The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase
Separase is an evolutionarily conserved protease that is essential for chromosome segregation and cleaves cohesin Scc1/Rad21, which joins the sister chromatids together. Although mammalian separase also functions in chromosome segregation, our understanding of this process in mammals is still incomp...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063728/ https://www.ncbi.nlm.nih.gov/pubmed/16533944 http://dx.doi.org/10.1083/jcb.200511126 |
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author | Kumada, Kazuki Yao, Ryoji Kawaguchi, Tokuichi Karasawa, Mika Hoshikawa, Yutaka Ichikawa, Koji Sugitani, Yoshinobu Imoto, Issei Inazawa, Johji Sugawara, Minoru Yanagida, Mitsuhiro Noda, Tetsuo |
author_facet | Kumada, Kazuki Yao, Ryoji Kawaguchi, Tokuichi Karasawa, Mika Hoshikawa, Yutaka Ichikawa, Koji Sugitani, Yoshinobu Imoto, Issei Inazawa, Johji Sugawara, Minoru Yanagida, Mitsuhiro Noda, Tetsuo |
author_sort | Kumada, Kazuki |
collection | PubMed |
description | Separase is an evolutionarily conserved protease that is essential for chromosome segregation and cleaves cohesin Scc1/Rad21, which joins the sister chromatids together. Although mammalian separase also functions in chromosome segregation, our understanding of this process in mammals is still incomplete. We generated separase knockout mice, reporting an essential function for mammalian separase. Separase-deficient mouse embryonic fibroblasts exhibited severely restrained increases in cell number, polyploid chromosomes, and amplified centrosomes. Chromosome spreads demonstrated that multiple chromosomes connected to a centromeric region. Live observation demonstrated that the chromosomes of separase-deficient cells condensed, but failed to segregate, although subsequent cytokinesis and chromosome decondensation proceeded normally. These results establish that mammalian separase is essential for the separation of centromeres, but not of the arm regions of chromosomes. Other cell cycle events, such as mitotic exit, DNA replication, and centrosome duplication appear to occur normally. We also demonstrated that heterozygous separase-deficient cells exhibited severely restrained increases in cell number with apparently normal mitosis in the absence of securin, which is an inhibitory partner of separase. |
format | Text |
id | pubmed-2063728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20637282007-11-29 The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase Kumada, Kazuki Yao, Ryoji Kawaguchi, Tokuichi Karasawa, Mika Hoshikawa, Yutaka Ichikawa, Koji Sugitani, Yoshinobu Imoto, Issei Inazawa, Johji Sugawara, Minoru Yanagida, Mitsuhiro Noda, Tetsuo J Cell Biol Research Articles Separase is an evolutionarily conserved protease that is essential for chromosome segregation and cleaves cohesin Scc1/Rad21, which joins the sister chromatids together. Although mammalian separase also functions in chromosome segregation, our understanding of this process in mammals is still incomplete. We generated separase knockout mice, reporting an essential function for mammalian separase. Separase-deficient mouse embryonic fibroblasts exhibited severely restrained increases in cell number, polyploid chromosomes, and amplified centrosomes. Chromosome spreads demonstrated that multiple chromosomes connected to a centromeric region. Live observation demonstrated that the chromosomes of separase-deficient cells condensed, but failed to segregate, although subsequent cytokinesis and chromosome decondensation proceeded normally. These results establish that mammalian separase is essential for the separation of centromeres, but not of the arm regions of chromosomes. Other cell cycle events, such as mitotic exit, DNA replication, and centrosome duplication appear to occur normally. We also demonstrated that heterozygous separase-deficient cells exhibited severely restrained increases in cell number with apparently normal mitosis in the absence of securin, which is an inhibitory partner of separase. The Rockefeller University Press 2006-03-13 /pmc/articles/PMC2063728/ /pubmed/16533944 http://dx.doi.org/10.1083/jcb.200511126 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Kumada, Kazuki Yao, Ryoji Kawaguchi, Tokuichi Karasawa, Mika Hoshikawa, Yutaka Ichikawa, Koji Sugitani, Yoshinobu Imoto, Issei Inazawa, Johji Sugawara, Minoru Yanagida, Mitsuhiro Noda, Tetsuo The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase |
title | The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase |
title_full | The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase |
title_fullStr | The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase |
title_full_unstemmed | The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase |
title_short | The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase |
title_sort | selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063728/ https://www.ncbi.nlm.nih.gov/pubmed/16533944 http://dx.doi.org/10.1083/jcb.200511126 |
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