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Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles
Herein, we report a new automated flow method based on zone fluidics for the simultaneous determination of homocysteine and homocysteine thiolactone using fluorimetric detection (λ(ext) = 370 nm/λ(em) = 480 nm). Homocysteine thiolactone is hydrolyzed on-line in alkaline medium (1 mol L(−1) NaOH) to...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000217/ https://www.ncbi.nlm.nih.gov/pubmed/35408439 http://dx.doi.org/10.3390/molecules27072040 |
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author | Tsiasioti, Apostolia Zacharis, Constantinos K. Tzanavaras, Paraskevas D. |
author_facet | Tsiasioti, Apostolia Zacharis, Constantinos K. Tzanavaras, Paraskevas D. |
author_sort | Tsiasioti, Apostolia |
collection | PubMed |
description | Herein, we report a new automated flow method based on zone fluidics for the simultaneous determination of homocysteine and homocysteine thiolactone using fluorimetric detection (λ(ext) = 370 nm/λ(em) = 480 nm). Homocysteine thiolactone is hydrolyzed on-line in alkaline medium (1 mol L(−1) NaOH) to yield homocysteine, followed by reaction with o-phthalaldehyde in a single step. Derivatization is rapid without the need of elevated temperatures and stopped-flow steps, while specificity is achieved through a unique reaction mechanism in the absence of nucleophilic compounds. Mixtures of the analytes can be analyzed quantitatively after specific separation with fluorosurfactant-capped gold nanoparticles that are selectively aggregated by homocysteine, leaving the thiolactone analogue in solution. As low as 100 nmol L(−1) of the analyte(s) can be quantified in aqueous solutions, while concentrations > 2 μmol L(−1) can be analyzed in artificial and real urine matrix following 20-fold dilution. The percent recoveries ranged between 87 and 119%. |
format | Online Article Text |
id | pubmed-9000217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90002172022-04-12 Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles Tsiasioti, Apostolia Zacharis, Constantinos K. Tzanavaras, Paraskevas D. Molecules Article Herein, we report a new automated flow method based on zone fluidics for the simultaneous determination of homocysteine and homocysteine thiolactone using fluorimetric detection (λ(ext) = 370 nm/λ(em) = 480 nm). Homocysteine thiolactone is hydrolyzed on-line in alkaline medium (1 mol L(−1) NaOH) to yield homocysteine, followed by reaction with o-phthalaldehyde in a single step. Derivatization is rapid without the need of elevated temperatures and stopped-flow steps, while specificity is achieved through a unique reaction mechanism in the absence of nucleophilic compounds. Mixtures of the analytes can be analyzed quantitatively after specific separation with fluorosurfactant-capped gold nanoparticles that are selectively aggregated by homocysteine, leaving the thiolactone analogue in solution. As low as 100 nmol L(−1) of the analyte(s) can be quantified in aqueous solutions, while concentrations > 2 μmol L(−1) can be analyzed in artificial and real urine matrix following 20-fold dilution. The percent recoveries ranged between 87 and 119%. MDPI 2022-03-22 /pmc/articles/PMC9000217/ /pubmed/35408439 http://dx.doi.org/10.3390/molecules27072040 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tsiasioti, Apostolia Zacharis, Constantinos K. Tzanavaras, Paraskevas D. Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles |
title | Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles |
title_full | Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles |
title_fullStr | Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles |
title_full_unstemmed | Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles |
title_short | Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles |
title_sort | single-step hydrolysis and derivatization of homocysteine thiolactone using zone fluidics: simultaneous analysis of mixtures with homocysteine following separation by fluorosurfactant-modified gold nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000217/ https://www.ncbi.nlm.nih.gov/pubmed/35408439 http://dx.doi.org/10.3390/molecules27072040 |
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