A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria
Pine needles are used in several East Asian countries as food or traditional medicine. It contains functional components that exhibit a wide spectrum of pharmacological effects such as antioxidant, antimicrobial, anti-diabetic, and anti-inflammatory activities. We determined and characterized the no...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172577/ https://www.ncbi.nlm.nih.gov/pubmed/34093476 http://dx.doi.org/10.3389/fmicb.2021.662462 |
_version_ | 1783702558497308672 |
---|---|
author | Lee, Junho Kang, Hee Kyoung Cheong, Hyeonsook Park, Yoonkyung |
author_facet | Lee, Junho Kang, Hee Kyoung Cheong, Hyeonsook Park, Yoonkyung |
author_sort | Lee, Junho |
collection | PubMed |
description | Pine needles are used in several East Asian countries as food or traditional medicine. It contains functional components that exhibit a wide spectrum of pharmacological effects such as antioxidant, antimicrobial, anti-diabetic, and anti-inflammatory activities. We determined and characterized the novel antimicrobial peptides (AMPs) isolated from Pinus densiflora Sieb. et Zucc. The four active pine-needle (PN) peptides showed antimicrobial activity against foodborne bacteria with minimum inhibitory concentration (MIC) values within the range of 8–128 μg/ml. PN peptides showed no detectable hemolytic activity or cytotoxicity at the antimicrobial concentrations. The N-terminal amino acid sequence of the PN5 was identified using Edman degradation and Antimicrobial Peptide Database (APD) homology analysis showed that it was not identical to any other plant peptide. This suggests that PN5 can serve as an alternative therapeutic agent to be used in the food industry. |
format | Online Article Text |
id | pubmed-8172577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81725772021-06-04 A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria Lee, Junho Kang, Hee Kyoung Cheong, Hyeonsook Park, Yoonkyung Front Microbiol Microbiology Pine needles are used in several East Asian countries as food or traditional medicine. It contains functional components that exhibit a wide spectrum of pharmacological effects such as antioxidant, antimicrobial, anti-diabetic, and anti-inflammatory activities. We determined and characterized the novel antimicrobial peptides (AMPs) isolated from Pinus densiflora Sieb. et Zucc. The four active pine-needle (PN) peptides showed antimicrobial activity against foodborne bacteria with minimum inhibitory concentration (MIC) values within the range of 8–128 μg/ml. PN peptides showed no detectable hemolytic activity or cytotoxicity at the antimicrobial concentrations. The N-terminal amino acid sequence of the PN5 was identified using Edman degradation and Antimicrobial Peptide Database (APD) homology analysis showed that it was not identical to any other plant peptide. This suggests that PN5 can serve as an alternative therapeutic agent to be used in the food industry. Frontiers Media S.A. 2021-05-20 /pmc/articles/PMC8172577/ /pubmed/34093476 http://dx.doi.org/10.3389/fmicb.2021.662462 Text en Copyright © 2021 Lee, Kang, Cheong and Park. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lee, Junho Kang, Hee Kyoung Cheong, Hyeonsook Park, Yoonkyung A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria |
title | A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria |
title_full | A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria |
title_fullStr | A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria |
title_full_unstemmed | A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria |
title_short | A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria |
title_sort | novel antimicrobial peptides from pine needles of pinus densiflora sieb. et zucc. against foodborne bacteria |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172577/ https://www.ncbi.nlm.nih.gov/pubmed/34093476 http://dx.doi.org/10.3389/fmicb.2021.662462 |
work_keys_str_mv | AT leejunho anovelantimicrobialpeptidesfrompineneedlesofpinusdensiflorasiebetzuccagainstfoodbornebacteria AT kangheekyoung anovelantimicrobialpeptidesfrompineneedlesofpinusdensiflorasiebetzuccagainstfoodbornebacteria AT cheonghyeonsook anovelantimicrobialpeptidesfrompineneedlesofpinusdensiflorasiebetzuccagainstfoodbornebacteria AT parkyoonkyung anovelantimicrobialpeptidesfrompineneedlesofpinusdensiflorasiebetzuccagainstfoodbornebacteria AT leejunho novelantimicrobialpeptidesfrompineneedlesofpinusdensiflorasiebetzuccagainstfoodbornebacteria AT kangheekyoung novelantimicrobialpeptidesfrompineneedlesofpinusdensiflorasiebetzuccagainstfoodbornebacteria AT cheonghyeonsook novelantimicrobialpeptidesfrompineneedlesofpinusdensiflorasiebetzuccagainstfoodbornebacteria AT parkyoonkyung novelantimicrobialpeptidesfrompineneedlesofpinusdensiflorasiebetzuccagainstfoodbornebacteria |