Cargando…

Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells

During mitosis, the spindle undergoes morphological and dynamic changes. It reorganizes at the onset of the anaphase when the antiparallel bundler PRC1 accumulates and recruits central spindle proteins to the midzone. Little is known about how the dynamic properties of the central spindle change dur...

Descripción completa

Detalles Bibliográficos
Autores principales: Asthana, Jayant, Cade, Nicholas I, Normanno, Davide, Lim, Wei Ming, Surrey, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500333/
https://www.ncbi.nlm.nih.gov/pubmed/34580180
http://dx.doi.org/10.26508/lsa.202101222
_version_ 1784580424096284672
author Asthana, Jayant
Cade, Nicholas I
Normanno, Davide
Lim, Wei Ming
Surrey, Thomas
author_facet Asthana, Jayant
Cade, Nicholas I
Normanno, Davide
Lim, Wei Ming
Surrey, Thomas
author_sort Asthana, Jayant
collection PubMed
description During mitosis, the spindle undergoes morphological and dynamic changes. It reorganizes at the onset of the anaphase when the antiparallel bundler PRC1 accumulates and recruits central spindle proteins to the midzone. Little is known about how the dynamic properties of the central spindle change during its morphological changes in human cells. Using gene editing, we generated human cells that express from their endogenous locus fluorescent PRC1 and EB1 to quantify their native spindle distribution and binding/unbinding turnover. EB1 plus end tracking revealed a general slowdown of microtubule growth, whereas PRC1, similar to its yeast orthologue Ase1, binds increasingly strongly to compacting antiparallel microtubule overlaps. KIF4A and CLASP1 bind more dynamically to the central spindle, but also show slowing down turnover. These results show that the central spindle gradually becomes more stable during mitosis, in agreement with a recent “bundling, sliding, and compaction” model of antiparallel midzone bundle formation in the central spindle during late mitosis.
format Online
Article
Text
id pubmed-8500333
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Life Science Alliance LLC
record_format MEDLINE/PubMed
spelling pubmed-85003332021-10-26 Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells Asthana, Jayant Cade, Nicholas I Normanno, Davide Lim, Wei Ming Surrey, Thomas Life Sci Alliance Research Articles During mitosis, the spindle undergoes morphological and dynamic changes. It reorganizes at the onset of the anaphase when the antiparallel bundler PRC1 accumulates and recruits central spindle proteins to the midzone. Little is known about how the dynamic properties of the central spindle change during its morphological changes in human cells. Using gene editing, we generated human cells that express from their endogenous locus fluorescent PRC1 and EB1 to quantify their native spindle distribution and binding/unbinding turnover. EB1 plus end tracking revealed a general slowdown of microtubule growth, whereas PRC1, similar to its yeast orthologue Ase1, binds increasingly strongly to compacting antiparallel microtubule overlaps. KIF4A and CLASP1 bind more dynamically to the central spindle, but also show slowing down turnover. These results show that the central spindle gradually becomes more stable during mitosis, in agreement with a recent “bundling, sliding, and compaction” model of antiparallel midzone bundle formation in the central spindle during late mitosis. Life Science Alliance LLC 2021-09-27 /pmc/articles/PMC8500333/ /pubmed/34580180 http://dx.doi.org/10.26508/lsa.202101222 Text en © 2021 Asthana et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Asthana, Jayant
Cade, Nicholas I
Normanno, Davide
Lim, Wei Ming
Surrey, Thomas
Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells
title Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells
title_full Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells
title_fullStr Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells
title_full_unstemmed Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells
title_short Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells
title_sort gradual compaction of the central spindle decreases its dynamicity in prc1 and eb1 gene-edited cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500333/
https://www.ncbi.nlm.nih.gov/pubmed/34580180
http://dx.doi.org/10.26508/lsa.202101222
work_keys_str_mv AT asthanajayant gradualcompactionofthecentralspindledecreasesitsdynamicityinprc1andeb1geneeditedcells
AT cadenicholasi gradualcompactionofthecentralspindledecreasesitsdynamicityinprc1andeb1geneeditedcells
AT normannodavide gradualcompactionofthecentralspindledecreasesitsdynamicityinprc1andeb1geneeditedcells
AT limweiming gradualcompactionofthecentralspindledecreasesitsdynamicityinprc1andeb1geneeditedcells
AT surreythomas gradualcompactionofthecentralspindledecreasesitsdynamicityinprc1andeb1geneeditedcells