Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock

BACKGROUND: Prolyl isomerase Pin1 may be involved in innate immunity against microbial infection, but the mechanism how Pin1 controls the innate immunity is poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Injection of lipopolysaccharide (LPS) into the mice induces inflammatory pulmonary disorder...

Descripción completa

Detalles Bibliográficos
Autores principales: Akiyama, Hirotada, Misawa, Takuma, Ono, Masao, Uchida, Chiyoko, Uchida, Takafumi
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033895/
https://www.ncbi.nlm.nih.gov/pubmed/21326608
http://dx.doi.org/10.1371/journal.pone.0014656
_version_ 1782197626519683072
author Akiyama, Hirotada
Misawa, Takuma
Ono, Masao
Uchida, Chiyoko
Uchida, Takafumi
author_facet Akiyama, Hirotada
Misawa, Takuma
Ono, Masao
Uchida, Chiyoko
Uchida, Takafumi
author_sort Akiyama, Hirotada
collection PubMed
description BACKGROUND: Prolyl isomerase Pin1 may be involved in innate immunity against microbial infection, but the mechanism how Pin1 controls the innate immunity is poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Injection of lipopolysaccharide (LPS) into the mice induces inflammatory pulmonary disorder and sometimes the serious damages lead to death. Comparing to the wild-type (WT) mice, the Pin1(−/−) mice showed more serious damages in lung and the lower survival rate after the LPS injection. We compared the levels of typical inflammatory cytokines. Pin1(−/−) mice overreacted to the LPS injection to produce inflammatory cytokines, especially IL-6 more than WT mice. We showed that Pin1 binds phosphorylated PU.1 and they localize together in a nucleus. These results suggest that Pin1 controls the transcriptional activity of PU.1 and suppresses overreaction of macrophage that causes serious damages in lung. CONCLUSIONS/SIGNIFICANCE: Pin1 may protect the mice from serious inflammation by LPS injection by attenuating the increase of IL-6 transcription of the mouse macrophages.
format Text
id pubmed-3033895
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30338952011-02-15 Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock Akiyama, Hirotada Misawa, Takuma Ono, Masao Uchida, Chiyoko Uchida, Takafumi PLoS One Research Article BACKGROUND: Prolyl isomerase Pin1 may be involved in innate immunity against microbial infection, but the mechanism how Pin1 controls the innate immunity is poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Injection of lipopolysaccharide (LPS) into the mice induces inflammatory pulmonary disorder and sometimes the serious damages lead to death. Comparing to the wild-type (WT) mice, the Pin1(−/−) mice showed more serious damages in lung and the lower survival rate after the LPS injection. We compared the levels of typical inflammatory cytokines. Pin1(−/−) mice overreacted to the LPS injection to produce inflammatory cytokines, especially IL-6 more than WT mice. We showed that Pin1 binds phosphorylated PU.1 and they localize together in a nucleus. These results suggest that Pin1 controls the transcriptional activity of PU.1 and suppresses overreaction of macrophage that causes serious damages in lung. CONCLUSIONS/SIGNIFICANCE: Pin1 may protect the mice from serious inflammation by LPS injection by attenuating the increase of IL-6 transcription of the mouse macrophages. Public Library of Science 2011-02-04 /pmc/articles/PMC3033895/ /pubmed/21326608 http://dx.doi.org/10.1371/journal.pone.0014656 Text en Akiyama et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Akiyama, Hirotada
Misawa, Takuma
Ono, Masao
Uchida, Chiyoko
Uchida, Takafumi
Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock
title Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock
title_full Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock
title_fullStr Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock
title_full_unstemmed Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock
title_short Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock
title_sort prolyl isomerase pin1 protects mice from endotoxin shock
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033895/
https://www.ncbi.nlm.nih.gov/pubmed/21326608
http://dx.doi.org/10.1371/journal.pone.0014656
work_keys_str_mv AT akiyamahirotada prolylisomerasepin1protectsmicefromendotoxinshock
AT misawatakuma prolylisomerasepin1protectsmicefromendotoxinshock
AT onomasao prolylisomerasepin1protectsmicefromendotoxinshock
AT uchidachiyoko prolylisomerasepin1protectsmicefromendotoxinshock
AT uchidatakafumi prolylisomerasepin1protectsmicefromendotoxinshock