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Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device

Polymerase chain reaction (PCR) is limited by the long reaction time for point-of-care. Currently, commercial benchtop rapid PCR requires 30–40 min, and this time is limited by the absence of rapid and stable heating and cooling platforms rather than the biochemical reaction kinetics. This study dev...

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Autores principales: Lin, Yen-Heng, Liao, Xiang-Jun, Chang, Wei, Chiou, Chiuan-Chian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138433/
https://www.ncbi.nlm.nih.gov/pubmed/35624604
http://dx.doi.org/10.3390/bios12050303
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author Lin, Yen-Heng
Liao, Xiang-Jun
Chang, Wei
Chiou, Chiuan-Chian
author_facet Lin, Yen-Heng
Liao, Xiang-Jun
Chang, Wei
Chiou, Chiuan-Chian
author_sort Lin, Yen-Heng
collection PubMed
description Polymerase chain reaction (PCR) is limited by the long reaction time for point-of-care. Currently, commercial benchtop rapid PCR requires 30–40 min, and this time is limited by the absence of rapid and stable heating and cooling platforms rather than the biochemical reaction kinetics. This study develops an ultrafast PCR (<3 min) platform using flow-through microchannel chips. An actin gene amplicon with a length of 151 base-pairs in the whole genome was used to verify the ultrafast PCR microfluidic chip. The results demonstrated that the channel of 56 μm height can provide fast heat conduction and the channel length should not be short. Under certain denaturation and annealing/extension times, a short channel design will cause the sample to drive slowly in the microchannel with insufficient pressure in the channel, causing the fluid to generate bubbles in the high-temperature zone and subsequently destabilizing the flow. The chips used in the experiment can complete 40 thermal cycles within 160 s through a design with the 56 µm channel height and with each thermal circle measuring 4 cm long. The calculation shows that the DNA extension speed is ~60 base-pairs/s, which is consistent with the theoretical speed of the Klen Taq extension used, and the detection limit can reach 67 copies. The heat transfer time of the reagent on this platform is very short. The simple chip design and fabrication are suitable for the development of commercial ultrafast PCR chips.
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spelling pubmed-91384332022-05-28 Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device Lin, Yen-Heng Liao, Xiang-Jun Chang, Wei Chiou, Chiuan-Chian Biosensors (Basel) Article Polymerase chain reaction (PCR) is limited by the long reaction time for point-of-care. Currently, commercial benchtop rapid PCR requires 30–40 min, and this time is limited by the absence of rapid and stable heating and cooling platforms rather than the biochemical reaction kinetics. This study develops an ultrafast PCR (<3 min) platform using flow-through microchannel chips. An actin gene amplicon with a length of 151 base-pairs in the whole genome was used to verify the ultrafast PCR microfluidic chip. The results demonstrated that the channel of 56 μm height can provide fast heat conduction and the channel length should not be short. Under certain denaturation and annealing/extension times, a short channel design will cause the sample to drive slowly in the microchannel with insufficient pressure in the channel, causing the fluid to generate bubbles in the high-temperature zone and subsequently destabilizing the flow. The chips used in the experiment can complete 40 thermal cycles within 160 s through a design with the 56 µm channel height and with each thermal circle measuring 4 cm long. The calculation shows that the DNA extension speed is ~60 base-pairs/s, which is consistent with the theoretical speed of the Klen Taq extension used, and the detection limit can reach 67 copies. The heat transfer time of the reagent on this platform is very short. The simple chip design and fabrication are suitable for the development of commercial ultrafast PCR chips. MDPI 2022-05-06 /pmc/articles/PMC9138433/ /pubmed/35624604 http://dx.doi.org/10.3390/bios12050303 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Yen-Heng
Liao, Xiang-Jun
Chang, Wei
Chiou, Chiuan-Chian
Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device
title Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device
title_full Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device
title_fullStr Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device
title_full_unstemmed Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device
title_short Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device
title_sort ultrafast dna amplification using microchannel flow-through pcr device
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138433/
https://www.ncbi.nlm.nih.gov/pubmed/35624604
http://dx.doi.org/10.3390/bios12050303
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