Pentraxin 3 Inhibits Complement-driven Macrophage Activation to Restrain Granuloma Formation in Sarcoidosis

RATIONALE: Sarcoidosis is a multisystemic inflammatory disease characterized by the formation of granulomas in response to persistent stimuli. The long pentraxin PTX3 (pentraxin 3) has emerged as a component of humoral innate immunity with essential functions in the resolution of inflammation, but i...

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Autores principales: Gonçales, Relber A., Bastos, Helder Novais, Duarte-Oliveira, Cláudio, Antunes, Daniela, Sokhatska, Oksana, Jacob, Maria, Rolo, Rui, Campos, Cláudia F., Sasaki, Sergio D., Donato, Alessia, Mapelli, Sarah N., Costa, Sandra, Moura, Conceição Souto, Delgado, Luís, Morais, António, Torrado, Egídio, van de Veerdonk, Frank L., Weichhart, Thomas, Lambris, John D., Silvestre, Ricardo, Garlanda, Cecilia, Mantovani, Alberto, Cunha, Cristina, Carvalho, Agostinho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Thoracic Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704840/
https://www.ncbi.nlm.nih.gov/pubmed/35767663
http://dx.doi.org/10.1164/rccm.202112-2771OC
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author Gonçales, Relber A.
Bastos, Helder Novais
Duarte-Oliveira, Cláudio
Antunes, Daniela
Sokhatska, Oksana
Jacob, Maria
Rolo, Rui
Campos, Cláudia F.
Sasaki, Sergio D.
Donato, Alessia
Mapelli, Sarah N.
Costa, Sandra
Moura, Conceição Souto
Delgado, Luís
Morais, António
Torrado, Egídio
van de Veerdonk, Frank L.
Weichhart, Thomas
Lambris, John D.
Silvestre, Ricardo
Garlanda, Cecilia
Mantovani, Alberto
Cunha, Cristina
Carvalho, Agostinho
author_facet Gonçales, Relber A.
Bastos, Helder Novais
Duarte-Oliveira, Cláudio
Antunes, Daniela
Sokhatska, Oksana
Jacob, Maria
Rolo, Rui
Campos, Cláudia F.
Sasaki, Sergio D.
Donato, Alessia
Mapelli, Sarah N.
Costa, Sandra
Moura, Conceição Souto
Delgado, Luís
Morais, António
Torrado, Egídio
van de Veerdonk, Frank L.
Weichhart, Thomas
Lambris, John D.
Silvestre, Ricardo
Garlanda, Cecilia
Mantovani, Alberto
Cunha, Cristina
Carvalho, Agostinho
author_sort Gonçales, Relber A.
collection PubMed
description RATIONALE: Sarcoidosis is a multisystemic inflammatory disease characterized by the formation of granulomas in response to persistent stimuli. The long pentraxin PTX3 (pentraxin 3) has emerged as a component of humoral innate immunity with essential functions in the resolution of inflammation, but its role during granuloma formation is unknown. OBJECTIVES: To evaluate PTX3 as a modulator of pathogenic signals involved in granuloma formation and inflammation in sarcoidosis. METHODS: Peripheral blood mononuclear cells obtained from patients with sarcoidosis harboring loss-of-function genetic variants and gene-deleted mice were used to assess the role of PTX3 in experimental models of granuloma formation in vitro and in vivo. The identified mechanisms of granulomatous inflammation were further evaluated in tissue and BAL samples and correlated with the disease course. MEASUREMENTS AND MAIN RESULTS: We have identified a molecular link between PTX3 deficiency and the pathogenic amplification of complement activation to promote granuloma formation. Mechanistically, PTX3 deficiency licensed the complement component C5a-mediated activation of the metabolic checkpoint kinase mTORC1 (mammalian target of rapamycin complex 1) and the reprogramming of macrophages toward increased glycolysis to foster their proliferation and aggregation. This process sustained the further recruitment of granuloma-promoting immune cells and the associated proinflammatory microenvironment and influenced the clinical course of the disease. CONCLUSIONS: Our results identify PTX3 as a pivotal molecule that regulates complement-mediated signaling cues in macrophages to restrain granulomatous inflammation and highlight the therapeutic potential of this signaling axis in targeting granuloma formation in sarcoidosis.
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spelling pubmed-97048402022-11-29 Pentraxin 3 Inhibits Complement-driven Macrophage Activation to Restrain Granuloma Formation in Sarcoidosis Gonçales, Relber A. Bastos, Helder Novais Duarte-Oliveira, Cláudio Antunes, Daniela Sokhatska, Oksana Jacob, Maria Rolo, Rui Campos, Cláudia F. Sasaki, Sergio D. Donato, Alessia Mapelli, Sarah N. Costa, Sandra Moura, Conceição Souto Delgado, Luís Morais, António Torrado, Egídio van de Veerdonk, Frank L. Weichhart, Thomas Lambris, John D. Silvestre, Ricardo Garlanda, Cecilia Mantovani, Alberto Cunha, Cristina Carvalho, Agostinho Am J Respir Crit Care Med Original Articles RATIONALE: Sarcoidosis is a multisystemic inflammatory disease characterized by the formation of granulomas in response to persistent stimuli. The long pentraxin PTX3 (pentraxin 3) has emerged as a component of humoral innate immunity with essential functions in the resolution of inflammation, but its role during granuloma formation is unknown. OBJECTIVES: To evaluate PTX3 as a modulator of pathogenic signals involved in granuloma formation and inflammation in sarcoidosis. METHODS: Peripheral blood mononuclear cells obtained from patients with sarcoidosis harboring loss-of-function genetic variants and gene-deleted mice were used to assess the role of PTX3 in experimental models of granuloma formation in vitro and in vivo. The identified mechanisms of granulomatous inflammation were further evaluated in tissue and BAL samples and correlated with the disease course. MEASUREMENTS AND MAIN RESULTS: We have identified a molecular link between PTX3 deficiency and the pathogenic amplification of complement activation to promote granuloma formation. Mechanistically, PTX3 deficiency licensed the complement component C5a-mediated activation of the metabolic checkpoint kinase mTORC1 (mammalian target of rapamycin complex 1) and the reprogramming of macrophages toward increased glycolysis to foster their proliferation and aggregation. This process sustained the further recruitment of granuloma-promoting immune cells and the associated proinflammatory microenvironment and influenced the clinical course of the disease. CONCLUSIONS: Our results identify PTX3 as a pivotal molecule that regulates complement-mediated signaling cues in macrophages to restrain granulomatous inflammation and highlight the therapeutic potential of this signaling axis in targeting granuloma formation in sarcoidosis. American Thoracic Society 2022-06-29 /pmc/articles/PMC9704840/ /pubmed/35767663 http://dx.doi.org/10.1164/rccm.202112-2771OC Text en Copyright © 2022 by the American Thoracic Society https://creativecommons.org/licenses/by-nc-nd/4.0/This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . For commercial usage and reprints, please e-mail Diane Gern (dgern@thoracic.org).
spellingShingle Original Articles
Gonçales, Relber A.
Bastos, Helder Novais
Duarte-Oliveira, Cláudio
Antunes, Daniela
Sokhatska, Oksana
Jacob, Maria
Rolo, Rui
Campos, Cláudia F.
Sasaki, Sergio D.
Donato, Alessia
Mapelli, Sarah N.
Costa, Sandra
Moura, Conceição Souto
Delgado, Luís
Morais, António
Torrado, Egídio
van de Veerdonk, Frank L.
Weichhart, Thomas
Lambris, John D.
Silvestre, Ricardo
Garlanda, Cecilia
Mantovani, Alberto
Cunha, Cristina
Carvalho, Agostinho
Pentraxin 3 Inhibits Complement-driven Macrophage Activation to Restrain Granuloma Formation in Sarcoidosis
title Pentraxin 3 Inhibits Complement-driven Macrophage Activation to Restrain Granuloma Formation in Sarcoidosis
title_full Pentraxin 3 Inhibits Complement-driven Macrophage Activation to Restrain Granuloma Formation in Sarcoidosis
title_fullStr Pentraxin 3 Inhibits Complement-driven Macrophage Activation to Restrain Granuloma Formation in Sarcoidosis
title_full_unstemmed Pentraxin 3 Inhibits Complement-driven Macrophage Activation to Restrain Granuloma Formation in Sarcoidosis
title_short Pentraxin 3 Inhibits Complement-driven Macrophage Activation to Restrain Granuloma Formation in Sarcoidosis
title_sort pentraxin 3 inhibits complement-driven macrophage activation to restrain granuloma formation in sarcoidosis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704840/
https://www.ncbi.nlm.nih.gov/pubmed/35767663
http://dx.doi.org/10.1164/rccm.202112-2771OC
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