Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics

The genus of Curtobacterium, belonging to the Microbacteriaceae family of the Actinomycetales order, includes economically significant pathogenic bacteria of soybeans and other agricultural crops. Thorough phylogenetic and full-genome analysis using the latest genomic data has demonstrated a complex...

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Autores principales: Evseev, Peter, Lukianova, Anna, Tarakanov, Rashit, Tokmakova, Anna, Shneider, Mikhail, Ignatov, Alexander, Miroshnikov, Konstantin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929003/
https://www.ncbi.nlm.nih.gov/pubmed/35723345
http://dx.doi.org/10.3390/cimb44020060
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author Evseev, Peter
Lukianova, Anna
Tarakanov, Rashit
Tokmakova, Anna
Shneider, Mikhail
Ignatov, Alexander
Miroshnikov, Konstantin
author_facet Evseev, Peter
Lukianova, Anna
Tarakanov, Rashit
Tokmakova, Anna
Shneider, Mikhail
Ignatov, Alexander
Miroshnikov, Konstantin
author_sort Evseev, Peter
collection PubMed
description The genus of Curtobacterium, belonging to the Microbacteriaceae family of the Actinomycetales order, includes economically significant pathogenic bacteria of soybeans and other agricultural crops. Thorough phylogenetic and full-genome analysis using the latest genomic data has demonstrated a complex and contradictory taxonomic picture within the group of organisms classified as the Curtobacterium species. Based on these data, it is possible to delineate about 50 new species and to reclassify a substantial part of the Curtobacterium strains. It is suggested that 53 strains, including most of the Curtobacterium flaccumfaciens pathovars, can compose a monophyletic group classified as C. flaccumfaciens. A genomic analysis using the most recent inventory of bacterial chromosomal and plasmid genomes deposited to GenBank confirmed the possible role of Microbacteriaceae plasmids in pathogenicity and demonstrated the existence of a group of related plasmids carrying virulence factors and possessing a gene distantly related to DNA polymerase found in bacteriophages and archaeal and eukaryotic viruses. A PCR diagnostic assay specific to the genus Curtobacterium was developed and tested. The presented results assist in the understanding of the evolutionary relations within the genus and can lay the foundation for further taxonomic updates.
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spelling pubmed-89290032022-06-04 Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics Evseev, Peter Lukianova, Anna Tarakanov, Rashit Tokmakova, Anna Shneider, Mikhail Ignatov, Alexander Miroshnikov, Konstantin Curr Issues Mol Biol Article The genus of Curtobacterium, belonging to the Microbacteriaceae family of the Actinomycetales order, includes economically significant pathogenic bacteria of soybeans and other agricultural crops. Thorough phylogenetic and full-genome analysis using the latest genomic data has demonstrated a complex and contradictory taxonomic picture within the group of organisms classified as the Curtobacterium species. Based on these data, it is possible to delineate about 50 new species and to reclassify a substantial part of the Curtobacterium strains. It is suggested that 53 strains, including most of the Curtobacterium flaccumfaciens pathovars, can compose a monophyletic group classified as C. flaccumfaciens. A genomic analysis using the most recent inventory of bacterial chromosomal and plasmid genomes deposited to GenBank confirmed the possible role of Microbacteriaceae plasmids in pathogenicity and demonstrated the existence of a group of related plasmids carrying virulence factors and possessing a gene distantly related to DNA polymerase found in bacteriophages and archaeal and eukaryotic viruses. A PCR diagnostic assay specific to the genus Curtobacterium was developed and tested. The presented results assist in the understanding of the evolutionary relations within the genus and can lay the foundation for further taxonomic updates. MDPI 2022-02-11 /pmc/articles/PMC8929003/ /pubmed/35723345 http://dx.doi.org/10.3390/cimb44020060 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Evseev, Peter
Lukianova, Anna
Tarakanov, Rashit
Tokmakova, Anna
Shneider, Mikhail
Ignatov, Alexander
Miroshnikov, Konstantin
Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics
title Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics
title_full Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics
title_fullStr Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics
title_full_unstemmed Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics
title_short Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics
title_sort curtobacterium spp. and curtobacterium flaccumfaciens: phylogeny, genomics-based taxonomy, pathogenicity, and diagnostics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929003/
https://www.ncbi.nlm.nih.gov/pubmed/35723345
http://dx.doi.org/10.3390/cimb44020060
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