The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters

The anti-inflammatory effects of the plant protease inhibitor BbCI (Bauhinia bauhinioides cruzipain inhibitor), which blocks elastase, cathepsin G, and L, and proteinase 3 has been demonstrated. Here, we investigated the recombinant rBbCI-His((6)) (containing a histidine tail) in an experimental ven...

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Autores principales: Oliveira, Cleide, Valois, Mayara Vioto, Ottaiano, Tatiana Fontes, Miranda, Antonio, Hansen, Daiane, Sampaio, Misako Uemura, Oliva, Maria Luiza Vilela, de Abreu Maffei, Francisco Humberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241853/
https://www.ncbi.nlm.nih.gov/pubmed/34188079
http://dx.doi.org/10.1038/s41598-021-92745-4
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author Oliveira, Cleide
Valois, Mayara Vioto
Ottaiano, Tatiana Fontes
Miranda, Antonio
Hansen, Daiane
Sampaio, Misako Uemura
Oliva, Maria Luiza Vilela
de Abreu Maffei, Francisco Humberto
author_facet Oliveira, Cleide
Valois, Mayara Vioto
Ottaiano, Tatiana Fontes
Miranda, Antonio
Hansen, Daiane
Sampaio, Misako Uemura
Oliva, Maria Luiza Vilela
de Abreu Maffei, Francisco Humberto
author_sort Oliveira, Cleide
collection PubMed
description The anti-inflammatory effects of the plant protease inhibitor BbCI (Bauhinia bauhinioides cruzipain inhibitor), which blocks elastase, cathepsin G, and L, and proteinase 3 has been demonstrated. Here, we investigated the recombinant rBbCI-His((6)) (containing a histidine tail) in an experimental venous thrombosis model of vena cava (VC) ligature in rats, comparing to heparin. We evaluate the effects of the inhibitors (native or recombinant) or heparin on the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in human and rat plasmas. The rats undergoing treatment received a saline solution or increasing concentrations of rBbCI-His((6)), heparin, or a mixture of both. After 4 h of ligature VC, thrombus, if present was removed and weighed. aPTT, PT, and cytokines were measured in blood collected by cardiac puncture. aPTT, PT, and bleeding time (BT) were also measured at the time of VC (vena cava) ligature. rBbCI-His((6)) (0.45 or 1.40 mg/kg) does not alter aPTT, PT or BT. No differences in coagulation parameters were detected in rBbCI-His((6)) treated rats at the time of VC ligature or when the thrombus was removed. There was a significant decrease in the weight of thrombus in the animals of the groups treated with the rBbCI-His((6)) (1.40 mg/kg), with the rBbCI-His((6)) mixture (1.40 mg/kg) + heparin (50 IU/kg) and heparin (100 IU/kg) in relation to control group (saline). The growth-related oncogene/keratinocyte chemoattractant (GRO/KC) serum levels in rats treated with rBbCI-His((6)) (1.40 mg/kg) or heparin (200 IU/kg) were reduced. In the experimental model used, rBbCI-His((6)) alone had an antithrombotic effect, not altering blood clotting or bleeding time.
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spelling pubmed-82418532021-07-06 The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters Oliveira, Cleide Valois, Mayara Vioto Ottaiano, Tatiana Fontes Miranda, Antonio Hansen, Daiane Sampaio, Misako Uemura Oliva, Maria Luiza Vilela de Abreu Maffei, Francisco Humberto Sci Rep Article The anti-inflammatory effects of the plant protease inhibitor BbCI (Bauhinia bauhinioides cruzipain inhibitor), which blocks elastase, cathepsin G, and L, and proteinase 3 has been demonstrated. Here, we investigated the recombinant rBbCI-His((6)) (containing a histidine tail) in an experimental venous thrombosis model of vena cava (VC) ligature in rats, comparing to heparin. We evaluate the effects of the inhibitors (native or recombinant) or heparin on the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in human and rat plasmas. The rats undergoing treatment received a saline solution or increasing concentrations of rBbCI-His((6)), heparin, or a mixture of both. After 4 h of ligature VC, thrombus, if present was removed and weighed. aPTT, PT, and cytokines were measured in blood collected by cardiac puncture. aPTT, PT, and bleeding time (BT) were also measured at the time of VC (vena cava) ligature. rBbCI-His((6)) (0.45 or 1.40 mg/kg) does not alter aPTT, PT or BT. No differences in coagulation parameters were detected in rBbCI-His((6)) treated rats at the time of VC ligature or when the thrombus was removed. There was a significant decrease in the weight of thrombus in the animals of the groups treated with the rBbCI-His((6)) (1.40 mg/kg), with the rBbCI-His((6)) mixture (1.40 mg/kg) + heparin (50 IU/kg) and heparin (100 IU/kg) in relation to control group (saline). The growth-related oncogene/keratinocyte chemoattractant (GRO/KC) serum levels in rats treated with rBbCI-His((6)) (1.40 mg/kg) or heparin (200 IU/kg) were reduced. In the experimental model used, rBbCI-His((6)) alone had an antithrombotic effect, not altering blood clotting or bleeding time. Nature Publishing Group UK 2021-06-29 /pmc/articles/PMC8241853/ /pubmed/34188079 http://dx.doi.org/10.1038/s41598-021-92745-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Oliveira, Cleide
Valois, Mayara Vioto
Ottaiano, Tatiana Fontes
Miranda, Antonio
Hansen, Daiane
Sampaio, Misako Uemura
Oliva, Maria Luiza Vilela
de Abreu Maffei, Francisco Humberto
The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
title The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
title_full The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
title_fullStr The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
title_full_unstemmed The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
title_short The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
title_sort recombinant plant bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241853/
https://www.ncbi.nlm.nih.gov/pubmed/34188079
http://dx.doi.org/10.1038/s41598-021-92745-4
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