Rapamycin selectively alters serum chemistry in diabetic mice

The study was undertaken to explore the effect of rapamycin, an anti-inflammatory agent, on the metabolic profile of type 2 diabetic mice. Seven-month-old diabetic db/db mice and their lean littermate non-diabetic controls (db/m) were randomized to receive control chow or chow mixed with rapamycin (...

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Autores principales: Tabatabai-Mir, Hooman, Sataranatarajan, Kavithalakshmi, Lee, Hak Joo, Bokov, Alex F., Fernandez, Elizabeth, Diaz, Vivian, Choudhury, Goutam Ghosh, Richardson, Arlan, Kasinath, Balakuntalam S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417581/
https://www.ncbi.nlm.nih.gov/pubmed/22953036
http://dx.doi.org/10.3402/pba.v2i0.15896
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author Tabatabai-Mir, Hooman
Sataranatarajan, Kavithalakshmi
Lee, Hak Joo
Bokov, Alex F.
Fernandez, Elizabeth
Diaz, Vivian
Choudhury, Goutam Ghosh
Richardson, Arlan
Kasinath, Balakuntalam S.
author_facet Tabatabai-Mir, Hooman
Sataranatarajan, Kavithalakshmi
Lee, Hak Joo
Bokov, Alex F.
Fernandez, Elizabeth
Diaz, Vivian
Choudhury, Goutam Ghosh
Richardson, Arlan
Kasinath, Balakuntalam S.
author_sort Tabatabai-Mir, Hooman
collection PubMed
description The study was undertaken to explore the effect of rapamycin, an anti-inflammatory agent, on the metabolic profile of type 2 diabetic mice. Seven-month-old diabetic db/db mice and their lean littermate non-diabetic controls (db/m) were randomized to receive control chow or chow mixed with rapamycin (2.24 mg/kg/day) (each group n =20, males and females) for 4 months and sacrificed. Serum samples were analyzed for the measurement of glucose, creatinine, blood urea nitrogen (BUN), alkaline phosphatase (ALP), alanine aminotransferase (ALT), total cholesterol, total triglyceride, and total protein, using the automated dry chemistry analysis. Rapamycin elevated serum glucose in female diabetic mice. Serum creatinine tended to be higher in diabetic mice but was not affected by rapamycin; there was no difference in BUN levels among the groups. Serum ALP was elevated in diabetic mice and rapamycin lowered it only in female diabetic mice; serum ALT levels were increased in female diabetic mice, unaffected by rapamycin. Serum total protein was elevated in diabetic mice of both genders but was not affected by rapamycin. Diabetic mice from both genders had elevated serum cholesterol and triglycerides; rapamycin did not affect serum cholesterol but decreased serum total triglycerides in male diabetic mice. We conclude that rapamycin elicits complex metabolic responses in aging diabetic mice, worsening hyperglycemia in females but improving ALP in female diabetic and total triglycerides in male diabetic mice, respectively. The metabolic effects of rapamycin should be considered while performing studies with rapamycin in mice.
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spelling pubmed-34175812012-09-05 Rapamycin selectively alters serum chemistry in diabetic mice Tabatabai-Mir, Hooman Sataranatarajan, Kavithalakshmi Lee, Hak Joo Bokov, Alex F. Fernandez, Elizabeth Diaz, Vivian Choudhury, Goutam Ghosh Richardson, Arlan Kasinath, Balakuntalam S. Pathobiol Aging Age Relat Dis Brief Reports The study was undertaken to explore the effect of rapamycin, an anti-inflammatory agent, on the metabolic profile of type 2 diabetic mice. Seven-month-old diabetic db/db mice and their lean littermate non-diabetic controls (db/m) were randomized to receive control chow or chow mixed with rapamycin (2.24 mg/kg/day) (each group n =20, males and females) for 4 months and sacrificed. Serum samples were analyzed for the measurement of glucose, creatinine, blood urea nitrogen (BUN), alkaline phosphatase (ALP), alanine aminotransferase (ALT), total cholesterol, total triglyceride, and total protein, using the automated dry chemistry analysis. Rapamycin elevated serum glucose in female diabetic mice. Serum creatinine tended to be higher in diabetic mice but was not affected by rapamycin; there was no difference in BUN levels among the groups. Serum ALP was elevated in diabetic mice and rapamycin lowered it only in female diabetic mice; serum ALT levels were increased in female diabetic mice, unaffected by rapamycin. Serum total protein was elevated in diabetic mice of both genders but was not affected by rapamycin. Diabetic mice from both genders had elevated serum cholesterol and triglycerides; rapamycin did not affect serum cholesterol but decreased serum total triglycerides in male diabetic mice. We conclude that rapamycin elicits complex metabolic responses in aging diabetic mice, worsening hyperglycemia in females but improving ALP in female diabetic and total triglycerides in male diabetic mice, respectively. The metabolic effects of rapamycin should be considered while performing studies with rapamycin in mice. Co-Action Publishing 2012-04-23 /pmc/articles/PMC3417581/ /pubmed/22953036 http://dx.doi.org/10.3402/pba.v2i0.15896 Text en © 2012 Hooman Tabatabai-Mir et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Reports
Tabatabai-Mir, Hooman
Sataranatarajan, Kavithalakshmi
Lee, Hak Joo
Bokov, Alex F.
Fernandez, Elizabeth
Diaz, Vivian
Choudhury, Goutam Ghosh
Richardson, Arlan
Kasinath, Balakuntalam S.
Rapamycin selectively alters serum chemistry in diabetic mice
title Rapamycin selectively alters serum chemistry in diabetic mice
title_full Rapamycin selectively alters serum chemistry in diabetic mice
title_fullStr Rapamycin selectively alters serum chemistry in diabetic mice
title_full_unstemmed Rapamycin selectively alters serum chemistry in diabetic mice
title_short Rapamycin selectively alters serum chemistry in diabetic mice
title_sort rapamycin selectively alters serum chemistry in diabetic mice
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417581/
https://www.ncbi.nlm.nih.gov/pubmed/22953036
http://dx.doi.org/10.3402/pba.v2i0.15896
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