Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish

Due to its genetic amenability coupled with advances in genome editing, zebrafish is an excellent model to examine the function of (epi)genomic elements. Here, we repurposed the Ac/Ds maize transposition system to efficiently characterise zebrafish cis-regulated elements, also known as enhancers, in...

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Detalles Bibliográficos
Autores principales: Chong-Morrison, Vanessa, Mayes, Sarah, Simões, Filipa C., Senanayake, Upeka, Carroll, Dervla S., Riley, Paul R., Wilson, Stephen W., Sauka-Spengler, Tatjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Methods & Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320716/
https://www.ncbi.nlm.nih.gov/pubmed/37367831
http://dx.doi.org/10.1242/bio.059995
Descripción
Sumario:Due to its genetic amenability coupled with advances in genome editing, zebrafish is an excellent model to examine the function of (epi)genomic elements. Here, we repurposed the Ac/Ds maize transposition system to efficiently characterise zebrafish cis-regulated elements, also known as enhancers, in F0-microinjected embryos. We further used the system to stably express guide RNAs enabling CRISPR/dCas9-interference (CRISPRi) perturbation of enhancers without disrupting the underlying genetic sequence. In addition, we probed the phenomenon of antisense transcription at two neural crest gene loci. Our study highlights the utility of Ac/Ds transposition as a new tool for transient epigenome modulation in zebrafish.

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