PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK

Myoblast differentiation is essential for the formation of skeletal muscle myofibers. Profilin1 (Pfn1) has been identified as an actin-associated protein, and has been shown to be critically important to cellular function. Our previous study found that PFN1 may inhibit the differentiation of bovine...

Descripción completa

Detalles Bibliográficos
Autores principales: Zi, Jingjing, Xu, Jing, Luo, Jintang, Yang, Xu, Zhen, Zhen, Li, Xin, Hu, Debao, Guo, Yiwen, Guo, Hong, Ding, Xiangbin, Zhang, Linlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600610/
https://www.ncbi.nlm.nih.gov/pubmed/36291059
http://dx.doi.org/10.3390/cells11203188
_version_ 1784816885992259584
author Zi, Jingjing
Xu, Jing
Luo, Jintang
Yang, Xu
Zhen, Zhen
Li, Xin
Hu, Debao
Guo, Yiwen
Guo, Hong
Ding, Xiangbin
Zhang, Linlin
author_facet Zi, Jingjing
Xu, Jing
Luo, Jintang
Yang, Xu
Zhen, Zhen
Li, Xin
Hu, Debao
Guo, Yiwen
Guo, Hong
Ding, Xiangbin
Zhang, Linlin
author_sort Zi, Jingjing
collection PubMed
description Myoblast differentiation is essential for the formation of skeletal muscle myofibers. Profilin1 (Pfn1) has been identified as an actin-associated protein, and has been shown to be critically important to cellular function. Our previous study found that PFN1 may inhibit the differentiation of bovine skeletal muscle satellite cells, but the underlying mechanism is not known. Here, we confirmed that PFN1 negatively regulated the myogenic differentiation of bovine skeletal muscle satellite cells. Immunoprecipitation assay combined with mass spectrometry showed that Cdc42 was a binding protein of PFN1. Cdc42 could be activated by PFN1 and could inhibit the myogenic differentiation like PFN1. Mechanistically, activated Cdc42 increased the phosphorylation level of p2l-activated kinase (PAK), which further activated the phosphorylation activity of c-Jun N-terminal kinase (JNK), whereas PAK and JNK are inhibitors of myogenic differentiation. Taken together, our results reveal that PFN1 is a repressor of bovine myogenic differentiation, and provide the regulatory mechanism.
format Online
Article
Text
id pubmed-9600610
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96006102022-10-27 PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK Zi, Jingjing Xu, Jing Luo, Jintang Yang, Xu Zhen, Zhen Li, Xin Hu, Debao Guo, Yiwen Guo, Hong Ding, Xiangbin Zhang, Linlin Cells Article Myoblast differentiation is essential for the formation of skeletal muscle myofibers. Profilin1 (Pfn1) has been identified as an actin-associated protein, and has been shown to be critically important to cellular function. Our previous study found that PFN1 may inhibit the differentiation of bovine skeletal muscle satellite cells, but the underlying mechanism is not known. Here, we confirmed that PFN1 negatively regulated the myogenic differentiation of bovine skeletal muscle satellite cells. Immunoprecipitation assay combined with mass spectrometry showed that Cdc42 was a binding protein of PFN1. Cdc42 could be activated by PFN1 and could inhibit the myogenic differentiation like PFN1. Mechanistically, activated Cdc42 increased the phosphorylation level of p2l-activated kinase (PAK), which further activated the phosphorylation activity of c-Jun N-terminal kinase (JNK), whereas PAK and JNK are inhibitors of myogenic differentiation. Taken together, our results reveal that PFN1 is a repressor of bovine myogenic differentiation, and provide the regulatory mechanism. MDPI 2022-10-11 /pmc/articles/PMC9600610/ /pubmed/36291059 http://dx.doi.org/10.3390/cells11203188 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zi, Jingjing
Xu, Jing
Luo, Jintang
Yang, Xu
Zhen, Zhen
Li, Xin
Hu, Debao
Guo, Yiwen
Guo, Hong
Ding, Xiangbin
Zhang, Linlin
PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK
title PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK
title_full PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK
title_fullStr PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK
title_full_unstemmed PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK
title_short PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK
title_sort pfn1 inhibits myogenesis of bovine myoblast cells via cdc42-pak/jnk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600610/
https://www.ncbi.nlm.nih.gov/pubmed/36291059
http://dx.doi.org/10.3390/cells11203188
work_keys_str_mv AT zijingjing pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT xujing pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT luojintang pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT yangxu pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT zhenzhen pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT lixin pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT hudebao pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT guoyiwen pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT guohong pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT dingxiangbin pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk
AT zhanglinlin pfn1inhibitsmyogenesisofbovinemyoblastcellsviacdc42pakjnk