Autophagy Mediates Leptin-Induced Migration and ERK Activation in Breast Cancer Cells

Autophagy is an intracellular recycling process active in eukaryotic cells that involves the formation of an autophagosome which delivers cytoplasmic components to the lysosome for degradation. This process occurs at low rates under basal conditions, but it can be induced by diverse types of stress...

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Autores principales: García-Miranda, Alin, Solano-Alcalá, Karen Aylín, Montes-Alvarado, José Benito, Rosas-Cruz, Arely, Reyes-Leyva, Julio, Navarro-Tito, Napoleón, Maycotte, Paola, Castañeda-Saucedo, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982408/
https://www.ncbi.nlm.nih.gov/pubmed/33763424
http://dx.doi.org/10.3389/fcell.2021.644851
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author García-Miranda, Alin
Solano-Alcalá, Karen Aylín
Montes-Alvarado, José Benito
Rosas-Cruz, Arely
Reyes-Leyva, Julio
Navarro-Tito, Napoleón
Maycotte, Paola
Castañeda-Saucedo, Eduardo
author_facet García-Miranda, Alin
Solano-Alcalá, Karen Aylín
Montes-Alvarado, José Benito
Rosas-Cruz, Arely
Reyes-Leyva, Julio
Navarro-Tito, Napoleón
Maycotte, Paola
Castañeda-Saucedo, Eduardo
author_sort García-Miranda, Alin
collection PubMed
description Autophagy is an intracellular recycling process active in eukaryotic cells that involves the formation of an autophagosome which delivers cytoplasmic components to the lysosome for degradation. This process occurs at low rates under basal conditions, but it can be induced by diverse types of stress such as starvation, hypoxia, metabolic disorders or in response to hormones, including leptin. Leptin is considered a pro-tumorigenic protein whose circulating levels have been related to bad prognosis in obese breast cancer patients. It has been recently demonstrated that leptin can induce autophagy in cancer cell lines from different tissues, suggesting that autophagy could modulate the pro-tumorigenic effects associated with leptin. In this study, the role of autophagy in leptin-induced proliferation, migration, apoptosis and ERK phosphorylation in breast cancer cell lines was evaluated. Although leptin differentially induced autophagy in the breast cancer cell lines tested, autophagy inhibition reduced leptin-induced cell proliferation in MCF7 cells and decreased cell migration, ERK activation, and impaired morphological changes in both cell lines. Our data demonstrates an important role for basal autophagy or leptin-induced autophagy in leptin-induced migration and ERK phosphorylation in breast cancer cell lines, suggesting a potential use for the inhibition of autophagy in breast cancer associated with obesity.
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spelling pubmed-79824082021-03-23 Autophagy Mediates Leptin-Induced Migration and ERK Activation in Breast Cancer Cells García-Miranda, Alin Solano-Alcalá, Karen Aylín Montes-Alvarado, José Benito Rosas-Cruz, Arely Reyes-Leyva, Julio Navarro-Tito, Napoleón Maycotte, Paola Castañeda-Saucedo, Eduardo Front Cell Dev Biol Cell and Developmental Biology Autophagy is an intracellular recycling process active in eukaryotic cells that involves the formation of an autophagosome which delivers cytoplasmic components to the lysosome for degradation. This process occurs at low rates under basal conditions, but it can be induced by diverse types of stress such as starvation, hypoxia, metabolic disorders or in response to hormones, including leptin. Leptin is considered a pro-tumorigenic protein whose circulating levels have been related to bad prognosis in obese breast cancer patients. It has been recently demonstrated that leptin can induce autophagy in cancer cell lines from different tissues, suggesting that autophagy could modulate the pro-tumorigenic effects associated with leptin. In this study, the role of autophagy in leptin-induced proliferation, migration, apoptosis and ERK phosphorylation in breast cancer cell lines was evaluated. Although leptin differentially induced autophagy in the breast cancer cell lines tested, autophagy inhibition reduced leptin-induced cell proliferation in MCF7 cells and decreased cell migration, ERK activation, and impaired morphological changes in both cell lines. Our data demonstrates an important role for basal autophagy or leptin-induced autophagy in leptin-induced migration and ERK phosphorylation in breast cancer cell lines, suggesting a potential use for the inhibition of autophagy in breast cancer associated with obesity. Frontiers Media S.A. 2021-03-08 /pmc/articles/PMC7982408/ /pubmed/33763424 http://dx.doi.org/10.3389/fcell.2021.644851 Text en Copyright © 2021 García-Miranda, Solano-Alcalá, Montes-Alvarado, Rosas-Cruz, Reyes-Leyva, Navarro-Tito, Maycotte and Castañeda-Saucedo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
García-Miranda, Alin
Solano-Alcalá, Karen Aylín
Montes-Alvarado, José Benito
Rosas-Cruz, Arely
Reyes-Leyva, Julio
Navarro-Tito, Napoleón
Maycotte, Paola
Castañeda-Saucedo, Eduardo
Autophagy Mediates Leptin-Induced Migration and ERK Activation in Breast Cancer Cells
title Autophagy Mediates Leptin-Induced Migration and ERK Activation in Breast Cancer Cells
title_full Autophagy Mediates Leptin-Induced Migration and ERK Activation in Breast Cancer Cells
title_fullStr Autophagy Mediates Leptin-Induced Migration and ERK Activation in Breast Cancer Cells
title_full_unstemmed Autophagy Mediates Leptin-Induced Migration and ERK Activation in Breast Cancer Cells
title_short Autophagy Mediates Leptin-Induced Migration and ERK Activation in Breast Cancer Cells
title_sort autophagy mediates leptin-induced migration and erk activation in breast cancer cells
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982408/
https://www.ncbi.nlm.nih.gov/pubmed/33763424
http://dx.doi.org/10.3389/fcell.2021.644851
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