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Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1

Ring finger protein 2 (RNF2) is an important component of polycomb repressive complex 1. RNF2 is upregulated in many kinds of tumors, and elevated RNF2 expression is associated with a poor prognosis in certain cancers. To assess the function of RNF2 in colorectal cancer, we examined RNF2 protein lev...

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Autores principales: Wei, Feilong, Jing, Haoren, Wei, Ming, Liu, Lei, Wu, Jieheng, Wang, Meng, Han, Donghui, Yang, Fa, Yang, Bo, Jiao, Dian, Zheng, Guoxu, Zhang, Lingling, Xi, Wenjin, Guo, Zhangyan, Yang, An-Gang, Qin, Weijun, Zhou, Yi, Wen, Weihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803491/
https://www.ncbi.nlm.nih.gov/pubmed/33346749
http://dx.doi.org/10.18632/aging.202396
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author Wei, Feilong
Jing, Haoren
Wei, Ming
Liu, Lei
Wu, Jieheng
Wang, Meng
Han, Donghui
Yang, Fa
Yang, Bo
Jiao, Dian
Zheng, Guoxu
Zhang, Lingling
Xi, Wenjin
Guo, Zhangyan
Yang, An-Gang
Qin, Weijun
Zhou, Yi
Wen, Weihong
author_facet Wei, Feilong
Jing, Haoren
Wei, Ming
Liu, Lei
Wu, Jieheng
Wang, Meng
Han, Donghui
Yang, Fa
Yang, Bo
Jiao, Dian
Zheng, Guoxu
Zhang, Lingling
Xi, Wenjin
Guo, Zhangyan
Yang, An-Gang
Qin, Weijun
Zhou, Yi
Wen, Weihong
author_sort Wei, Feilong
collection PubMed
description Ring finger protein 2 (RNF2) is an important component of polycomb repressive complex 1. RNF2 is upregulated in many kinds of tumors, and elevated RNF2 expression is associated with a poor prognosis in certain cancers. To assess the function of RNF2 in colorectal cancer, we examined RNF2 protein levels in 313 paired colorectal cancer tissues and adjacent normal tissues. We then analyzed the association of RNF2 expression with the patients’ clinicopathologic features and prognoses. RNF2 expression was upregulated in colorectal cancer tissues and was associated with the tumor differentiation status, tumor stage and prognosis. In colorectal cancer cell lines, downregulation of RNF2 inhibited cell proliferation and induced apoptosis. Gene microarray analysis revealed that early growth response 1 (EGR1) was upregulated in RNF2-knockdown cells. Knocking down EGR1 partially reversed the inhibition of cell proliferation and the induction of apoptosis in RNF2-knockdown cells. RNF2 was enriched at the EGR1 promoter, where it mono-ubiquitinated histone H2A, thereby inhibiting EGR1 expression. These results indicate that RNF2 is oncogenic in colorectal cancer and may promote disease progression by inhibiting EGR1 expression. RNF2 is thus a potential prognostic marker and therapeutic target in colorectal cancer.
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spelling pubmed-78034912021-01-15 Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1 Wei, Feilong Jing, Haoren Wei, Ming Liu, Lei Wu, Jieheng Wang, Meng Han, Donghui Yang, Fa Yang, Bo Jiao, Dian Zheng, Guoxu Zhang, Lingling Xi, Wenjin Guo, Zhangyan Yang, An-Gang Qin, Weijun Zhou, Yi Wen, Weihong Aging (Albany NY) Research Paper Ring finger protein 2 (RNF2) is an important component of polycomb repressive complex 1. RNF2 is upregulated in many kinds of tumors, and elevated RNF2 expression is associated with a poor prognosis in certain cancers. To assess the function of RNF2 in colorectal cancer, we examined RNF2 protein levels in 313 paired colorectal cancer tissues and adjacent normal tissues. We then analyzed the association of RNF2 expression with the patients’ clinicopathologic features and prognoses. RNF2 expression was upregulated in colorectal cancer tissues and was associated with the tumor differentiation status, tumor stage and prognosis. In colorectal cancer cell lines, downregulation of RNF2 inhibited cell proliferation and induced apoptosis. Gene microarray analysis revealed that early growth response 1 (EGR1) was upregulated in RNF2-knockdown cells. Knocking down EGR1 partially reversed the inhibition of cell proliferation and the induction of apoptosis in RNF2-knockdown cells. RNF2 was enriched at the EGR1 promoter, where it mono-ubiquitinated histone H2A, thereby inhibiting EGR1 expression. These results indicate that RNF2 is oncogenic in colorectal cancer and may promote disease progression by inhibiting EGR1 expression. RNF2 is thus a potential prognostic marker and therapeutic target in colorectal cancer. Impact Journals 2020-12-19 /pmc/articles/PMC7803491/ /pubmed/33346749 http://dx.doi.org/10.18632/aging.202396 Text en Copyright: © 2020 Wei et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wei, Feilong
Jing, Haoren
Wei, Ming
Liu, Lei
Wu, Jieheng
Wang, Meng
Han, Donghui
Yang, Fa
Yang, Bo
Jiao, Dian
Zheng, Guoxu
Zhang, Lingling
Xi, Wenjin
Guo, Zhangyan
Yang, An-Gang
Qin, Weijun
Zhou, Yi
Wen, Weihong
Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1
title Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1
title_full Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1
title_fullStr Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1
title_full_unstemmed Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1
title_short Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1
title_sort ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803491/
https://www.ncbi.nlm.nih.gov/pubmed/33346749
http://dx.doi.org/10.18632/aging.202396
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