channels. Here, we identify UNC-44/ankyrin as an essential component of the TMC-1 mechanotransduction channel complex in the sensory cilia of Caenorhabditis elegans mechanoreceptor neurons. Ankyrin binds indirectly to TMC-1 via evolutionarily conserved CIB proteins, which are required for TMC-1-mediated mechanosensation in C. elegans OLQ neurons and body wall muscles. Mechanosensory activity conferred by ectopically expressed TMCs in mechanoinsensitive neurons depends on both ankyrin and CIB proteins, indicating that the ankyrin-CIB subcomplex is required for TMC mechanosensitivity. Our work indicates that ankyrin is a long-sought intracellular tether that transmits force to TMC mechanotransduction channels.
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spelling pubmed-73432412020-07-14 Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels Tang, Yi-Quan Lee, Sol Ah Rahman, Mizanur Vanapalli, Siva A. Lu, Hang Schafer, William R. Neuron Article Mechanotransduction channels have been proposed as force sensors in various physiological processes, such as hearing and touch. In particular, TMC1 has been shown to constitute the pore of hair cell mechanotransduction channels, but little is known about how force is sensed by TMC channels. Here, we identify UNC-44/ankyrin as an essential component of the TMC-1 mechanotransduction channel complex in the sensory cilia of Caenorhabditis elegans mechanoreceptor neurons. Ankyrin binds indirectly to TMC-1 via evolutionarily conserved CIB proteins, which are required for TMC-1-mediated mechanosensation in C. elegans OLQ neurons and body wall muscles. Mechanosensory activity conferred by ectopically expressed TMCs in mechanoinsensitive neurons depends on both ankyrin and CIB proteins, indicating that the ankyrin-CIB subcomplex is required for TMC mechanosensitivity. Our work indicates that ankyrin is a long-sought intracellular tether that transmits force to TMC mechanotransduction channels. Cell Press 2020-07-08 /pmc/articles/PMC7343241/ /pubmed/32325031 http://dx.doi.org/10.1016/j.neuron.2020.03.026 Text en © 2020 MRC Laboratory of Molecular Biology http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Yi-Quan
Lee, Sol Ah
Rahman, Mizanur
Vanapalli, Siva A.
Lu, Hang
Schafer, William R.
Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels
title Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels
title_full Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels
title_fullStr Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels
title_full_unstemmed Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels
title_short Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels
title_sort ankyrin is an intracellular tether for tmc mechanotransduction channels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343241/
https://www.ncbi.nlm.nih.gov/pubmed/32325031
http://dx.doi.org/10.1016/j.neuron.2020.03.026
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