Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid

With their essential role in inner ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by cross-linkers of the plastin, fascin, and espin families. Although mice lacking espin (ESPN) have no vestibular or...

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Autores principales: Krey, Jocelyn F., Krystofiak, Evan S., Dumont, Rachel A., Vijayakumar, Sarath, Choi, Dongseok, Rivero, Francisco, Kachar, Bechara, Jones, Sherri M., Barr-Gillespie, Peter G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5119939/
https://www.ncbi.nlm.nih.gov/pubmed/27811163
http://dx.doi.org/10.1083/jcb.201606036
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author Krey, Jocelyn F.
Krystofiak, Evan S.
Dumont, Rachel A.
Vijayakumar, Sarath
Choi, Dongseok
Rivero, Francisco
Kachar, Bechara
Jones, Sherri M.
Barr-Gillespie, Peter G.
author_facet Krey, Jocelyn F.
Krystofiak, Evan S.
Dumont, Rachel A.
Vijayakumar, Sarath
Choi, Dongseok
Rivero, Francisco
Kachar, Bechara
Jones, Sherri M.
Barr-Gillespie, Peter G.
author_sort Krey, Jocelyn F.
collection PubMed
description With their essential role in inner ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by cross-linkers of the plastin, fascin, and espin families. Although mice lacking espin (ESPN) have no vestibular or auditory function, we found that mice that either lacked plastin 1 (PLS1) or had nonfunctional fascin 2 (FSCN2) had reduced inner ear function, with double-mutant mice most strongly affected. Targeted mass spectrometry indicated that PLS1 was the most abundant cross-linker in vestibular stereocilia and the second most abundant protein overall; ESPN only accounted for ∼15% of the total cross-linkers in bundles. Mouse utricle stereocilia lacking PLS1 were shorter and thinner than wild-type stereocilia. Surprisingly, although wild-type stereocilia had random liquid packing of their actin filaments, stereocilia lacking PLS1 had orderly hexagonal packing. Although all three cross-linkers are required for stereocilia structure and function, PLS1 biases actin toward liquid packing, which allows stereocilia to grow to a greater diameter.
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spelling pubmed-51199392017-05-21 Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid Krey, Jocelyn F. Krystofiak, Evan S. Dumont, Rachel A. Vijayakumar, Sarath Choi, Dongseok Rivero, Francisco Kachar, Bechara Jones, Sherri M. Barr-Gillespie, Peter G. J Cell Biol Research Articles With their essential role in inner ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by cross-linkers of the plastin, fascin, and espin families. Although mice lacking espin (ESPN) have no vestibular or auditory function, we found that mice that either lacked plastin 1 (PLS1) or had nonfunctional fascin 2 (FSCN2) had reduced inner ear function, with double-mutant mice most strongly affected. Targeted mass spectrometry indicated that PLS1 was the most abundant cross-linker in vestibular stereocilia and the second most abundant protein overall; ESPN only accounted for ∼15% of the total cross-linkers in bundles. Mouse utricle stereocilia lacking PLS1 were shorter and thinner than wild-type stereocilia. Surprisingly, although wild-type stereocilia had random liquid packing of their actin filaments, stereocilia lacking PLS1 had orderly hexagonal packing. Although all three cross-linkers are required for stereocilia structure and function, PLS1 biases actin toward liquid packing, which allows stereocilia to grow to a greater diameter. The Rockefeller University Press 2016-11-21 /pmc/articles/PMC5119939/ /pubmed/27811163 http://dx.doi.org/10.1083/jcb.201606036 Text en © 2016 Krey et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Krey, Jocelyn F.
Krystofiak, Evan S.
Dumont, Rachel A.
Vijayakumar, Sarath
Choi, Dongseok
Rivero, Francisco
Kachar, Bechara
Jones, Sherri M.
Barr-Gillespie, Peter G.
Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
title Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
title_full Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
title_fullStr Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
title_full_unstemmed Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
title_short Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
title_sort plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5119939/
https://www.ncbi.nlm.nih.gov/pubmed/27811163
http://dx.doi.org/10.1083/jcb.201606036
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