Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis
Sepsis remains a major medical emergency that describes the body's systemic immune response to an infectious process and can lead to end-stage organ dysfunction and death. Clinical studies have introduced the concept of sepsis associated encephalopathy, which seems to have a plethora of cellula...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medical University Publishing House Craiova
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551892/ https://www.ncbi.nlm.nih.gov/pubmed/34765233 http://dx.doi.org/10.12865/CHSJ.47.02.03 |
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author | ROTARU-ZAVALEANU, ALEXANDRA DANIELA NEACŞU, ALEXANDRU IONUŢ COJOCARU, ALEXANDRU OSIAC, EUGEN GHEONEA, DAN IONUT |
author_facet | ROTARU-ZAVALEANU, ALEXANDRA DANIELA NEACŞU, ALEXANDRU IONUŢ COJOCARU, ALEXANDRU OSIAC, EUGEN GHEONEA, DAN IONUT |
author_sort | ROTARU-ZAVALEANU, ALEXANDRA DANIELA |
collection | PubMed |
description | Sepsis remains a major medical emergency that describes the body's systemic immune response to an infectious process and can lead to end-stage organ dysfunction and death. Clinical studies have introduced the concept of sepsis associated encephalopathy, which seems to have a plethora of cellular and molecular triggers starting from systemic inflammatory cytokines, blood-brain barrier (BBB) rupture, microscopic brain injury, altered cerebral circulation, neurotransmission, or even metabolic dysfunction. The purpose of our study is to reproduce the sepsis model previously described using the cecal ligature and puncture (CLP), and to take a closer look to the acute modifications that occur on cellular level when it comes to the brain-blood-barrier of the mice with systemic inflammation. After a rapid systemic response to peritonitis, we show a heterogeneity in astrocytic response within different cortical structures; hippocampus having the longest change in the number of GFAP+cells, while no difference was seen in the number of cortical astrocytes. With even more increasing roles of astrocytes in different pathologies, the relation between sepsis and astrocytes could prove a valuable in discovering new therapy in sepsis. |
format | Online Article Text |
id | pubmed-8551892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Medical University Publishing House Craiova |
record_format | MEDLINE/PubMed |
spelling | pubmed-85518922021-11-10 Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis ROTARU-ZAVALEANU, ALEXANDRA DANIELA NEACŞU, ALEXANDRU IONUŢ COJOCARU, ALEXANDRU OSIAC, EUGEN GHEONEA, DAN IONUT Curr Health Sci J Original Paper Sepsis remains a major medical emergency that describes the body's systemic immune response to an infectious process and can lead to end-stage organ dysfunction and death. Clinical studies have introduced the concept of sepsis associated encephalopathy, which seems to have a plethora of cellular and molecular triggers starting from systemic inflammatory cytokines, blood-brain barrier (BBB) rupture, microscopic brain injury, altered cerebral circulation, neurotransmission, or even metabolic dysfunction. The purpose of our study is to reproduce the sepsis model previously described using the cecal ligature and puncture (CLP), and to take a closer look to the acute modifications that occur on cellular level when it comes to the brain-blood-barrier of the mice with systemic inflammation. After a rapid systemic response to peritonitis, we show a heterogeneity in astrocytic response within different cortical structures; hippocampus having the longest change in the number of GFAP+cells, while no difference was seen in the number of cortical astrocytes. With even more increasing roles of astrocytes in different pathologies, the relation between sepsis and astrocytes could prove a valuable in discovering new therapy in sepsis. Medical University Publishing House Craiova 2021 2021-06-30 /pmc/articles/PMC8551892/ /pubmed/34765233 http://dx.doi.org/10.12865/CHSJ.47.02.03 Text en Copyright © 2014, Medical University Publishing House Craiova https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License, which permits unrestricted use, adaptation, distribution and reproduction in any medium, non-commercially, provided the new creations are licensed under identical terms as the original work and the original work is properly cited. |
spellingShingle | Original Paper ROTARU-ZAVALEANU, ALEXANDRA DANIELA NEACŞU, ALEXANDRU IONUŢ COJOCARU, ALEXANDRU OSIAC, EUGEN GHEONEA, DAN IONUT Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis |
title | Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis |
title_full | Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis |
title_fullStr | Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis |
title_full_unstemmed | Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis |
title_short | Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis |
title_sort | heterogeneity in the number of astrocytes in the central nervous system after peritonitis |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551892/ https://www.ncbi.nlm.nih.gov/pubmed/34765233 http://dx.doi.org/10.12865/CHSJ.47.02.03 |
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