Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
With their essential role in inner ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by cross-linkers of the plastin, fascin, and espin families. Although mice lacking espin (ESPN) have no vestibular or...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5119939/ https://www.ncbi.nlm.nih.gov/pubmed/27811163 http://dx.doi.org/10.1083/jcb.201606036 |
_version_ | 1782469144953749504 |
---|---|
author | Krey, Jocelyn F. Krystofiak, Evan S. Dumont, Rachel A. Vijayakumar, Sarath Choi, Dongseok Rivero, Francisco Kachar, Bechara Jones, Sherri M. Barr-Gillespie, Peter G. |
author_facet | Krey, Jocelyn F. Krystofiak, Evan S. Dumont, Rachel A. Vijayakumar, Sarath Choi, Dongseok Rivero, Francisco Kachar, Bechara Jones, Sherri M. Barr-Gillespie, Peter G. |
author_sort | Krey, Jocelyn F. |
collection | PubMed |
description | With their essential role in inner ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by cross-linkers of the plastin, fascin, and espin families. Although mice lacking espin (ESPN) have no vestibular or auditory function, we found that mice that either lacked plastin 1 (PLS1) or had nonfunctional fascin 2 (FSCN2) had reduced inner ear function, with double-mutant mice most strongly affected. Targeted mass spectrometry indicated that PLS1 was the most abundant cross-linker in vestibular stereocilia and the second most abundant protein overall; ESPN only accounted for ∼15% of the total cross-linkers in bundles. Mouse utricle stereocilia lacking PLS1 were shorter and thinner than wild-type stereocilia. Surprisingly, although wild-type stereocilia had random liquid packing of their actin filaments, stereocilia lacking PLS1 had orderly hexagonal packing. Although all three cross-linkers are required for stereocilia structure and function, PLS1 biases actin toward liquid packing, which allows stereocilia to grow to a greater diameter. |
format | Online Article Text |
id | pubmed-5119939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-51199392017-05-21 Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid Krey, Jocelyn F. Krystofiak, Evan S. Dumont, Rachel A. Vijayakumar, Sarath Choi, Dongseok Rivero, Francisco Kachar, Bechara Jones, Sherri M. Barr-Gillespie, Peter G. J Cell Biol Research Articles With their essential role in inner ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by cross-linkers of the plastin, fascin, and espin families. Although mice lacking espin (ESPN) have no vestibular or auditory function, we found that mice that either lacked plastin 1 (PLS1) or had nonfunctional fascin 2 (FSCN2) had reduced inner ear function, with double-mutant mice most strongly affected. Targeted mass spectrometry indicated that PLS1 was the most abundant cross-linker in vestibular stereocilia and the second most abundant protein overall; ESPN only accounted for ∼15% of the total cross-linkers in bundles. Mouse utricle stereocilia lacking PLS1 were shorter and thinner than wild-type stereocilia. Surprisingly, although wild-type stereocilia had random liquid packing of their actin filaments, stereocilia lacking PLS1 had orderly hexagonal packing. Although all three cross-linkers are required for stereocilia structure and function, PLS1 biases actin toward liquid packing, which allows stereocilia to grow to a greater diameter. The Rockefeller University Press 2016-11-21 /pmc/articles/PMC5119939/ /pubmed/27811163 http://dx.doi.org/10.1083/jcb.201606036 Text en © 2016 Krey et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Krey, Jocelyn F. Krystofiak, Evan S. Dumont, Rachel A. Vijayakumar, Sarath Choi, Dongseok Rivero, Francisco Kachar, Bechara Jones, Sherri M. Barr-Gillespie, Peter G. Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid |
title | Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid |
title_full | Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid |
title_fullStr | Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid |
title_full_unstemmed | Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid |
title_short | Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid |
title_sort | plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5119939/ https://www.ncbi.nlm.nih.gov/pubmed/27811163 http://dx.doi.org/10.1083/jcb.201606036 |
work_keys_str_mv | AT kreyjocelynf plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid AT krystofiakevans plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid AT dumontrachela plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid AT vijayakumarsarath plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid AT choidongseok plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid AT riverofrancisco plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid AT kacharbechara plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid AT jonessherrim plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid AT barrgillespiepeterg plastin1widensstereociliabytransformingactinfilamentpackingfromhexagonaltoliquid |