Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis

During oocyte growth, various epigenetic modifications are gradually established, accompanied by accumulation of large amounts of mRNAs and proteins. However, little is known about the relationship between epigenetic modifications and meiotic progression. Here, by using Gdf9-Cre to achieve oocyte-sp...

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Autores principales: Meng, Tie-Gang, Lei, Wen-Long, Lu, Xukun, Liu, Xiao-Yu, Ma, Xue-Shan, Nie, Xiao-Qing, Zhao, Zheng-Hui, Li, Qian-Nan, Huang, Lin, Hou, Yi, Ouyang, Ying-Chun, Li, Lei, Tang, Tie-Shan, Schatten, Heide, Xie, Wei, Gao, Shao-Rong, Ou, Xiang-Hong, Wang, Zhen-Bo, Sun, Qing-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295060/
https://www.ncbi.nlm.nih.gov/pubmed/35864958
http://dx.doi.org/10.7150/ijbs.75298
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author Meng, Tie-Gang
Lei, Wen-Long
Lu, Xukun
Liu, Xiao-Yu
Ma, Xue-Shan
Nie, Xiao-Qing
Zhao, Zheng-Hui
Li, Qian-Nan
Huang, Lin
Hou, Yi
Ouyang, Ying-Chun
Li, Lei
Tang, Tie-Shan
Schatten, Heide
Xie, Wei
Gao, Shao-Rong
Ou, Xiang-Hong
Wang, Zhen-Bo
Sun, Qing-Yuan
author_facet Meng, Tie-Gang
Lei, Wen-Long
Lu, Xukun
Liu, Xiao-Yu
Ma, Xue-Shan
Nie, Xiao-Qing
Zhao, Zheng-Hui
Li, Qian-Nan
Huang, Lin
Hou, Yi
Ouyang, Ying-Chun
Li, Lei
Tang, Tie-Shan
Schatten, Heide
Xie, Wei
Gao, Shao-Rong
Ou, Xiang-Hong
Wang, Zhen-Bo
Sun, Qing-Yuan
author_sort Meng, Tie-Gang
collection PubMed
description During oocyte growth, various epigenetic modifications are gradually established, accompanied by accumulation of large amounts of mRNAs and proteins. However, little is known about the relationship between epigenetic modifications and meiotic progression. Here, by using Gdf9-Cre to achieve oocyte-specific ablation of Ehmt2 (Euchromatic-Histone-Lysine-Methyltransferase 2) from the primordial follicle stage, we found that female mutant mice were infertile. Oocyte-specific knockout of Ehmt2 caused failure of homologous chromosome separation independent of persistently activated SAC during the first meiosis. Further studies revealed that lacking maternal Ehmt2 affected the transcriptional level of Ccnb3, while microinjection of exogenous Ccnb3 mRNA could partly rescue the failure of homologous chromosome segregation. Of particular importance was that EHMT2 regulated ccnb3 transcriptions by regulating CTCF binding near ccnb3 gene body in genome in oocytes. In addition, the mRNA level of Ccnb3 significantly decreased in the follicles microinjected with Ctcf siRNA. Therefore, our findings highlight the novel function of maternal EHMT2 on the metaphase I-to-anaphase I transition in mouse oocytes: regulating the transcription of Ccnb3.
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spelling pubmed-92950602022-07-20 Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis Meng, Tie-Gang Lei, Wen-Long Lu, Xukun Liu, Xiao-Yu Ma, Xue-Shan Nie, Xiao-Qing Zhao, Zheng-Hui Li, Qian-Nan Huang, Lin Hou, Yi Ouyang, Ying-Chun Li, Lei Tang, Tie-Shan Schatten, Heide Xie, Wei Gao, Shao-Rong Ou, Xiang-Hong Wang, Zhen-Bo Sun, Qing-Yuan Int J Biol Sci Research Paper During oocyte growth, various epigenetic modifications are gradually established, accompanied by accumulation of large amounts of mRNAs and proteins. However, little is known about the relationship between epigenetic modifications and meiotic progression. Here, by using Gdf9-Cre to achieve oocyte-specific ablation of Ehmt2 (Euchromatic-Histone-Lysine-Methyltransferase 2) from the primordial follicle stage, we found that female mutant mice were infertile. Oocyte-specific knockout of Ehmt2 caused failure of homologous chromosome separation independent of persistently activated SAC during the first meiosis. Further studies revealed that lacking maternal Ehmt2 affected the transcriptional level of Ccnb3, while microinjection of exogenous Ccnb3 mRNA could partly rescue the failure of homologous chromosome segregation. Of particular importance was that EHMT2 regulated ccnb3 transcriptions by regulating CTCF binding near ccnb3 gene body in genome in oocytes. In addition, the mRNA level of Ccnb3 significantly decreased in the follicles microinjected with Ctcf siRNA. Therefore, our findings highlight the novel function of maternal EHMT2 on the metaphase I-to-anaphase I transition in mouse oocytes: regulating the transcription of Ccnb3. Ivyspring International Publisher 2022-07-11 /pmc/articles/PMC9295060/ /pubmed/35864958 http://dx.doi.org/10.7150/ijbs.75298 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Meng, Tie-Gang
Lei, Wen-Long
Lu, Xukun
Liu, Xiao-Yu
Ma, Xue-Shan
Nie, Xiao-Qing
Zhao, Zheng-Hui
Li, Qian-Nan
Huang, Lin
Hou, Yi
Ouyang, Ying-Chun
Li, Lei
Tang, Tie-Shan
Schatten, Heide
Xie, Wei
Gao, Shao-Rong
Ou, Xiang-Hong
Wang, Zhen-Bo
Sun, Qing-Yuan
Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis
title Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis
title_full Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis
title_fullStr Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis
title_full_unstemmed Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis
title_short Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis
title_sort maternal ehmt2 is essential for homologous chromosome segregation by regulating cyclin b3 transcription in oocyte meiosis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295060/
https://www.ncbi.nlm.nih.gov/pubmed/35864958
http://dx.doi.org/10.7150/ijbs.75298
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