Laminin enhances the growth of human neural stem cells in defined culture media

BACKGROUND: Human neural stem cells (hNSC) have the potential to provide novel cell-based therapies for neurodegenerative conditions such as multiple sclerosis and Parkinson's disease. In order to realise this goal, protocols need to be developed that allow for large quantities of hNSC to be cu...

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Autores principales: Hall, Peter E, Lathia, Justin D, Caldwell, Maeve A, ffrench-Constant, Charles
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2496909/
https://www.ncbi.nlm.nih.gov/pubmed/18651950
http://dx.doi.org/10.1186/1471-2202-9-71
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author Hall, Peter E
Lathia, Justin D
Caldwell, Maeve A
ffrench-Constant, Charles
author_facet Hall, Peter E
Lathia, Justin D
Caldwell, Maeve A
ffrench-Constant, Charles
author_sort Hall, Peter E
collection PubMed
description BACKGROUND: Human neural stem cells (hNSC) have the potential to provide novel cell-based therapies for neurodegenerative conditions such as multiple sclerosis and Parkinson's disease. In order to realise this goal, protocols need to be developed that allow for large quantities of hNSC to be cultured efficiently. As such, it is important to identify factors which enhance the growth of hNSC. In vivo, stem cells reside in distinct microenvironments or niches that are responsible for the maintenance of stem cell populations. A common feature of niches is the presence of the extracellular matrix molecule, laminin. Therefore, this study investigated the effect of exogenous laminin on hNSC growth. RESULTS: To measure hNSC growth, we established culture conditions using B27-supplemented medium that enable neurospheres to grow from human neural cells plated at clonal densities. Limiting dilution assays confirmed that neurospheres were derived from single cells at these densities. Laminin was found to increase hNSC numbers as measured by this neurosphere formation. The effect of laminin was to augment the proliferation/survival of the hNSC, rather than promoting the undifferentiated state. In agreement, apoptosis was reduced in dissociated neurospheres by laminin in an integrin β1-dependent manner. CONCLUSION: The addition of laminin to the culture medium enhances the growth of hNSC, and may therefore aid their large-scale production.
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spelling pubmed-24969092008-08-06 Laminin enhances the growth of human neural stem cells in defined culture media Hall, Peter E Lathia, Justin D Caldwell, Maeve A ffrench-Constant, Charles BMC Neurosci Research Article BACKGROUND: Human neural stem cells (hNSC) have the potential to provide novel cell-based therapies for neurodegenerative conditions such as multiple sclerosis and Parkinson's disease. In order to realise this goal, protocols need to be developed that allow for large quantities of hNSC to be cultured efficiently. As such, it is important to identify factors which enhance the growth of hNSC. In vivo, stem cells reside in distinct microenvironments or niches that are responsible for the maintenance of stem cell populations. A common feature of niches is the presence of the extracellular matrix molecule, laminin. Therefore, this study investigated the effect of exogenous laminin on hNSC growth. RESULTS: To measure hNSC growth, we established culture conditions using B27-supplemented medium that enable neurospheres to grow from human neural cells plated at clonal densities. Limiting dilution assays confirmed that neurospheres were derived from single cells at these densities. Laminin was found to increase hNSC numbers as measured by this neurosphere formation. The effect of laminin was to augment the proliferation/survival of the hNSC, rather than promoting the undifferentiated state. In agreement, apoptosis was reduced in dissociated neurospheres by laminin in an integrin β1-dependent manner. CONCLUSION: The addition of laminin to the culture medium enhances the growth of hNSC, and may therefore aid their large-scale production. BioMed Central 2008-07-23 /pmc/articles/PMC2496909/ /pubmed/18651950 http://dx.doi.org/10.1186/1471-2202-9-71 Text en Copyright © 2008 Hall et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hall, Peter E
Lathia, Justin D
Caldwell, Maeve A
ffrench-Constant, Charles
Laminin enhances the growth of human neural stem cells in defined culture media
title Laminin enhances the growth of human neural stem cells in defined culture media
title_full Laminin enhances the growth of human neural stem cells in defined culture media
title_fullStr Laminin enhances the growth of human neural stem cells in defined culture media
title_full_unstemmed Laminin enhances the growth of human neural stem cells in defined culture media
title_short Laminin enhances the growth of human neural stem cells in defined culture media
title_sort laminin enhances the growth of human neural stem cells in defined culture media
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2496909/
https://www.ncbi.nlm.nih.gov/pubmed/18651950
http://dx.doi.org/10.1186/1471-2202-9-71
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AT ffrenchconstantcharles lamininenhancesthegrowthofhumanneuralstemcellsindefinedculturemedia