Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition
BACKGROUND: Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed gene...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057286/ https://www.ncbi.nlm.nih.gov/pubmed/24926690 http://dx.doi.org/10.1371/journal.pone.0099720 |
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author | Puig-Oliveras, Anna Ramayo-Caldas, Yuliaxis Corominas, Jordi Estellé, Jordi Pérez-Montarelo, Dafne Hudson, Nicholas J. Casellas, Joaquim Folch, Josep M. Ballester, Maria |
author_facet | Puig-Oliveras, Anna Ramayo-Caldas, Yuliaxis Corominas, Jordi Estellé, Jordi Pérez-Montarelo, Dafne Hudson, Nicholas J. Casellas, Joaquim Folch, Josep M. Ballester, Maria |
author_sort | Puig-Oliveras, Anna |
collection | PubMed |
description | BACKGROUND: Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed genes between two groups of 6 sows belonging to an Iberian × Landrace backcross with extreme phenotypes according to FA profile. RESULTS: We sequenced the muscle transcriptome acquiring 787.5 M of 75 bp paired-end reads. About 85.1% of reads were mapped to the reference genome. Of the total reads, 79.1% were located in exons, 6.0% in introns and 14.9% in intergenic regions, indicating expressed regions not annotated in the reference genome. We identified a 34.5% of the intergenic regions as interspersed repetitive regions. We predicted a total of 2,372 putative proteins. Pathway analysis with 131 differentially-expressed genes revealed that the most statistically-significant metabolic pathways were related with lipid metabolism. Moreover, 18 of the differentially-expressed genes were located in genomic regions associated with IMF composition in an independent GWAS study in the same genetic background. Thus, our results indicate that the lipid metabolism of FAs is differently modulated when the FA composition in muscle differs. For instance, a high content of PUFA may reduce FA and glucose uptake resulting in an inhibition of the lipogenesis. These results are consistent with previous studies of our group analysing the liver and the adipose tissue transcriptomes providing a view of each of the main organs involved in lipid metabolism. CONCLUSIONS: The results obtained in the muscle transcriptome analysis increase the knowledge of the gene regulation of IMF deposition, FA profile and meat quality, in terms of taste and nutritional value. Besides, our results may be important in terms of human health. |
format | Online Article Text |
id | pubmed-4057286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40572862014-06-18 Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition Puig-Oliveras, Anna Ramayo-Caldas, Yuliaxis Corominas, Jordi Estellé, Jordi Pérez-Montarelo, Dafne Hudson, Nicholas J. Casellas, Joaquim Folch, Josep M. Ballester, Maria PLoS One Research Article BACKGROUND: Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed genes between two groups of 6 sows belonging to an Iberian × Landrace backcross with extreme phenotypes according to FA profile. RESULTS: We sequenced the muscle transcriptome acquiring 787.5 M of 75 bp paired-end reads. About 85.1% of reads were mapped to the reference genome. Of the total reads, 79.1% were located in exons, 6.0% in introns and 14.9% in intergenic regions, indicating expressed regions not annotated in the reference genome. We identified a 34.5% of the intergenic regions as interspersed repetitive regions. We predicted a total of 2,372 putative proteins. Pathway analysis with 131 differentially-expressed genes revealed that the most statistically-significant metabolic pathways were related with lipid metabolism. Moreover, 18 of the differentially-expressed genes were located in genomic regions associated with IMF composition in an independent GWAS study in the same genetic background. Thus, our results indicate that the lipid metabolism of FAs is differently modulated when the FA composition in muscle differs. For instance, a high content of PUFA may reduce FA and glucose uptake resulting in an inhibition of the lipogenesis. These results are consistent with previous studies of our group analysing the liver and the adipose tissue transcriptomes providing a view of each of the main organs involved in lipid metabolism. CONCLUSIONS: The results obtained in the muscle transcriptome analysis increase the knowledge of the gene regulation of IMF deposition, FA profile and meat quality, in terms of taste and nutritional value. Besides, our results may be important in terms of human health. Public Library of Science 2014-06-13 /pmc/articles/PMC4057286/ /pubmed/24926690 http://dx.doi.org/10.1371/journal.pone.0099720 Text en © 2014 Puig-Oliveras et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Puig-Oliveras, Anna Ramayo-Caldas, Yuliaxis Corominas, Jordi Estellé, Jordi Pérez-Montarelo, Dafne Hudson, Nicholas J. Casellas, Joaquim Folch, Josep M. Ballester, Maria Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition |
title | Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition |
title_full | Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition |
title_fullStr | Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition |
title_full_unstemmed | Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition |
title_short | Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition |
title_sort | differences in muscle transcriptome among pigs phenotypically extreme for fatty acid composition |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057286/ https://www.ncbi.nlm.nih.gov/pubmed/24926690 http://dx.doi.org/10.1371/journal.pone.0099720 |
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