Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi
Litchi is an important commercial fruit crop widely grown in the world. Graft incompatibility between rootstocks and scions is a major constraint for large-scale cultivation of litchi orchards, popularization of new and excellent litchi varieties, and associated industrial development. Further, the...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849251/ https://www.ncbi.nlm.nih.gov/pubmed/36685870 http://dx.doi.org/10.3389/fgene.2022.1059333 |
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author | Hou, Yanjie Qin, Xianquan Qiu, Hongye Li, Dongbo Xu, Ning Zhang, Shuwei Fang, Chen Li, Hongli |
author_facet | Hou, Yanjie Qin, Xianquan Qiu, Hongye Li, Dongbo Xu, Ning Zhang, Shuwei Fang, Chen Li, Hongli |
author_sort | Hou, Yanjie |
collection | PubMed |
description | Litchi is an important commercial fruit crop widely grown in the world. Graft incompatibility between rootstocks and scions is a major constraint for large-scale cultivation of litchi orchards, popularization of new and excellent litchi varieties, and associated industrial development. Further, the genetic mechanism of graft incompatibility is still unclear in litchi. To reduce the incompatibility problems, this study investigated metabolic and transcriptomic differences between graft compatible and incompatible rootstock-scion combinations of litchi. The result of metabolomics analysis showed that incompatible rootstock-scion interaction modified the profiles of several metabolic substances. However, various compounds of flavonoids, phenolic acids, and lignin predominantly exhibited significantly altered abundance in graft incompatible combinations. Transcriptome analysis identified that graft incompatibility induces dynamic gene differences. The majority of these differentially expressed genes were enriched in biosynthetic pathways of phenylpropanoids. The differential expressions of genes in these pathways could be linked to the differential abundance levels of flavonoids, phenolic acids, and lignin compounds. Integrated metabolomic and transcriptomic analyses revealed a strong relationship between differential genes and differential metabolites identified in this study. In addition, identified hub genes and metabolites were closely associated with graft incompatibility of litchi. This study characterized the abundance of metabolites and genes in graft incompatible combinations and further discussed the genetic mechanism of graft incompatibility in litchi. Our results provide a platform to dissect the molecular mechanisms of graft incompatibility in the litchi fruit. |
format | Online Article Text |
id | pubmed-9849251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98492512023-01-20 Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi Hou, Yanjie Qin, Xianquan Qiu, Hongye Li, Dongbo Xu, Ning Zhang, Shuwei Fang, Chen Li, Hongli Front Genet Genetics Litchi is an important commercial fruit crop widely grown in the world. Graft incompatibility between rootstocks and scions is a major constraint for large-scale cultivation of litchi orchards, popularization of new and excellent litchi varieties, and associated industrial development. Further, the genetic mechanism of graft incompatibility is still unclear in litchi. To reduce the incompatibility problems, this study investigated metabolic and transcriptomic differences between graft compatible and incompatible rootstock-scion combinations of litchi. The result of metabolomics analysis showed that incompatible rootstock-scion interaction modified the profiles of several metabolic substances. However, various compounds of flavonoids, phenolic acids, and lignin predominantly exhibited significantly altered abundance in graft incompatible combinations. Transcriptome analysis identified that graft incompatibility induces dynamic gene differences. The majority of these differentially expressed genes were enriched in biosynthetic pathways of phenylpropanoids. The differential expressions of genes in these pathways could be linked to the differential abundance levels of flavonoids, phenolic acids, and lignin compounds. Integrated metabolomic and transcriptomic analyses revealed a strong relationship between differential genes and differential metabolites identified in this study. In addition, identified hub genes and metabolites were closely associated with graft incompatibility of litchi. This study characterized the abundance of metabolites and genes in graft incompatible combinations and further discussed the genetic mechanism of graft incompatibility in litchi. Our results provide a platform to dissect the molecular mechanisms of graft incompatibility in the litchi fruit. Frontiers Media S.A. 2023-01-05 /pmc/articles/PMC9849251/ /pubmed/36685870 http://dx.doi.org/10.3389/fgene.2022.1059333 Text en Copyright © 2023 Hou, Qin, Qiu, Li, Xu, Zhang, Fang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Hou, Yanjie Qin, Xianquan Qiu, Hongye Li, Dongbo Xu, Ning Zhang, Shuwei Fang, Chen Li, Hongli Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi |
title | Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi |
title_full | Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi |
title_fullStr | Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi |
title_full_unstemmed | Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi |
title_short | Metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi |
title_sort | metabolite profiling and transcriptome analyses provide insight into the regulatory network of graft incompatibility in litchi |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849251/ https://www.ncbi.nlm.nih.gov/pubmed/36685870 http://dx.doi.org/10.3389/fgene.2022.1059333 |
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