Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition

BACKGROUND: Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed gene...

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Autores principales: Puig-Oliveras, Anna, Ramayo-Caldas, Yuliaxis, Corominas, Jordi, Estellé, Jordi, Pérez-Montarelo, Dafne, Hudson, Nicholas J., Casellas, Joaquim, Folch, Josep M., Ballester, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057286/
https://www.ncbi.nlm.nih.gov/pubmed/24926690
http://dx.doi.org/10.1371/journal.pone.0099720
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author Puig-Oliveras, Anna
Ramayo-Caldas, Yuliaxis
Corominas, Jordi
Estellé, Jordi
Pérez-Montarelo, Dafne
Hudson, Nicholas J.
Casellas, Joaquim
Folch, Josep M.
Ballester, Maria
author_facet Puig-Oliveras, Anna
Ramayo-Caldas, Yuliaxis
Corominas, Jordi
Estellé, Jordi
Pérez-Montarelo, Dafne
Hudson, Nicholas J.
Casellas, Joaquim
Folch, Josep M.
Ballester, Maria
author_sort Puig-Oliveras, Anna
collection PubMed
description BACKGROUND: Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed genes between two groups of 6 sows belonging to an Iberian × Landrace backcross with extreme phenotypes according to FA profile. RESULTS: We sequenced the muscle transcriptome acquiring 787.5 M of 75 bp paired-end reads. About 85.1% of reads were mapped to the reference genome. Of the total reads, 79.1% were located in exons, 6.0% in introns and 14.9% in intergenic regions, indicating expressed regions not annotated in the reference genome. We identified a 34.5% of the intergenic regions as interspersed repetitive regions. We predicted a total of 2,372 putative proteins. Pathway analysis with 131 differentially-expressed genes revealed that the most statistically-significant metabolic pathways were related with lipid metabolism. Moreover, 18 of the differentially-expressed genes were located in genomic regions associated with IMF composition in an independent GWAS study in the same genetic background. Thus, our results indicate that the lipid metabolism of FAs is differently modulated when the FA composition in muscle differs. For instance, a high content of PUFA may reduce FA and glucose uptake resulting in an inhibition of the lipogenesis. These results are consistent with previous studies of our group analysing the liver and the adipose tissue transcriptomes providing a view of each of the main organs involved in lipid metabolism. CONCLUSIONS: The results obtained in the muscle transcriptome analysis increase the knowledge of the gene regulation of IMF deposition, FA profile and meat quality, in terms of taste and nutritional value. Besides, our results may be important in terms of human health.
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spelling pubmed-40572862014-06-18 Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition Puig-Oliveras, Anna Ramayo-Caldas, Yuliaxis Corominas, Jordi Estellé, Jordi Pérez-Montarelo, Dafne Hudson, Nicholas J. Casellas, Joaquim Folch, Josep M. Ballester, Maria PLoS One Research Article BACKGROUND: Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed genes between two groups of 6 sows belonging to an Iberian × Landrace backcross with extreme phenotypes according to FA profile. RESULTS: We sequenced the muscle transcriptome acquiring 787.5 M of 75 bp paired-end reads. About 85.1% of reads were mapped to the reference genome. Of the total reads, 79.1% were located in exons, 6.0% in introns and 14.9% in intergenic regions, indicating expressed regions not annotated in the reference genome. We identified a 34.5% of the intergenic regions as interspersed repetitive regions. We predicted a total of 2,372 putative proteins. Pathway analysis with 131 differentially-expressed genes revealed that the most statistically-significant metabolic pathways were related with lipid metabolism. Moreover, 18 of the differentially-expressed genes were located in genomic regions associated with IMF composition in an independent GWAS study in the same genetic background. Thus, our results indicate that the lipid metabolism of FAs is differently modulated when the FA composition in muscle differs. For instance, a high content of PUFA may reduce FA and glucose uptake resulting in an inhibition of the lipogenesis. These results are consistent with previous studies of our group analysing the liver and the adipose tissue transcriptomes providing a view of each of the main organs involved in lipid metabolism. CONCLUSIONS: The results obtained in the muscle transcriptome analysis increase the knowledge of the gene regulation of IMF deposition, FA profile and meat quality, in terms of taste and nutritional value. Besides, our results may be important in terms of human health. Public Library of Science 2014-06-13 /pmc/articles/PMC4057286/ /pubmed/24926690 http://dx.doi.org/10.1371/journal.pone.0099720 Text en © 2014 Puig-Oliveras et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Puig-Oliveras, Anna
Ramayo-Caldas, Yuliaxis
Corominas, Jordi
Estellé, Jordi
Pérez-Montarelo, Dafne
Hudson, Nicholas J.
Casellas, Joaquim
Folch, Josep M.
Ballester, Maria
Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition
title Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition
title_full Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition
title_fullStr Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition
title_full_unstemmed Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition
title_short Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition
title_sort differences in muscle transcriptome among pigs phenotypically extreme for fatty acid composition
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057286/
https://www.ncbi.nlm.nih.gov/pubmed/24926690
http://dx.doi.org/10.1371/journal.pone.0099720
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