Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population

West Nile Virus (WNV) is a mosquito-transmitted virus from the Flaviviridae family that causes fever in 1 in 5 infected people. WNV can also become neuro-invasive and cross the blood-brain barrier leading to severe neurological symptoms in a subset of WNV infected individuals [1]. WNV neuro-invasion...

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Autores principales: Green, Richard, Wilkins, Courtney, Thomas, Sunil, Sekine, Aimee, Ireton, Renee C., Ferris, Martin T., Hendrick, Duncan M., Voss, Kathleen, Pardo-Manuel de Villena, Fernando, Baric, Ralph S., Heise, Mark T., Gale, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107684/
https://www.ncbi.nlm.nih.gov/pubmed/27872814
http://dx.doi.org/10.1016/j.gdata.2016.10.005
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author Green, Richard
Wilkins, Courtney
Thomas, Sunil
Sekine, Aimee
Ireton, Renee C.
Ferris, Martin T.
Hendrick, Duncan M.
Voss, Kathleen
Pardo-Manuel de Villena, Fernando
Baric, Ralph S.
Heise, Mark T.
Gale, Michael
author_facet Green, Richard
Wilkins, Courtney
Thomas, Sunil
Sekine, Aimee
Ireton, Renee C.
Ferris, Martin T.
Hendrick, Duncan M.
Voss, Kathleen
Pardo-Manuel de Villena, Fernando
Baric, Ralph S.
Heise, Mark T.
Gale, Michael
author_sort Green, Richard
collection PubMed
description West Nile Virus (WNV) is a mosquito-transmitted virus from the Flaviviridae family that causes fever in 1 in 5 infected people. WNV can also become neuro-invasive and cross the blood-brain barrier leading to severe neurological symptoms in a subset of WNV infected individuals [1]. WNV neuro-invasion is believed to be influenced by a number of factors including host genetics. In order to explore these effects and recapitulate the complex immune genetic differences among individuals, we studied gene expression following WNV infection in the Collaborative Cross (CC) model. The CC is a mouse genetics resource composed of > 70 independently bred, octo-parental recombinant inbred mouse lines [2]. To identify the individual host gene expression signatures influencing protection or susceptibility to WNV disease and WNV neuroinvasion, we used the nanostring nsolver platform to quantify gene expression in brain tissue isolated from WNV-infected CC mice at days 4, 7 and 12 post-infection [3]. This nanostring technology provided a high throughput, non-amplification based mRNA quantitation method to detect immune genes involved in neuro-invasion. Data was deposited into the Gene Expression Omnibus (GEO) under accession GSE85999.
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spelling pubmed-51076842016-11-21 Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population Green, Richard Wilkins, Courtney Thomas, Sunil Sekine, Aimee Ireton, Renee C. Ferris, Martin T. Hendrick, Duncan M. Voss, Kathleen Pardo-Manuel de Villena, Fernando Baric, Ralph S. Heise, Mark T. Gale, Michael Genom Data Data in Brief West Nile Virus (WNV) is a mosquito-transmitted virus from the Flaviviridae family that causes fever in 1 in 5 infected people. WNV can also become neuro-invasive and cross the blood-brain barrier leading to severe neurological symptoms in a subset of WNV infected individuals [1]. WNV neuro-invasion is believed to be influenced by a number of factors including host genetics. In order to explore these effects and recapitulate the complex immune genetic differences among individuals, we studied gene expression following WNV infection in the Collaborative Cross (CC) model. The CC is a mouse genetics resource composed of > 70 independently bred, octo-parental recombinant inbred mouse lines [2]. To identify the individual host gene expression signatures influencing protection or susceptibility to WNV disease and WNV neuroinvasion, we used the nanostring nsolver platform to quantify gene expression in brain tissue isolated from WNV-infected CC mice at days 4, 7 and 12 post-infection [3]. This nanostring technology provided a high throughput, non-amplification based mRNA quantitation method to detect immune genes involved in neuro-invasion. Data was deposited into the Gene Expression Omnibus (GEO) under accession GSE85999. Elsevier 2016-10-14 /pmc/articles/PMC5107684/ /pubmed/27872814 http://dx.doi.org/10.1016/j.gdata.2016.10.005 Text en http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data in Brief
Green, Richard
Wilkins, Courtney
Thomas, Sunil
Sekine, Aimee
Ireton, Renee C.
Ferris, Martin T.
Hendrick, Duncan M.
Voss, Kathleen
Pardo-Manuel de Villena, Fernando
Baric, Ralph S.
Heise, Mark T.
Gale, Michael
Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population
title Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population
title_full Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population
title_fullStr Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population
title_full_unstemmed Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population
title_short Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population
title_sort transcriptional profiles of wnv neurovirulence in a genetically diverse collaborative cross population
topic Data in Brief
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107684/
https://www.ncbi.nlm.nih.gov/pubmed/27872814
http://dx.doi.org/10.1016/j.gdata.2016.10.005
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