Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity
Human dendritic cells (DCs) comprise subsets with distinct phenotypic and functional characteristics, but the transcriptional programs that dictate their identity remain elusive. Here, we analyze global chromatin accessibility profiles across resting and stimulated human DC subsets by means of the a...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546547/ https://www.ncbi.nlm.nih.gov/pubmed/32966789 http://dx.doi.org/10.1016/j.celrep.2020.108180 |
_version_ | 1783592247477927936 |
---|---|
author | Leylek, Rebecca Alcántara-Hernández, Marcela Granja, Jeffrey M. Chavez, Michael Perez, Kimberly Diaz, Oscar R. Li, Rui Satpathy, Ansuman T. Chang, Howard Y. Idoyaga, Juliana |
author_facet | Leylek, Rebecca Alcántara-Hernández, Marcela Granja, Jeffrey M. Chavez, Michael Perez, Kimberly Diaz, Oscar R. Li, Rui Satpathy, Ansuman T. Chang, Howard Y. Idoyaga, Juliana |
author_sort | Leylek, Rebecca |
collection | PubMed |
description | Human dendritic cells (DCs) comprise subsets with distinct phenotypic and functional characteristics, but the transcriptional programs that dictate their identity remain elusive. Here, we analyze global chromatin accessibility profiles across resting and stimulated human DC subsets by means of the assay for transposase-accessible chromatin using sequencing (ATAC-seq). We uncover specific regions of chromatin accessibility for each subset and transcriptional regulators of DC function. By comparing plasmacytoid DC responses to IFN-I-producing and non-IFN-I-producing conditions, we identify genetic programs related to their function. Finally, by intersecting chromatin accessibility with genome-wide association studies, we recognize DC subset-specific enrichment of heritability in autoimmune diseases. Our results unravel the basis of human DC subset heterogeneity and provide a framework for their analysis in disease pathogenesis. |
format | Online Article Text |
id | pubmed-7546547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-75465472020-10-09 Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity Leylek, Rebecca Alcántara-Hernández, Marcela Granja, Jeffrey M. Chavez, Michael Perez, Kimberly Diaz, Oscar R. Li, Rui Satpathy, Ansuman T. Chang, Howard Y. Idoyaga, Juliana Cell Rep Article Human dendritic cells (DCs) comprise subsets with distinct phenotypic and functional characteristics, but the transcriptional programs that dictate their identity remain elusive. Here, we analyze global chromatin accessibility profiles across resting and stimulated human DC subsets by means of the assay for transposase-accessible chromatin using sequencing (ATAC-seq). We uncover specific regions of chromatin accessibility for each subset and transcriptional regulators of DC function. By comparing plasmacytoid DC responses to IFN-I-producing and non-IFN-I-producing conditions, we identify genetic programs related to their function. Finally, by intersecting chromatin accessibility with genome-wide association studies, we recognize DC subset-specific enrichment of heritability in autoimmune diseases. Our results unravel the basis of human DC subset heterogeneity and provide a framework for their analysis in disease pathogenesis. 2020-09-22 /pmc/articles/PMC7546547/ /pubmed/32966789 http://dx.doi.org/10.1016/j.celrep.2020.108180 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Leylek, Rebecca Alcántara-Hernández, Marcela Granja, Jeffrey M. Chavez, Michael Perez, Kimberly Diaz, Oscar R. Li, Rui Satpathy, Ansuman T. Chang, Howard Y. Idoyaga, Juliana Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity |
title | Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity |
title_full | Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity |
title_fullStr | Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity |
title_full_unstemmed | Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity |
title_short | Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity |
title_sort | chromatin landscape underpinning human dendritic cell heterogeneity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546547/ https://www.ncbi.nlm.nih.gov/pubmed/32966789 http://dx.doi.org/10.1016/j.celrep.2020.108180 |
work_keys_str_mv | AT leylekrebecca chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT alcantarahernandezmarcela chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT granjajeffreym chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT chavezmichael chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT perezkimberly chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT diazoscarr chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT lirui chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT satpathyansumant chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT changhowardy chromatinlandscapeunderpinninghumandendriticcellheterogeneity AT idoyagajuliana chromatinlandscapeunderpinninghumandendriticcellheterogeneity |