Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi

Litchi is an important commercial fruit crop widely grown in the world. Graft incompatibility between rootstocks and scions is a major constraint for large-scale cultivation of litchi orchards, popularization of new and excellent litchi varieties, and associated industrial development. Further, the...

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Autores principales: Hou, Yanjie, Qin, Xianquan, Qiu, Hongye, Li, Dongbo, Xu, Ning, Zhang, Shuwei, Fang, Chen, Li, Hongli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849251/
https://www.ncbi.nlm.nih.gov/pubmed/36685870
http://dx.doi.org/10.3389/fgene.2022.1059333
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author Hou, Yanjie
Qin, Xianquan
Qiu, Hongye
Li, Dongbo
Xu, Ning
Zhang, Shuwei
Fang, Chen
Li, Hongli
author_facet Hou, Yanjie
Qin, Xianquan
Qiu, Hongye
Li, Dongbo
Xu, Ning
Zhang, Shuwei
Fang, Chen
Li, Hongli
author_sort Hou, Yanjie
collection PubMed
description Litchi is an important commercial fruit crop widely grown in the world. Graft incompatibility between rootstocks and scions is a major constraint for large-scale cultivation of litchi orchards, popularization of new and excellent litchi varieties, and associated industrial development. Further, the genetic mechanism of graft incompatibility is still unclear in litchi. To reduce the incompatibility problems, this study investigated metabolic and transcriptomic differences between graft compatible and incompatible rootstock-scion combinations of litchi. The result of metabolomics analysis showed that incompatible rootstock-scion interaction modified the profiles of several metabolic substances. However, various compounds of flavonoids, phenolic acids, and lignin predominantly exhibited significantly altered abundance in graft incompatible combinations. Transcriptome analysis identified that graft incompatibility induces dynamic gene differences. The majority of these differentially expressed genes were enriched in biosynthetic pathways of phenylpropanoids. The differential expressions of genes in these pathways could be linked to the differential abundance levels of flavonoids, phenolic acids, and lignin compounds. Integrated metabolomic and transcriptomic analyses revealed a strong relationship between differential genes and differential metabolites identified in this study. In addition, identified hub genes and metabolites were closely associated with graft incompatibility of litchi. This study characterized the abundance of metabolites and genes in graft incompatible combinations and further discussed the genetic mechanism of graft incompatibility in litchi. Our results provide a platform to dissect the molecular mechanisms of graft incompatibility in the litchi fruit.
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spelling pubmed-98492512023-01-20 Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi Hou, Yanjie Qin, Xianquan Qiu, Hongye Li, Dongbo Xu, Ning Zhang, Shuwei Fang, Chen Li, Hongli Front Genet Genetics Litchi is an important commercial fruit crop widely grown in the world. Graft incompatibility between rootstocks and scions is a major constraint for large-scale cultivation of litchi orchards, popularization of new and excellent litchi varieties, and associated industrial development. Further, the genetic mechanism of graft incompatibility is still unclear in litchi. To reduce the incompatibility problems, this study investigated metabolic and transcriptomic differences between graft compatible and incompatible rootstock-scion combinations of litchi. The result of metabolomics analysis showed that incompatible rootstock-scion interaction modified the profiles of several metabolic substances. However, various compounds of flavonoids, phenolic acids, and lignin predominantly exhibited significantly altered abundance in graft incompatible combinations. Transcriptome analysis identified that graft incompatibility induces dynamic gene differences. The majority of these differentially expressed genes were enriched in biosynthetic pathways of phenylpropanoids. The differential expressions of genes in these pathways could be linked to the differential abundance levels of flavonoids, phenolic acids, and lignin compounds. Integrated metabolomic and transcriptomic analyses revealed a strong relationship between differential genes and differential metabolites identified in this study. In addition, identified hub genes and metabolites were closely associated with graft incompatibility of litchi. This study characterized the abundance of metabolites and genes in graft incompatible combinations and further discussed the genetic mechanism of graft incompatibility in litchi. Our results provide a platform to dissect the molecular mechanisms of graft incompatibility in the litchi fruit. Frontiers Media S.A. 2023-01-05 /pmc/articles/PMC9849251/ /pubmed/36685870 http://dx.doi.org/10.3389/fgene.2022.1059333 Text en Copyright © 2023 Hou, Qin, Qiu, Li, Xu, Zhang, Fang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Hou, Yanjie
Qin, Xianquan
Qiu, Hongye
Li, Dongbo
Xu, Ning
Zhang, Shuwei
Fang, Chen
Li, Hongli
Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi
title Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi
title_full Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi
title_fullStr Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi
title_full_unstemmed Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi
title_short Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi
title_sort metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849251/
https://www.ncbi.nlm.nih.gov/pubmed/36685870
http://dx.doi.org/10.3389/fgene.2022.1059333
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