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SEC23B is required for pancreatic acinar cell function in adult mice
Mice with germline absence of SEC23B die perinatally, exhibiting massive pancreatic degeneration. We generated mice with tamoxifen-inducible, pancreatic acinar cell–specific Sec23b deletion. Inactivation of Sec23b exclusively in the pancreatic acinar cells of adult mice results in decreased overall...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509426/ https://www.ncbi.nlm.nih.gov/pubmed/28539403 http://dx.doi.org/10.1091/mbc.E17-01-0001 |
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author | Khoriaty, Rami Vogel, Nancy Hoenerhoff, Mark J. Sans, M. Dolors Zhu, Guojing Everett, Lesley Nelson, Bradley Durairaj, Haritha McKnight, Brooke Zhang, Bin Ernst, Stephen A. Ginsburg, David Williams, John A. |
author_facet | Khoriaty, Rami Vogel, Nancy Hoenerhoff, Mark J. Sans, M. Dolors Zhu, Guojing Everett, Lesley Nelson, Bradley Durairaj, Haritha McKnight, Brooke Zhang, Bin Ernst, Stephen A. Ginsburg, David Williams, John A. |
author_sort | Khoriaty, Rami |
collection | PubMed |
description | Mice with germline absence of SEC23B die perinatally, exhibiting massive pancreatic degeneration. We generated mice with tamoxifen-inducible, pancreatic acinar cell–specific Sec23b deletion. Inactivation of Sec23b exclusively in the pancreatic acinar cells of adult mice results in decreased overall pancreatic weights from pancreatic cell loss (decreased pancreatic DNA, RNA, and total protein content), as well as degeneration of exocrine cells, decreased zymogen granules, and alterations in the endoplasmic reticulum (ER), ranging from vesicular ER to markedly expanded cisternae with accumulation of moderate-density content or intracisternal granules. Acinar Sec23b deletion results in induction of ER stress and increased apoptosis in the pancreas, potentially explaining the loss of pancreatic cells and decreased pancreatic weight. These findings demonstrate that SEC23B is required for normal function of pancreatic acinar cells in adult mice. |
format | Online Article Text |
id | pubmed-5509426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-55094262017-09-30 SEC23B is required for pancreatic acinar cell function in adult mice Khoriaty, Rami Vogel, Nancy Hoenerhoff, Mark J. Sans, M. Dolors Zhu, Guojing Everett, Lesley Nelson, Bradley Durairaj, Haritha McKnight, Brooke Zhang, Bin Ernst, Stephen A. Ginsburg, David Williams, John A. Mol Biol Cell Articles Mice with germline absence of SEC23B die perinatally, exhibiting massive pancreatic degeneration. We generated mice with tamoxifen-inducible, pancreatic acinar cell–specific Sec23b deletion. Inactivation of Sec23b exclusively in the pancreatic acinar cells of adult mice results in decreased overall pancreatic weights from pancreatic cell loss (decreased pancreatic DNA, RNA, and total protein content), as well as degeneration of exocrine cells, decreased zymogen granules, and alterations in the endoplasmic reticulum (ER), ranging from vesicular ER to markedly expanded cisternae with accumulation of moderate-density content or intracisternal granules. Acinar Sec23b deletion results in induction of ER stress and increased apoptosis in the pancreas, potentially explaining the loss of pancreatic cells and decreased pancreatic weight. These findings demonstrate that SEC23B is required for normal function of pancreatic acinar cells in adult mice. The American Society for Cell Biology 2017-07-15 /pmc/articles/PMC5509426/ /pubmed/28539403 http://dx.doi.org/10.1091/mbc.E17-01-0001 Text en © 2017 Khoriaty et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Khoriaty, Rami Vogel, Nancy Hoenerhoff, Mark J. Sans, M. Dolors Zhu, Guojing Everett, Lesley Nelson, Bradley Durairaj, Haritha McKnight, Brooke Zhang, Bin Ernst, Stephen A. Ginsburg, David Williams, John A. SEC23B is required for pancreatic acinar cell function in adult mice |
title | SEC23B is required for pancreatic acinar cell function in adult mice |
title_full | SEC23B is required for pancreatic acinar cell function in adult mice |
title_fullStr | SEC23B is required for pancreatic acinar cell function in adult mice |
title_full_unstemmed | SEC23B is required for pancreatic acinar cell function in adult mice |
title_short | SEC23B is required for pancreatic acinar cell function in adult mice |
title_sort | sec23b is required for pancreatic acinar cell function in adult mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509426/ https://www.ncbi.nlm.nih.gov/pubmed/28539403 http://dx.doi.org/10.1091/mbc.E17-01-0001 |
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