Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock
BACKGROUND: Prolyl isomerase Pin1 may be involved in innate immunity against microbial infection, but the mechanism how Pin1 controls the innate immunity is poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Injection of lipopolysaccharide (LPS) into the mice induces inflammatory pulmonary disorder...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033895/ https://www.ncbi.nlm.nih.gov/pubmed/21326608 http://dx.doi.org/10.1371/journal.pone.0014656 |
_version_ | 1782197626519683072 |
---|---|
author | Akiyama, Hirotada Misawa, Takuma Ono, Masao Uchida, Chiyoko Uchida, Takafumi |
author_facet | Akiyama, Hirotada Misawa, Takuma Ono, Masao Uchida, Chiyoko Uchida, Takafumi |
author_sort | Akiyama, Hirotada |
collection | PubMed |
description | BACKGROUND: Prolyl isomerase Pin1 may be involved in innate immunity against microbial infection, but the mechanism how Pin1 controls the innate immunity is poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Injection of lipopolysaccharide (LPS) into the mice induces inflammatory pulmonary disorder and sometimes the serious damages lead to death. Comparing to the wild-type (WT) mice, the Pin1(−/−) mice showed more serious damages in lung and the lower survival rate after the LPS injection. We compared the levels of typical inflammatory cytokines. Pin1(−/−) mice overreacted to the LPS injection to produce inflammatory cytokines, especially IL-6 more than WT mice. We showed that Pin1 binds phosphorylated PU.1 and they localize together in a nucleus. These results suggest that Pin1 controls the transcriptional activity of PU.1 and suppresses overreaction of macrophage that causes serious damages in lung. CONCLUSIONS/SIGNIFICANCE: Pin1 may protect the mice from serious inflammation by LPS injection by attenuating the increase of IL-6 transcription of the mouse macrophages. |
format | Text |
id | pubmed-3033895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30338952011-02-15 Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock Akiyama, Hirotada Misawa, Takuma Ono, Masao Uchida, Chiyoko Uchida, Takafumi PLoS One Research Article BACKGROUND: Prolyl isomerase Pin1 may be involved in innate immunity against microbial infection, but the mechanism how Pin1 controls the innate immunity is poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Injection of lipopolysaccharide (LPS) into the mice induces inflammatory pulmonary disorder and sometimes the serious damages lead to death. Comparing to the wild-type (WT) mice, the Pin1(−/−) mice showed more serious damages in lung and the lower survival rate after the LPS injection. We compared the levels of typical inflammatory cytokines. Pin1(−/−) mice overreacted to the LPS injection to produce inflammatory cytokines, especially IL-6 more than WT mice. We showed that Pin1 binds phosphorylated PU.1 and they localize together in a nucleus. These results suggest that Pin1 controls the transcriptional activity of PU.1 and suppresses overreaction of macrophage that causes serious damages in lung. CONCLUSIONS/SIGNIFICANCE: Pin1 may protect the mice from serious inflammation by LPS injection by attenuating the increase of IL-6 transcription of the mouse macrophages. Public Library of Science 2011-02-04 /pmc/articles/PMC3033895/ /pubmed/21326608 http://dx.doi.org/10.1371/journal.pone.0014656 Text en Akiyama et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Akiyama, Hirotada Misawa, Takuma Ono, Masao Uchida, Chiyoko Uchida, Takafumi Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock |
title | Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock |
title_full | Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock |
title_fullStr | Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock |
title_full_unstemmed | Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock |
title_short | Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock |
title_sort | prolyl isomerase pin1 protects mice from endotoxin shock |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033895/ https://www.ncbi.nlm.nih.gov/pubmed/21326608 http://dx.doi.org/10.1371/journal.pone.0014656 |
work_keys_str_mv | AT akiyamahirotada prolylisomerasepin1protectsmicefromendotoxinshock AT misawatakuma prolylisomerasepin1protectsmicefromendotoxinshock AT onomasao prolylisomerasepin1protectsmicefromendotoxinshock AT uchidachiyoko prolylisomerasepin1protectsmicefromendotoxinshock AT uchidatakafumi prolylisomerasepin1protectsmicefromendotoxinshock |