Efficient inactivation of African swine fever virus by ozonized water

African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), which is a devastating disease of domestic pigs and wild boar, causing significant economic losses to the pig industry worldwide. To evaluate the ability of ozonized water as a disinfectant to inactivate ASFV, ozoni...

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Autores principales: Zhang, Li, Luo, Yuzi, Wang, Wei, Sun, Yuan, Zhang, Jiaoer, Fatima, Madiha, Jia, Xiangdong, Qiu, Hua-Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348602/
https://www.ncbi.nlm.nih.gov/pubmed/32768237
http://dx.doi.org/10.1016/j.vetmic.2020.108796
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author Zhang, Li
Luo, Yuzi
Wang, Wei
Sun, Yuan
Zhang, Jiaoer
Fatima, Madiha
Jia, Xiangdong
Qiu, Hua-Ji
author_facet Zhang, Li
Luo, Yuzi
Wang, Wei
Sun, Yuan
Zhang, Jiaoer
Fatima, Madiha
Jia, Xiangdong
Qiu, Hua-Ji
author_sort Zhang, Li
collection PubMed
description African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), which is a devastating disease of domestic pigs and wild boar, causing significant economic losses to the pig industry worldwide. To evaluate the ability of ozonized water as a disinfectant to inactivate ASFV, ozonized water of different concentrations was tested, and the viral reduction was determined by infectivity assay on porcine primary alveolar macrophages. The results showed that 2 log10 (99 %) reduction in viral titer was observed when 10(4.0) TCID(50)/mL wild-type or reporter ASFV was inactivated with ozonized water as lower as 5 mg/L within 1 min at room temperature; while a viral reduction of approximately 2 log10 (99 %) was observed when 10(5.0) TCID(50)/mL wild-type or reporter ASFV was inactivated with 5 mg/L ozonized water within 1 min, and 3 log10 (99.9 %) virus was inactivated by 10 or 20 mg/L ozonized water within 3 or 1 min, respectively; furthermore, 5 mg/L ozonized water inactivated 2 log10 (99 %) reporter ASFV as higher as 10(6.75) TCID(50)/mL in 1 min, and a viral reduction of approximately 3 log10 (99.9 %) in reporter ASFV or 2 log10 (99 %) in wild-type virus was observed when inactivated with 10 mg/L ozonized water in 1 min; meanwhile, a viral reduction of 3 log10 (99.9 %) was observed when 20 mg/L ozonized water was applied to the wild-type ASFV of 10(6.75) TCID(50)/mL in 3 min. Overall, ozonized water can rapidly and efficiently inactivate ASFV, representing an effective disinfectant for ASF control.
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spelling pubmed-73486022020-07-10 Efficient inactivation of African swine fever virus by ozonized water Zhang, Li Luo, Yuzi Wang, Wei Sun, Yuan Zhang, Jiaoer Fatima, Madiha Jia, Xiangdong Qiu, Hua-Ji Vet Microbiol Article African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), which is a devastating disease of domestic pigs and wild boar, causing significant economic losses to the pig industry worldwide. To evaluate the ability of ozonized water as a disinfectant to inactivate ASFV, ozonized water of different concentrations was tested, and the viral reduction was determined by infectivity assay on porcine primary alveolar macrophages. The results showed that 2 log10 (99 %) reduction in viral titer was observed when 10(4.0) TCID(50)/mL wild-type or reporter ASFV was inactivated with ozonized water as lower as 5 mg/L within 1 min at room temperature; while a viral reduction of approximately 2 log10 (99 %) was observed when 10(5.0) TCID(50)/mL wild-type or reporter ASFV was inactivated with 5 mg/L ozonized water within 1 min, and 3 log10 (99.9 %) virus was inactivated by 10 or 20 mg/L ozonized water within 3 or 1 min, respectively; furthermore, 5 mg/L ozonized water inactivated 2 log10 (99 %) reporter ASFV as higher as 10(6.75) TCID(50)/mL in 1 min, and a viral reduction of approximately 3 log10 (99.9 %) in reporter ASFV or 2 log10 (99 %) in wild-type virus was observed when inactivated with 10 mg/L ozonized water in 1 min; meanwhile, a viral reduction of 3 log10 (99.9 %) was observed when 20 mg/L ozonized water was applied to the wild-type ASFV of 10(6.75) TCID(50)/mL in 3 min. Overall, ozonized water can rapidly and efficiently inactivate ASFV, representing an effective disinfectant for ASF control. Elsevier B.V. 2020-08 2020-07-10 /pmc/articles/PMC7348602/ /pubmed/32768237 http://dx.doi.org/10.1016/j.vetmic.2020.108796 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhang, Li
Luo, Yuzi
Wang, Wei
Sun, Yuan
Zhang, Jiaoer
Fatima, Madiha
Jia, Xiangdong
Qiu, Hua-Ji
Efficient inactivation of African swine fever virus by ozonized water
title Efficient inactivation of African swine fever virus by ozonized water
title_full Efficient inactivation of African swine fever virus by ozonized water
title_fullStr Efficient inactivation of African swine fever virus by ozonized water
title_full_unstemmed Efficient inactivation of African swine fever virus by ozonized water
title_short Efficient inactivation of African swine fever virus by ozonized water
title_sort efficient inactivation of african swine fever virus by ozonized water
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348602/
https://www.ncbi.nlm.nih.gov/pubmed/32768237
http://dx.doi.org/10.1016/j.vetmic.2020.108796
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