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SEC23B is required for pancreatic acinar cell function in adult mice

Mice with germline absence of SEC23B die perinatally, exhibiting massive pancreatic degeneration. We generated mice with tamoxifen-inducible, pancreatic acinar cell–specific Sec23b deletion. Inactivation of Sec23b exclusively in the pancreatic acinar cells of adult mice results in decreased overall...

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Autores principales: Khoriaty, Rami, Vogel, Nancy, Hoenerhoff, Mark J., Sans, M. Dolors, Zhu, Guojing, Everett, Lesley, Nelson, Bradley, Durairaj, Haritha, McKnight, Brooke, Zhang, Bin, Ernst, Stephen A., Ginsburg, David, Williams, John A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509426/
https://www.ncbi.nlm.nih.gov/pubmed/28539403
http://dx.doi.org/10.1091/mbc.E17-01-0001
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author Khoriaty, Rami
Vogel, Nancy
Hoenerhoff, Mark J.
Sans, M. Dolors
Zhu, Guojing
Everett, Lesley
Nelson, Bradley
Durairaj, Haritha
McKnight, Brooke
Zhang, Bin
Ernst, Stephen A.
Ginsburg, David
Williams, John A.
author_facet Khoriaty, Rami
Vogel, Nancy
Hoenerhoff, Mark J.
Sans, M. Dolors
Zhu, Guojing
Everett, Lesley
Nelson, Bradley
Durairaj, Haritha
McKnight, Brooke
Zhang, Bin
Ernst, Stephen A.
Ginsburg, David
Williams, John A.
author_sort Khoriaty, Rami
collection PubMed
description Mice with germline absence of SEC23B die perinatally, exhibiting massive pancreatic degeneration. We generated mice with tamoxifen-inducible, pancreatic acinar cell–specific Sec23b deletion. Inactivation of Sec23b exclusively in the pancreatic acinar cells of adult mice results in decreased overall pancreatic weights from pancreatic cell loss (decreased pancreatic DNA, RNA, and total protein content), as well as degeneration of exocrine cells, decreased zymogen granules, and alterations in the endoplasmic reticulum (ER), ranging from vesicular ER to markedly expanded cisternae with accumulation of moderate-density content or intracisternal granules. Acinar Sec23b deletion results in induction of ER stress and increased apoptosis in the pancreas, potentially explaining the loss of pancreatic cells and decreased pancreatic weight. These findings demonstrate that SEC23B is required for normal function of pancreatic acinar cells in adult mice.
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spelling pubmed-55094262017-09-30 SEC23B is required for pancreatic acinar cell function in adult mice Khoriaty, Rami Vogel, Nancy Hoenerhoff, Mark J. Sans, M. Dolors Zhu, Guojing Everett, Lesley Nelson, Bradley Durairaj, Haritha McKnight, Brooke Zhang, Bin Ernst, Stephen A. Ginsburg, David Williams, John A. Mol Biol Cell Articles Mice with germline absence of SEC23B die perinatally, exhibiting massive pancreatic degeneration. We generated mice with tamoxifen-inducible, pancreatic acinar cell–specific Sec23b deletion. Inactivation of Sec23b exclusively in the pancreatic acinar cells of adult mice results in decreased overall pancreatic weights from pancreatic cell loss (decreased pancreatic DNA, RNA, and total protein content), as well as degeneration of exocrine cells, decreased zymogen granules, and alterations in the endoplasmic reticulum (ER), ranging from vesicular ER to markedly expanded cisternae with accumulation of moderate-density content or intracisternal granules. Acinar Sec23b deletion results in induction of ER stress and increased apoptosis in the pancreas, potentially explaining the loss of pancreatic cells and decreased pancreatic weight. These findings demonstrate that SEC23B is required for normal function of pancreatic acinar cells in adult mice. The American Society for Cell Biology 2017-07-15 /pmc/articles/PMC5509426/ /pubmed/28539403 http://dx.doi.org/10.1091/mbc.E17-01-0001 Text en © 2017 Khoriaty et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Khoriaty, Rami
Vogel, Nancy
Hoenerhoff, Mark J.
Sans, M. Dolors
Zhu, Guojing
Everett, Lesley
Nelson, Bradley
Durairaj, Haritha
McKnight, Brooke
Zhang, Bin
Ernst, Stephen A.
Ginsburg, David
Williams, John A.
SEC23B is required for pancreatic acinar cell function in adult mice
title SEC23B is required for pancreatic acinar cell function in adult mice
title_full SEC23B is required for pancreatic acinar cell function in adult mice
title_fullStr SEC23B is required for pancreatic acinar cell function in adult mice
title_full_unstemmed SEC23B is required for pancreatic acinar cell function in adult mice
title_short SEC23B is required for pancreatic acinar cell function in adult mice
title_sort sec23b is required for pancreatic acinar cell function in adult mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509426/
https://www.ncbi.nlm.nih.gov/pubmed/28539403
http://dx.doi.org/10.1091/mbc.E17-01-0001
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