16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform

BACKGROUND: Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer seque...

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Detalles Bibliográficos
Autores principales: Fouhy, Fiona, Clooney, Adam G., Stanton, Catherine, Claesson, Marcus J., Cotter, Paul D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4921037/
https://www.ncbi.nlm.nih.gov/pubmed/27342980
http://dx.doi.org/10.1186/s12866-016-0738-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4921037/
https://www.ncbi.nlm.nih.gov/pubmed/27342980
http://dx.doi.org/10.1186/s12866-016-0738-z

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