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Targeted DNA methylation profiling reveals epigenetic signatures in peanut allergy

DNA methylation (DNAm) has been shown to play a role in mediating food allergy; however, the mechanism by which it does so is poorly understood. In this study, we used targeted next-generation bisulfite sequencing to evaluate DNAm levels in 125 targeted highly informative genomic regions containing...

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Autores principales: Zhou, Xiaoying, Han, Xiaorui, Lyu, Shu-Chen, Bunning, Bryan, Kost, Laurie, Chang, Iris, Cao, Shu, Sampath, Vanitha, Nadeau, Kari C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Clinical Investigation 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026193/
https://www.ncbi.nlm.nih.gov/pubmed/33571165
http://dx.doi.org/10.1172/jci.insight.143058
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author Zhou, Xiaoying
Han, Xiaorui
Lyu, Shu-Chen
Bunning, Bryan
Kost, Laurie
Chang, Iris
Cao, Shu
Sampath, Vanitha
Nadeau, Kari C.
author_facet Zhou, Xiaoying
Han, Xiaorui
Lyu, Shu-Chen
Bunning, Bryan
Kost, Laurie
Chang, Iris
Cao, Shu
Sampath, Vanitha
Nadeau, Kari C.
author_sort Zhou, Xiaoying
collection PubMed
description DNA methylation (DNAm) has been shown to play a role in mediating food allergy; however, the mechanism by which it does so is poorly understood. In this study, we used targeted next-generation bisulfite sequencing to evaluate DNAm levels in 125 targeted highly informative genomic regions containing 602 CpG sites on 70 immune-related genes to understand whether DNAm can differentiate peanut allergy (PA) versus nonallergy (NA). We found PA-associated DNAm signatures associated with 12 genes (7 potentially novel to food allergy, 3 associated with Th1/Th2, and 2 associated with innate immunity), as well as DNAm signature combinations with superior diagnostic potential compared with serum peanut–specific IgE for PA versus NA. Furthermore, we found that, following peanut protein stimulation, peripheral blood mononuclear cell (PBMCs) from PA participants showed increased production of cognate cytokines compared with NA participants. The varying responses between PA and NA participants may be associated with the interaction between the modification of DNAm and the interference of environment. Using Euclidean distance analysis, we found that the distances of methylation profile comprising 12 DNAm signatures between PA and NA pairs in monozygotic (MZ) twins were smaller than those in randomly paired genetically unrelated individuals, suggesting that PA-related DNAm signatures may be associated with genetic factors.
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spelling pubmed-80261932021-04-13 Targeted DNA methylation profiling reveals epigenetic signatures in peanut allergy Zhou, Xiaoying Han, Xiaorui Lyu, Shu-Chen Bunning, Bryan Kost, Laurie Chang, Iris Cao, Shu Sampath, Vanitha Nadeau, Kari C. JCI Insight Research Article DNA methylation (DNAm) has been shown to play a role in mediating food allergy; however, the mechanism by which it does so is poorly understood. In this study, we used targeted next-generation bisulfite sequencing to evaluate DNAm levels in 125 targeted highly informative genomic regions containing 602 CpG sites on 70 immune-related genes to understand whether DNAm can differentiate peanut allergy (PA) versus nonallergy (NA). We found PA-associated DNAm signatures associated with 12 genes (7 potentially novel to food allergy, 3 associated with Th1/Th2, and 2 associated with innate immunity), as well as DNAm signature combinations with superior diagnostic potential compared with serum peanut–specific IgE for PA versus NA. Furthermore, we found that, following peanut protein stimulation, peripheral blood mononuclear cell (PBMCs) from PA participants showed increased production of cognate cytokines compared with NA participants. The varying responses between PA and NA participants may be associated with the interaction between the modification of DNAm and the interference of environment. Using Euclidean distance analysis, we found that the distances of methylation profile comprising 12 DNAm signatures between PA and NA pairs in monozygotic (MZ) twins were smaller than those in randomly paired genetically unrelated individuals, suggesting that PA-related DNAm signatures may be associated with genetic factors. American Society for Clinical Investigation 2021-03-22 /pmc/articles/PMC8026193/ /pubmed/33571165 http://dx.doi.org/10.1172/jci.insight.143058 Text en © 2021 Zhou et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Zhou, Xiaoying
Han, Xiaorui
Lyu, Shu-Chen
Bunning, Bryan
Kost, Laurie
Chang, Iris
Cao, Shu
Sampath, Vanitha
Nadeau, Kari C.
Targeted DNA methylation profiling reveals epigenetic signatures in peanut allergy
title Targeted DNA methylation profiling reveals epigenetic signatures in peanut allergy
title_full Targeted DNA methylation profiling reveals epigenetic signatures in peanut allergy
title_fullStr Targeted DNA methylation profiling reveals epigenetic signatures in peanut allergy
title_full_unstemmed Targeted DNA methylation profiling reveals epigenetic signatures in peanut allergy
title_short Targeted DNA methylation profiling reveals epigenetic signatures in peanut allergy
title_sort targeted dna methylation profiling reveals epigenetic signatures in peanut allergy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026193/
https://www.ncbi.nlm.nih.gov/pubmed/33571165
http://dx.doi.org/10.1172/jci.insight.143058
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