GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway

This paper aimed to research the function and in-depth mechanism of GPR37 in lung adenocarcinoma (LUAD). Herein, based on TCGA and Oncomine databases, we revealed that GPR37 was expressed at high levels in LUAD, and upregulation of GPR37 was related to the poor outcomes. Furthermore, biological func...

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Autores principales: Wang, Jian, Xu, Min, Li, Dan-Dan, Abudukelimu, Wujikenayi, Zhou, Xiu-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730288/
https://www.ncbi.nlm.nih.gov/pubmed/33364431
http://dx.doi.org/10.1515/med-2021-0011
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author Wang, Jian
Xu, Min
Li, Dan-Dan
Abudukelimu, Wujikenayi
Zhou, Xiu-Hong
author_facet Wang, Jian
Xu, Min
Li, Dan-Dan
Abudukelimu, Wujikenayi
Zhou, Xiu-Hong
author_sort Wang, Jian
collection PubMed
description This paper aimed to research the function and in-depth mechanism of GPR37 in lung adenocarcinoma (LUAD). Herein, based on TCGA and Oncomine databases, we revealed that GPR37 was expressed at high levels in LUAD, and upregulation of GPR37 was related to the poor outcomes. Furthermore, biological function experiments in vitro were utilized to assess whether GPR37 impacts malignant phenotype of LUAD cells. Gain- or loss-of-function assays indicated that the upregulation of GPR37 contributed to improving the proliferation, migration, and invasion of LUAD cells in vitro, while knockdown of GPR37 can inhibit the malignant biological behaviors. Then, we found that depletion of GPR37 resulted in a decrease in the expression of TGF-β1 as well as the extents of Smad2 and Smad3 phosphorylation, while overexpression of GPR37 presented opposite outcomes. Altogether, our findings indicated that GPR37 is a potential oncogene of LUAD, and its promoting effects on the malignant progression of LUAD may be realized via TGF-β/Smad pathway.
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spelling pubmed-77302882020-12-23 GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway Wang, Jian Xu, Min Li, Dan-Dan Abudukelimu, Wujikenayi Zhou, Xiu-Hong Open Med (Wars) Research Article This paper aimed to research the function and in-depth mechanism of GPR37 in lung adenocarcinoma (LUAD). Herein, based on TCGA and Oncomine databases, we revealed that GPR37 was expressed at high levels in LUAD, and upregulation of GPR37 was related to the poor outcomes. Furthermore, biological function experiments in vitro were utilized to assess whether GPR37 impacts malignant phenotype of LUAD cells. Gain- or loss-of-function assays indicated that the upregulation of GPR37 contributed to improving the proliferation, migration, and invasion of LUAD cells in vitro, while knockdown of GPR37 can inhibit the malignant biological behaviors. Then, we found that depletion of GPR37 resulted in a decrease in the expression of TGF-β1 as well as the extents of Smad2 and Smad3 phosphorylation, while overexpression of GPR37 presented opposite outcomes. Altogether, our findings indicated that GPR37 is a potential oncogene of LUAD, and its promoting effects on the malignant progression of LUAD may be realized via TGF-β/Smad pathway. De Gruyter 2020-12-05 /pmc/articles/PMC7730288/ /pubmed/33364431 http://dx.doi.org/10.1515/med-2021-0011 Text en © 2021 Jian Wang et al., published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Wang, Jian
Xu, Min
Li, Dan-Dan
Abudukelimu, Wujikenayi
Zhou, Xiu-Hong
GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway
title GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway
title_full GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway
title_fullStr GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway
title_full_unstemmed GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway
title_short GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway
title_sort gpr37 promotes the malignancy of lung adenocarcinoma via tgf-β/smad pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730288/
https://www.ncbi.nlm.nih.gov/pubmed/33364431
http://dx.doi.org/10.1515/med-2021-0011
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