Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis

Sepsis remains a major medical emergency that describes the body's systemic immune response to an infectious process and can lead to end-stage organ dysfunction and death. Clinical studies have introduced the concept of sepsis associated encephalopathy, which seems to have a plethora of cellula...

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Autores principales: ROTARU-ZAVALEANU, ALEXANDRA DANIELA, NEACŞU, ALEXANDRU IONUŢ, COJOCARU, ALEXANDRU, OSIAC, EUGEN, GHEONEA, DAN IONUT
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medical University Publishing House Craiova 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551892/
https://www.ncbi.nlm.nih.gov/pubmed/34765233
http://dx.doi.org/10.12865/CHSJ.47.02.03
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author ROTARU-ZAVALEANU, ALEXANDRA DANIELA
NEACŞU, ALEXANDRU IONUŢ
COJOCARU, ALEXANDRU
OSIAC, EUGEN
GHEONEA, DAN IONUT
author_facet ROTARU-ZAVALEANU, ALEXANDRA DANIELA
NEACŞU, ALEXANDRU IONUŢ
COJOCARU, ALEXANDRU
OSIAC, EUGEN
GHEONEA, DAN IONUT
author_sort ROTARU-ZAVALEANU, ALEXANDRA DANIELA
collection PubMed
description Sepsis remains a major medical emergency that describes the body's systemic immune response to an infectious process and can lead to end-stage organ dysfunction and death. Clinical studies have introduced the concept of sepsis associated encephalopathy, which seems to have a plethora of cellular and molecular triggers starting from systemic inflammatory cytokines, blood-brain barrier (BBB) rupture, microscopic brain injury, altered cerebral circulation, neurotransmission, or even metabolic dysfunction. The purpose of our study is to reproduce the sepsis model previously described using the cecal ligature and puncture (CLP), and to take a closer look to the acute modifications that occur on cellular level when it comes to the brain-blood-barrier of the mice with systemic inflammation. After a rapid systemic response to peritonitis, we show a heterogeneity in astrocytic response within different cortical structures; hippocampus having the longest change in the number of GFAP+cells, while no difference was seen in the number of cortical astrocytes. With even more increasing roles of astrocytes in different pathologies, the relation between sepsis and astrocytes could prove a valuable in discovering new therapy in sepsis.
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spelling pubmed-85518922021-11-10 Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis ROTARU-ZAVALEANU, ALEXANDRA DANIELA NEACŞU, ALEXANDRU IONUŢ COJOCARU, ALEXANDRU OSIAC, EUGEN GHEONEA, DAN IONUT Curr Health Sci J Original Paper Sepsis remains a major medical emergency that describes the body's systemic immune response to an infectious process and can lead to end-stage organ dysfunction and death. Clinical studies have introduced the concept of sepsis associated encephalopathy, which seems to have a plethora of cellular and molecular triggers starting from systemic inflammatory cytokines, blood-brain barrier (BBB) rupture, microscopic brain injury, altered cerebral circulation, neurotransmission, or even metabolic dysfunction. The purpose of our study is to reproduce the sepsis model previously described using the cecal ligature and puncture (CLP), and to take a closer look to the acute modifications that occur on cellular level when it comes to the brain-blood-barrier of the mice with systemic inflammation. After a rapid systemic response to peritonitis, we show a heterogeneity in astrocytic response within different cortical structures; hippocampus having the longest change in the number of GFAP+cells, while no difference was seen in the number of cortical astrocytes. With even more increasing roles of astrocytes in different pathologies, the relation between sepsis and astrocytes could prove a valuable in discovering new therapy in sepsis. Medical University Publishing House Craiova 2021 2021-06-30 /pmc/articles/PMC8551892/ /pubmed/34765233 http://dx.doi.org/10.12865/CHSJ.47.02.03 Text en Copyright © 2014, Medical University Publishing House Craiova https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License, which permits unrestricted use, adaptation, distribution and reproduction in any medium, non-commercially, provided the new creations are licensed under identical terms as the original work and the original work is properly cited.
spellingShingle Original Paper
ROTARU-ZAVALEANU, ALEXANDRA DANIELA
NEACŞU, ALEXANDRU IONUŢ
COJOCARU, ALEXANDRU
OSIAC, EUGEN
GHEONEA, DAN IONUT
Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis
title Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis
title_full Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis
title_fullStr Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis
title_full_unstemmed Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis
title_short Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis
title_sort heterogeneity in the number of astrocytes in the central nervous system after peritonitis
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551892/
https://www.ncbi.nlm.nih.gov/pubmed/34765233
http://dx.doi.org/10.12865/CHSJ.47.02.03
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