LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3

BACKGROUND: LncRNA POU3F3 (POU3F3) is overexpressed and plays oncogenic roles in esophageal squamous-cell carcinomas. LncRNA MEG3 (MEG3) has been characterized as a tumor suppressive lncRNA in different types of cancer. Our preliminary deep sequencing analysis revealed the inverse correlation betwee...

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Autores principales: Liu, Yingnan, Zhuang, Yongqing, Fu, Xiaokuan, Li, Chaofei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777492/
https://www.ncbi.nlm.nih.gov/pubmed/35201451
http://dx.doi.org/10.1007/s12672-021-00414-9
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author Liu, Yingnan
Zhuang, Yongqing
Fu, Xiaokuan
Li, Chaofei
author_facet Liu, Yingnan
Zhuang, Yongqing
Fu, Xiaokuan
Li, Chaofei
author_sort Liu, Yingnan
collection PubMed
description BACKGROUND: LncRNA POU3F3 (POU3F3) is overexpressed and plays oncogenic roles in esophageal squamous-cell carcinomas. LncRNA MEG3 (MEG3) has been characterized as a tumor suppressive lncRNA in different types of cancer. Our preliminary deep sequencing analysis revealed the inverse correlation between POU3F3 and MEG2 across melanoma tissues, indicating the interaction between them in melanoma. Therefore, this study was performed to investigate the crosstalk between POU3F3 and MEG3 in melanoma. METHODS: Tumor and adjacent healthy tissues collected from 60 melanoma patients were subjected to RNA extractions and RT-qPCRs to analyze the differential expression of POU3F3 and MEG2 in melanoma. In melanoma cells, POU3F3 and MEG2 were overexpressed to study the interactions between them. CCK-8 assays were performed to analyze the roles of POU3F3 and MEG2 in regulating melanoma cell proliferation. RESULTS: We found that POU3F3 was upregulated, while lncRNA MEG3 was downregulated in melanoma. Expression levels of POU3F3 and MEG3 were inversely correlated across tumor tissues. In vitro experiments showed that POU3F3 overexpression decreased MEG3 expression in melanoma cells, while MEG3 overexpression failed to affect POU3F3. POU3F3 overexpression increased melanoma cell proliferation, while MEG3 overexpression decreased melanoma cell proliferation. In addition, rescue experiments showed that MEG3 overexpression attenuated the enhancing effects of POU3F3 overexpression. CONCLUSION: POU3F3 may promote melanoma cell proliferation by downregulating MEG3.
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spelling pubmed-87774922022-02-03 LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3 Liu, Yingnan Zhuang, Yongqing Fu, Xiaokuan Li, Chaofei Discov Oncol Research BACKGROUND: LncRNA POU3F3 (POU3F3) is overexpressed and plays oncogenic roles in esophageal squamous-cell carcinomas. LncRNA MEG3 (MEG3) has been characterized as a tumor suppressive lncRNA in different types of cancer. Our preliminary deep sequencing analysis revealed the inverse correlation between POU3F3 and MEG2 across melanoma tissues, indicating the interaction between them in melanoma. Therefore, this study was performed to investigate the crosstalk between POU3F3 and MEG3 in melanoma. METHODS: Tumor and adjacent healthy tissues collected from 60 melanoma patients were subjected to RNA extractions and RT-qPCRs to analyze the differential expression of POU3F3 and MEG2 in melanoma. In melanoma cells, POU3F3 and MEG2 were overexpressed to study the interactions between them. CCK-8 assays were performed to analyze the roles of POU3F3 and MEG2 in regulating melanoma cell proliferation. RESULTS: We found that POU3F3 was upregulated, while lncRNA MEG3 was downregulated in melanoma. Expression levels of POU3F3 and MEG3 were inversely correlated across tumor tissues. In vitro experiments showed that POU3F3 overexpression decreased MEG3 expression in melanoma cells, while MEG3 overexpression failed to affect POU3F3. POU3F3 overexpression increased melanoma cell proliferation, while MEG3 overexpression decreased melanoma cell proliferation. In addition, rescue experiments showed that MEG3 overexpression attenuated the enhancing effects of POU3F3 overexpression. CONCLUSION: POU3F3 may promote melanoma cell proliferation by downregulating MEG3. Springer US 2021-07-09 /pmc/articles/PMC8777492/ /pubmed/35201451 http://dx.doi.org/10.1007/s12672-021-00414-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Liu, Yingnan
Zhuang, Yongqing
Fu, Xiaokuan
Li, Chaofei
LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3
title LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3
title_full LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3
title_fullStr LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3
title_full_unstemmed LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3
title_short LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3
title_sort lncrna pou3f3 promotes melanoma cell proliferation by downregulating lncrna meg3
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777492/
https://www.ncbi.nlm.nih.gov/pubmed/35201451
http://dx.doi.org/10.1007/s12672-021-00414-9
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