Targeting cysteine rich C1 domain of Scaffold protein Kinase Suppressor of Ras (KSR) with anthocyanidins and flavonoids – a binding affinity characterization study

Kinase Suppressor of Ras (KSR) is a molecular scaffold that interacts with the core kinase components of the ERK cascade, Raf, MEK, ERK to provide spatial and temporal regulation of Ras-dependent ERK cascade signaling. Interruption of this mechanism can have a high influence in inhibiting the downst...

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Autores principales: Karthik, Dhananjayan, Majumder, Pulak, Palanisamy, Sivanandy, Khairunnisa, Kalathil, Venugopal, Varsha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209367/
https://www.ncbi.nlm.nih.gov/pubmed/25352726
http://dx.doi.org/10.6026/97320630010580
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author Karthik, Dhananjayan
Majumder, Pulak
Palanisamy, Sivanandy
Khairunnisa, Kalathil
Venugopal, Varsha
author_facet Karthik, Dhananjayan
Majumder, Pulak
Palanisamy, Sivanandy
Khairunnisa, Kalathil
Venugopal, Varsha
author_sort Karthik, Dhananjayan
collection PubMed
description Kinase Suppressor of Ras (KSR) is a molecular scaffold that interacts with the core kinase components of the ERK cascade, Raf, MEK, ERK to provide spatial and temporal regulation of Ras-dependent ERK cascade signaling. Interruption of this mechanism can have a high influence in inhibiting the downstream signaling of the mutated tyrosine kinase receptor kinase upon ligand binding. Still none of the studies targeted to prevent the binding of Raf, MEK binding on kinase suppressor of RAS. In that perspective the cysteine rich C1 domain of scaffold proteins kinase suppressor of Ras-1 was targeted rather than its ATP binding site with small ligand molecules like flavones and anthocyanidins and analyzed through insilico docking studies. The binding energy evaluation shows the importance of hydroxyl groups at various positions on the flavone and anthocyanidin nucleus. Over all binding interaction shows these ligands occupied the potential sites of cysteine rich C1 domain of scaffold protein KSR.
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spelling pubmed-42093672014-10-28 Targeting cysteine rich C1 domain of Scaffold protein Kinase Suppressor of Ras (KSR) with anthocyanidins and flavonoids – a binding affinity characterization study Karthik, Dhananjayan Majumder, Pulak Palanisamy, Sivanandy Khairunnisa, Kalathil Venugopal, Varsha Bioinformation Hypothesis Kinase Suppressor of Ras (KSR) is a molecular scaffold that interacts with the core kinase components of the ERK cascade, Raf, MEK, ERK to provide spatial and temporal regulation of Ras-dependent ERK cascade signaling. Interruption of this mechanism can have a high influence in inhibiting the downstream signaling of the mutated tyrosine kinase receptor kinase upon ligand binding. Still none of the studies targeted to prevent the binding of Raf, MEK binding on kinase suppressor of RAS. In that perspective the cysteine rich C1 domain of scaffold proteins kinase suppressor of Ras-1 was targeted rather than its ATP binding site with small ligand molecules like flavones and anthocyanidins and analyzed through insilico docking studies. The binding energy evaluation shows the importance of hydroxyl groups at various positions on the flavone and anthocyanidin nucleus. Over all binding interaction shows these ligands occupied the potential sites of cysteine rich C1 domain of scaffold protein KSR. Biomedical Informatics 2014-09-30 /pmc/articles/PMC4209367/ /pubmed/25352726 http://dx.doi.org/10.6026/97320630010580 Text en © 2014 Biomedical Informatics This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.
spellingShingle Hypothesis
Karthik, Dhananjayan
Majumder, Pulak
Palanisamy, Sivanandy
Khairunnisa, Kalathil
Venugopal, Varsha
Targeting cysteine rich C1 domain of Scaffold protein Kinase Suppressor of Ras (KSR) with anthocyanidins and flavonoids – a binding affinity characterization study
title Targeting cysteine rich C1 domain of Scaffold protein Kinase Suppressor of Ras (KSR) with anthocyanidins and flavonoids – a binding affinity characterization study
title_full Targeting cysteine rich C1 domain of Scaffold protein Kinase Suppressor of Ras (KSR) with anthocyanidins and flavonoids – a binding affinity characterization study
title_fullStr Targeting cysteine rich C1 domain of Scaffold protein Kinase Suppressor of Ras (KSR) with anthocyanidins and flavonoids – a binding affinity characterization study
title_full_unstemmed Targeting cysteine rich C1 domain of Scaffold protein Kinase Suppressor of Ras (KSR) with anthocyanidins and flavonoids – a binding affinity characterization study
title_short Targeting cysteine rich C1 domain of Scaffold protein Kinase Suppressor of Ras (KSR) with anthocyanidins and flavonoids – a binding affinity characterization study
title_sort targeting cysteine rich c1 domain of scaffold protein kinase suppressor of ras (ksr) with anthocyanidins and flavonoids – a binding affinity characterization study
topic Hypothesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209367/
https://www.ncbi.nlm.nih.gov/pubmed/25352726
http://dx.doi.org/10.6026/97320630010580
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