A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales

Ceftazidime/avibactam (CZA) is an alternative antibiotic used for the treatment of infections caused by carbapenem-resistant Enterobacterales (CRE). However, the CZA-resistant CRE strains have been detected worldwide. Therefore, it is critical to screen CZA-resistant CRE strains in colonized patient...

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Autores principales: Zeng, Weiliang, Liao, Wenli, Zhao, Yajie, Wang, Lingbo, Shu, Hongyun, Jia, Huaiyu, Chen, Tao, Zhang, Ying, Zhou, Tieli, Wu, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315207/
https://www.ncbi.nlm.nih.gov/pubmed/35903471
http://dx.doi.org/10.3389/fmicb.2022.956044
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author Zeng, Weiliang
Liao, Wenli
Zhao, Yajie
Wang, Lingbo
Shu, Hongyun
Jia, Huaiyu
Chen, Tao
Zhang, Ying
Zhou, Tieli
Wu, Qing
author_facet Zeng, Weiliang
Liao, Wenli
Zhao, Yajie
Wang, Lingbo
Shu, Hongyun
Jia, Huaiyu
Chen, Tao
Zhang, Ying
Zhou, Tieli
Wu, Qing
author_sort Zeng, Weiliang
collection PubMed
description Ceftazidime/avibactam (CZA) is an alternative antibiotic used for the treatment of infections caused by carbapenem-resistant Enterobacterales (CRE). However, the CZA-resistant CRE strains have been detected worldwide. Therefore, it is critical to screen CZA-resistant CRE strains in colonized patients or a specific population so as to rapidly implement infection control measures to limit their transmission. In this study, we developed a Salmonella-Shigella (SS) CZA-selective medium and assessed its performance to screen for clinical CZA-resistant CRE isolates in both pure-strain specimens and stool samples. A total of 150 non-duplicated isolates, including 75 CZA-susceptible and 75 CZA-resistant CRE pathogens, were tested by using the broth microdilution method and the SS CZA medium, respectively. The bacterial suspensions were serially diluted in the SS CZA medium, which showed excellent screening performance in both pure CZA-resistant CRE strain and the stool samples with the lowest detection limit of 10(1)-10(2) and 10(1)-10(3) CFU/ml, respectively. Notably, none of the susceptible isolates showed growth even at the highest dilution concentration of 10(8) CFU/ml. Most importantly, the SS CZA medium demonstrated excellent performance in screening simulated clinical polymicrobial specimens. Moreover, its screening performance was unaffected by the different resistance determinants for tested isolates. Cumulatively, our data suggest that the SS CZA medium can be used as a promising selective medium to screen CZA-resistant CRE, irrespective of their resistance mechanisms.
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spelling pubmed-93152072022-07-27 A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales Zeng, Weiliang Liao, Wenli Zhao, Yajie Wang, Lingbo Shu, Hongyun Jia, Huaiyu Chen, Tao Zhang, Ying Zhou, Tieli Wu, Qing Front Microbiol Microbiology Ceftazidime/avibactam (CZA) is an alternative antibiotic used for the treatment of infections caused by carbapenem-resistant Enterobacterales (CRE). However, the CZA-resistant CRE strains have been detected worldwide. Therefore, it is critical to screen CZA-resistant CRE strains in colonized patients or a specific population so as to rapidly implement infection control measures to limit their transmission. In this study, we developed a Salmonella-Shigella (SS) CZA-selective medium and assessed its performance to screen for clinical CZA-resistant CRE isolates in both pure-strain specimens and stool samples. A total of 150 non-duplicated isolates, including 75 CZA-susceptible and 75 CZA-resistant CRE pathogens, were tested by using the broth microdilution method and the SS CZA medium, respectively. The bacterial suspensions were serially diluted in the SS CZA medium, which showed excellent screening performance in both pure CZA-resistant CRE strain and the stool samples with the lowest detection limit of 10(1)-10(2) and 10(1)-10(3) CFU/ml, respectively. Notably, none of the susceptible isolates showed growth even at the highest dilution concentration of 10(8) CFU/ml. Most importantly, the SS CZA medium demonstrated excellent performance in screening simulated clinical polymicrobial specimens. Moreover, its screening performance was unaffected by the different resistance determinants for tested isolates. Cumulatively, our data suggest that the SS CZA medium can be used as a promising selective medium to screen CZA-resistant CRE, irrespective of their resistance mechanisms. Frontiers Media S.A. 2022-07-12 /pmc/articles/PMC9315207/ /pubmed/35903471 http://dx.doi.org/10.3389/fmicb.2022.956044 Text en Copyright © 2022 Zeng, Liao, Zhao, Wang, Shu, Jia, Chen, Zhang, Zhou and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zeng, Weiliang
Liao, Wenli
Zhao, Yajie
Wang, Lingbo
Shu, Hongyun
Jia, Huaiyu
Chen, Tao
Zhang, Ying
Zhou, Tieli
Wu, Qing
A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales
title A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales
title_full A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales
title_fullStr A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales
title_full_unstemmed A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales
title_short A Selective Medium for Screening Ceftazidime/Avibactam Resistance in Carbapenem-Resistant Enterobacterales
title_sort selective medium for screening ceftazidime/avibactam resistance in carbapenem-resistant enterobacterales
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315207/
https://www.ncbi.nlm.nih.gov/pubmed/35903471
http://dx.doi.org/10.3389/fmicb.2022.956044
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