Kinesin-13s form rings around microtubules
Kinesin is a superfamily of motor proteins that uses the energy of adenosine triphosphate hydrolysis to move and generate force along microtubules. A notable exception to this general description is found in the kinesin-13 family that actively depolymerizes microtubules rather than actively moving a...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064489/ https://www.ncbi.nlm.nih.gov/pubmed/17015621 http://dx.doi.org/10.1083/jcb.200605194 |
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author | Tan, Dongyan Asenjo, Ana B. Mennella, Vito Sharp, David J. Sosa, Hernando |
author_facet | Tan, Dongyan Asenjo, Ana B. Mennella, Vito Sharp, David J. Sosa, Hernando |
author_sort | Tan, Dongyan |
collection | PubMed |
description | Kinesin is a superfamily of motor proteins that uses the energy of adenosine triphosphate hydrolysis to move and generate force along microtubules. A notable exception to this general description is found in the kinesin-13 family that actively depolymerizes microtubules rather than actively moving along them. This depolymerization activity is important in mitosis during chromosome segregation. It is still not fully clear by which mechanism kinesin-13s depolymerize microtubules. To address this issue, we used electron microscopy to investigate the interaction of kinesin-13s with microtubules. Surprisingly, we found that proteins of the kinesin-13 family form rings and spirals around microtubules. This is the first report of this type of oligomeric structure for any kinesin protein. These rings may allow kinesin-13s to stay at the ends of microtubules during depolymerization. |
format | Text |
id | pubmed-2064489 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20644892007-11-29 Kinesin-13s form rings around microtubules Tan, Dongyan Asenjo, Ana B. Mennella, Vito Sharp, David J. Sosa, Hernando J Cell Biol Research Articles Kinesin is a superfamily of motor proteins that uses the energy of adenosine triphosphate hydrolysis to move and generate force along microtubules. A notable exception to this general description is found in the kinesin-13 family that actively depolymerizes microtubules rather than actively moving along them. This depolymerization activity is important in mitosis during chromosome segregation. It is still not fully clear by which mechanism kinesin-13s depolymerize microtubules. To address this issue, we used electron microscopy to investigate the interaction of kinesin-13s with microtubules. Surprisingly, we found that proteins of the kinesin-13 family form rings and spirals around microtubules. This is the first report of this type of oligomeric structure for any kinesin protein. These rings may allow kinesin-13s to stay at the ends of microtubules during depolymerization. The Rockefeller University Press 2006-10-09 /pmc/articles/PMC2064489/ /pubmed/17015621 http://dx.doi.org/10.1083/jcb.200605194 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Tan, Dongyan Asenjo, Ana B. Mennella, Vito Sharp, David J. Sosa, Hernando Kinesin-13s form rings around microtubules |
title | Kinesin-13s form rings around microtubules |
title_full | Kinesin-13s form rings around microtubules |
title_fullStr | Kinesin-13s form rings around microtubules |
title_full_unstemmed | Kinesin-13s form rings around microtubules |
title_short | Kinesin-13s form rings around microtubules |
title_sort | kinesin-13s form rings around microtubules |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064489/ https://www.ncbi.nlm.nih.gov/pubmed/17015621 http://dx.doi.org/10.1083/jcb.200605194 |
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