Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products

Hepatitis E virus (HEV), the cause of self-limiting acute hepatitis in humans, is widespread and endemic in many parts of the world. The foodborne transmission of HEV has become of concern due to the identification of undercooked pork products as a risk factor for infection. Foodborne enteric viruse...

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Autores principales: Martin-Latil, Sandra, Hennechart-Collette, Catherine, Delannoy, Sabine, Guillier, Laurent, Fach, Patrick, Perelle, Sylvie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971014/
https://www.ncbi.nlm.nih.gov/pubmed/27536278
http://dx.doi.org/10.3389/fmicb.2016.01183
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author Martin-Latil, Sandra
Hennechart-Collette, Catherine
Delannoy, Sabine
Guillier, Laurent
Fach, Patrick
Perelle, Sylvie
author_facet Martin-Latil, Sandra
Hennechart-Collette, Catherine
Delannoy, Sabine
Guillier, Laurent
Fach, Patrick
Perelle, Sylvie
author_sort Martin-Latil, Sandra
collection PubMed
description Hepatitis E virus (HEV), the cause of self-limiting acute hepatitis in humans, is widespread and endemic in many parts of the world. The foodborne transmission of HEV has become of concern due to the identification of undercooked pork products as a risk factor for infection. Foodborne enteric viruses are conventionally processed by quantitative RT-PCR (RT-qPCR), which gives sensitive and quantitative detection results. Recently, digital PCR (dPCR) has been described as a novel approach to genome quantification with no need for a standard curve. The performance of microfluidic digital RT-PCR (RT-dPCR) was compared to RT-qPCR when detecting HEV in pig liver products. The sensitivity of the RT-dPCR assay was similar to that of RT-qPCR, and quantitative data obtained by both detection methods were not significantly different for almost all samples. This absolute quantification approach may be useful for standardizing quantification of HEV in food samples and may be extended to quantifying other human pathogens in food samples.
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spelling pubmed-49710142016-08-17 Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products Martin-Latil, Sandra Hennechart-Collette, Catherine Delannoy, Sabine Guillier, Laurent Fach, Patrick Perelle, Sylvie Front Microbiol Microbiology Hepatitis E virus (HEV), the cause of self-limiting acute hepatitis in humans, is widespread and endemic in many parts of the world. The foodborne transmission of HEV has become of concern due to the identification of undercooked pork products as a risk factor for infection. Foodborne enteric viruses are conventionally processed by quantitative RT-PCR (RT-qPCR), which gives sensitive and quantitative detection results. Recently, digital PCR (dPCR) has been described as a novel approach to genome quantification with no need for a standard curve. The performance of microfluidic digital RT-PCR (RT-dPCR) was compared to RT-qPCR when detecting HEV in pig liver products. The sensitivity of the RT-dPCR assay was similar to that of RT-qPCR, and quantitative data obtained by both detection methods were not significantly different for almost all samples. This absolute quantification approach may be useful for standardizing quantification of HEV in food samples and may be extended to quantifying other human pathogens in food samples. Frontiers Media S.A. 2016-08-03 /pmc/articles/PMC4971014/ /pubmed/27536278 http://dx.doi.org/10.3389/fmicb.2016.01183 Text en Copyright © 2016 Martin-Latil, Hennechart-Collette, Delannoy, Guillier, Fach and Perelle. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Martin-Latil, Sandra
Hennechart-Collette, Catherine
Delannoy, Sabine
Guillier, Laurent
Fach, Patrick
Perelle, Sylvie
Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products
title Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products
title_full Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products
title_fullStr Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products
title_full_unstemmed Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products
title_short Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products
title_sort quantification of hepatitis e virus in naturally-contaminated pig liver products
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971014/
https://www.ncbi.nlm.nih.gov/pubmed/27536278
http://dx.doi.org/10.3389/fmicb.2016.01183
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