Photocatalytic Nanolithography of Self-Assembled Monolayers and Proteins

[Image: see text] Self-assembled monolayers of alkylthiolates on gold and alkylsilanes on silicon dioxide have been patterned photocatalytically on sub-100 nm length-scales using both apertured near-field and apertureless methods. Apertured lithography was carried out by means of an argon ion laser...

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Autores principales: Ul-Haq, Ehtsham, Patole, Samson, Moxey, Mark, Amstad, Esther, Vasilev, Cvetelin, Hunter, C. Neil, Leggett, Graham J., Spencer, Nicholas D., Williams, Nicholas H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2013
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327559/
https://www.ncbi.nlm.nih.gov/pubmed/23971891
http://dx.doi.org/10.1021/nn402063b
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author Ul-Haq, Ehtsham
Patole, Samson
Moxey, Mark
Amstad, Esther
Vasilev, Cvetelin
Hunter, C. Neil
Leggett, Graham J.
Spencer, Nicholas D.
Williams, Nicholas H.
author_facet Ul-Haq, Ehtsham
Patole, Samson
Moxey, Mark
Amstad, Esther
Vasilev, Cvetelin
Hunter, C. Neil
Leggett, Graham J.
Spencer, Nicholas D.
Williams, Nicholas H.
author_sort Ul-Haq, Ehtsham
collection PubMed
description [Image: see text] Self-assembled monolayers of alkylthiolates on gold and alkylsilanes on silicon dioxide have been patterned photocatalytically on sub-100 nm length-scales using both apertured near-field and apertureless methods. Apertured lithography was carried out by means of an argon ion laser (364 nm) coupled to cantilever-type near-field probes with a thin film of titania deposited over the aperture. Apertureless lithography was carried out with a helium–cadmium laser (325 nm) to excite titanium-coated, contact-mode atomic force microscope (AFM) probes. This latter approach is readily implementable on any commercial AFM system. Photodegradation occurred in both cases through the localized photocatalytic degradation of the monolayer. For alkanethiols, degradation of one thiol exposed the bare substrate, enabling refunctionalization of the bare gold by a second, contrasting thiol. For alkylsilanes, degradation of the adsorbate molecule provided a facile means for protein patterning. Lines were written in a protein-resistant film formed by the adsorption of oligo(ethylene glycol)-functionalized trichlorosilanes on glass, leading to the formation of sub-100 nm adhesive, aldehyde-functionalized regions. These were derivatized with aminobutylnitrilotriacetic acid, and complexed with Ni(2+), enabling the binding of histidine-labeled green fluorescent protein, which yielded bright fluorescence from 70-nm-wide lines that could be imaged clearly in a confocal microscope.
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spelling pubmed-43275592015-02-19 Photocatalytic Nanolithography of Self-Assembled Monolayers and Proteins Ul-Haq, Ehtsham Patole, Samson Moxey, Mark Amstad, Esther Vasilev, Cvetelin Hunter, C. Neil Leggett, Graham J. Spencer, Nicholas D. Williams, Nicholas H. ACS Nano [Image: see text] Self-assembled monolayers of alkylthiolates on gold and alkylsilanes on silicon dioxide have been patterned photocatalytically on sub-100 nm length-scales using both apertured near-field and apertureless methods. Apertured lithography was carried out by means of an argon ion laser (364 nm) coupled to cantilever-type near-field probes with a thin film of titania deposited over the aperture. Apertureless lithography was carried out with a helium–cadmium laser (325 nm) to excite titanium-coated, contact-mode atomic force microscope (AFM) probes. This latter approach is readily implementable on any commercial AFM system. Photodegradation occurred in both cases through the localized photocatalytic degradation of the monolayer. For alkanethiols, degradation of one thiol exposed the bare substrate, enabling refunctionalization of the bare gold by a second, contrasting thiol. For alkylsilanes, degradation of the adsorbate molecule provided a facile means for protein patterning. Lines were written in a protein-resistant film formed by the adsorption of oligo(ethylene glycol)-functionalized trichlorosilanes on glass, leading to the formation of sub-100 nm adhesive, aldehyde-functionalized regions. These were derivatized with aminobutylnitrilotriacetic acid, and complexed with Ni(2+), enabling the binding of histidine-labeled green fluorescent protein, which yielded bright fluorescence from 70-nm-wide lines that could be imaged clearly in a confocal microscope. American Chemical Society 2013-08-23 2013-09-24 /pmc/articles/PMC4327559/ /pubmed/23971891 http://dx.doi.org/10.1021/nn402063b Text en Copyright © 2013 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Ul-Haq, Ehtsham
Patole, Samson
Moxey, Mark
Amstad, Esther
Vasilev, Cvetelin
Hunter, C. Neil
Leggett, Graham J.
Spencer, Nicholas D.
Williams, Nicholas H.
Photocatalytic Nanolithography of Self-Assembled Monolayers and Proteins
title Photocatalytic Nanolithography of Self-Assembled Monolayers and Proteins
title_full Photocatalytic Nanolithography of Self-Assembled Monolayers and Proteins
title_fullStr Photocatalytic Nanolithography of Self-Assembled Monolayers and Proteins
title_full_unstemmed Photocatalytic Nanolithography of Self-Assembled Monolayers and Proteins
title_short Photocatalytic Nanolithography of Self-Assembled Monolayers and Proteins
title_sort photocatalytic nanolithography of self-assembled monolayers and proteins
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327559/
https://www.ncbi.nlm.nih.gov/pubmed/23971891
http://dx.doi.org/10.1021/nn402063b
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