PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK
Myoblast differentiation is essential for the formation of skeletal muscle myofibers. Profilin1 (Pfn1) has been identified as an actin-associated protein, and has been shown to be critically important to cellular function. Our previous study found that PFN1 may inhibit the differentiation of bovine...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600610/ https://www.ncbi.nlm.nih.gov/pubmed/36291059 http://dx.doi.org/10.3390/cells11203188 |
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author | Zi, Jingjing Xu, Jing Luo, Jintang Yang, Xu Zhen, Zhen Li, Xin Hu, Debao Guo, Yiwen Guo, Hong Ding, Xiangbin Zhang, Linlin |
author_facet | Zi, Jingjing Xu, Jing Luo, Jintang Yang, Xu Zhen, Zhen Li, Xin Hu, Debao Guo, Yiwen Guo, Hong Ding, Xiangbin Zhang, Linlin |
author_sort | Zi, Jingjing |
collection | PubMed |
description | Myoblast differentiation is essential for the formation of skeletal muscle myofibers. Profilin1 (Pfn1) has been identified as an actin-associated protein, and has been shown to be critically important to cellular function. Our previous study found that PFN1 may inhibit the differentiation of bovine skeletal muscle satellite cells, but the underlying mechanism is not known. Here, we confirmed that PFN1 negatively regulated the myogenic differentiation of bovine skeletal muscle satellite cells. Immunoprecipitation assay combined with mass spectrometry showed that Cdc42 was a binding protein of PFN1. Cdc42 could be activated by PFN1 and could inhibit the myogenic differentiation like PFN1. Mechanistically, activated Cdc42 increased the phosphorylation level of p2l-activated kinase (PAK), which further activated the phosphorylation activity of c-Jun N-terminal kinase (JNK), whereas PAK and JNK are inhibitors of myogenic differentiation. Taken together, our results reveal that PFN1 is a repressor of bovine myogenic differentiation, and provide the regulatory mechanism. |
format | Online Article Text |
id | pubmed-9600610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96006102022-10-27 PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK Zi, Jingjing Xu, Jing Luo, Jintang Yang, Xu Zhen, Zhen Li, Xin Hu, Debao Guo, Yiwen Guo, Hong Ding, Xiangbin Zhang, Linlin Cells Article Myoblast differentiation is essential for the formation of skeletal muscle myofibers. Profilin1 (Pfn1) has been identified as an actin-associated protein, and has been shown to be critically important to cellular function. Our previous study found that PFN1 may inhibit the differentiation of bovine skeletal muscle satellite cells, but the underlying mechanism is not known. Here, we confirmed that PFN1 negatively regulated the myogenic differentiation of bovine skeletal muscle satellite cells. Immunoprecipitation assay combined with mass spectrometry showed that Cdc42 was a binding protein of PFN1. Cdc42 could be activated by PFN1 and could inhibit the myogenic differentiation like PFN1. Mechanistically, activated Cdc42 increased the phosphorylation level of p2l-activated kinase (PAK), which further activated the phosphorylation activity of c-Jun N-terminal kinase (JNK), whereas PAK and JNK are inhibitors of myogenic differentiation. Taken together, our results reveal that PFN1 is a repressor of bovine myogenic differentiation, and provide the regulatory mechanism. MDPI 2022-10-11 /pmc/articles/PMC9600610/ /pubmed/36291059 http://dx.doi.org/10.3390/cells11203188 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zi, Jingjing Xu, Jing Luo, Jintang Yang, Xu Zhen, Zhen Li, Xin Hu, Debao Guo, Yiwen Guo, Hong Ding, Xiangbin Zhang, Linlin PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK |
title | PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK |
title_full | PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK |
title_fullStr | PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK |
title_full_unstemmed | PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK |
title_short | PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK |
title_sort | pfn1 inhibits myogenesis of bovine myoblast cells via cdc42-pak/jnk |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600610/ https://www.ncbi.nlm.nih.gov/pubmed/36291059 http://dx.doi.org/10.3390/cells11203188 |
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